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Effect of Curcumin on Cancer Invasion and Matrix Metalloproteinase-9 Activity in MDA-MB-231 Human Breast Cancer Cell  

Bang, Myung-Hee (Department of Food Science and Nutrition, Dankook University)
Kim, Woo-Kyoung (Department of Food Science and Nutrition, Dankook University)
Publication Information
Journal of Nutrition and Health / v.39, no.8, 2006 , pp. 756-761 More about this Journal
Abstract
Curcumin has been known for its anti-proliferative and apoptotic effects on several cancer cells. We examined the inhibitory effects of curcumin on cancer cell adhesion, motility, invasion and matrix metalloproteinase-9 (MMP-9) activity in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured with 0, 5, 10 or $20{\mu}M$ of curcumin. Curcumin significantly inhibited the adhesion of cancer cells to the fibronectin at $20{\mu}M$ and suppressed the motility and invasion of cancer cells at all concentrations. Also, the MMP-9 activity was inhibited by curcumin, but MMP-9 protein amounts were not affected. Our data indicate that curcumin inhibits motility, invasion and MMP-9 activity of MDA-MB-231 cells. Therefore, curcumin may contribute to the potential beneficial food component to prevent the cancer metastasis in human breast cancer.
Keywords
curcumin; invasion; matrix metalloproteinase-9; metastasis; breast cancer;
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1 Yoon HS, Hong SH, Kang HJ, Xu X, Ahn SH. Survival analysis and its prognostic factors after distant relapse in breast cancer patients. J Korean Surg Soc 64: 101-108, 2003
2 Hong MK, Cho KY, Oh SJ, Kim KM, Yu SJ. Implication of the activation of matrix metalloproteinase-2 (MMP-2) on the metastasis in breast cancer. J Korean Surg Soc 62(1): 18-25, 2002
3 Chainani-Wu N. Safety and anti-inflammatory activity of curcumin: a component of tumeric (Curcuma longa). J Altern Complement Med 9(1): 161-168, 2003   DOI   ScienceOn
4 Palmieri D, Lee JW, Juliano RL, Church F. Palsminogen activator inhibitor-1 and -3 increase cell adhesion and motility of MDAMB-435 breast cancer cells. J Bio Chem 277(43): 40950-40957, 2002   DOI   ScienceOn
5 Huang Q, Shen HM, Ong CN. Inhibitory effect of emodin on tumor invasion through suppression of activator protein 1 and nuclear factor- ${\kappa}$ B. Biochem Pharmal 68: 361-371, 2004   DOI   ScienceOn
6 Garbisa S, Sartor L, Biggin S, Salvatto B, Benelli R, Albini A. Tumor gelatinases and invasion inhibited by the green tea flavanol epigalloatechin-2-gallate. Cancer 91: 822-832, 2001   DOI   ScienceOn
7 Song IH. Cancer metastasis and metastasis suppressors. Korea J Gastron 43: 1-7, 2004
8 Plater N, Prevostel C, Deroco D, Joubert D, Rochefort H, Garcia M. Breast cancer invasiveness: Correlation with protein kinase C activity and differential regulation by phobol ester in estrogen receptor -positive and -negative cells. Int J Cancer 75: 750-756, 1998   DOI   ScienceOn
9 Roy M, Chakraborty S, Siddiqi M, Bhattacharya RK. Induction of apoptosis in tumor cells by natural phenolic compounds. Asian Pac J Cancer Prev 3: 61-67, 2002
10 Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 78: 517S-520S, 2003
11 Duvoix A, Blasius R, Delhalle S, Schnekenburger M, Morceau F, Henry E, Dicato M, Diederich M. Chemopreventive and therapeutic effects of curcumin. Cancer Lett 223: 181-190, 2005   DOI   ScienceOn
12 Aggarwal S, Takada Y, Singh S, Myers JN, Aggarwal BB. Inhibition of growth and survival of human head and neck squamous cell carcinoma cells by curcumin via modulation of nuclear factor- $\kappa$B signaling. Int J Cancer 111: 679-692, 2004   DOI   ScienceOn
13 Huang MT, Lou YR, Xie JG, Ma W, Lu YP, Yen P, Zhu BT, Newmark H, Ho CT. Effect of dietary curcumin and dibenzoylmethane on formation of 7,12-dimethylbenz [a]anthracene-induced mammary tumors and lymphomas/leukemias in Sencar mice. Carcinogenesis 19: 1697-1700, 1998   DOI   ScienceOn
14 Chang C, Werb Z. The many faces of metalloproteinases: cell growth, invasion, angiogenesis and metastasis. Trends Cell Biol 11(11): S37-S42, 2001
15 Johanning GL. Modulation of breast cancer adhesion by unsaturated fatty acids. Nutrition 12: 810-816, 1996   DOI   ScienceOn
16 Rha SY, Park JO, Gong SJ, Park SH, Yoo NC, Yang WI, Roh JK, Min JS, Lee KS, Kim BS, Chung HC. Clinical relevance of urokinase-type plasminogen activator, uPA receptor, plasminogen activator inhibitor-1 co expression from tissue and serum of breast cancer as targets of biotherapy. J Korean Cancer Assoc 31(2): 56-266, 1999
17 Tourkina E, Gooz P, Oates JC, Ludwicka Bradley A, Silver RM, Hoffman S. Curcumin-induced cytotoxicity: induction of apoptosis through generation of reactive oxygen species, down-regulation of Bcl-XL and IAP, the release of cytochrome c anc inhibition of Akt. Carcinogenesis 24: 1199-1208, 2003   DOI   ScienceOn
18 Holy J. Curcumin inhibits cell motility and alters microfilament organization and function in prostate cancer cells. Cell Motil Cytoskeleton 58: 253-268, 2004   DOI   ScienceOn
19 Chen HW, Yu SL, Chen JJ, Li HN, Lin YC, Yao PL, Chou HY, Chien CT, Chen WJ, Lee YT, Yang PC. Anti-invasive gene expression profile of curcumin in lung adenocarcinoma based on a high throughput microarray analysis. Mol Pharmacol 65(1): 99-110, 2004   DOI   ScienceOn
20 Liotta LA. Cancer cell invasion and metastasis. Sci Am 266: 34-41, 1992
21 Ohashi Y, Tsuchiya Y, Koizumi K, Sakurai H, Saiki I. Prevention of intrahepatic metastasis by curcumin in an orthotopic implantation model. Oncology 65: 250-258, 2003   DOI
22 Thant AA, Nawa A, Kikkawa F. Ichigotani Y, Ahang Y, Sein TT, Amin AR, Hamaguchi M. Fibronectin activates matrix metalloproteinase-9 secretion via the MEK1-MARK and the PI3K-Akt pathways in ovarian cancer cells. Cli Exp Metastasis 18(5): 423-428, 2000   DOI   ScienceOn
23 Stracker ML, Engel JD, Wilson LW, Rechler MM, Liotta LA, Schiffmann E. The Type I insulin-like growth factor receptor is a motility receptor in human melanoma cells. J Bio Chem 264(36): 21544-21549, 1989
24 Cheng AL, Hsu CH, Lin KJ, Hsu MM, Ho YF, Shen TS, Ko JY, Lin JT, Lin BR, Ming-Shiang W, Yu HS, Jee SH, Chen GS, Chen TM, Chen CA, Lai MK, Pu YS, Pan MH, Wang YJ, Tsai CC, Hsieh CY. Phase I clinical trial of curcumin, a chemopreventive agent, in patients with high-risk or pre-malignat lesions. Anticancer Res 21: 2895-2900, 2001
25 Waladkhani AR, Clemens MR. Effect of phytochemicals on cancer development. Int J Mol Med 1: 747-753, 1998
26 Lee HY, Bae GU, Jung ID, Lee JS, Kim YH, Noh SH, Stracke ML, Park CG, Lee HW, Han JW. Autotaxin promotes motility via G protein-coupled phosphoinostide 3-kinase ${\gamma}$ in human melanoma cells. FEBS Letters 515: 137-140, 2002   DOI
27 Kim SY, Jung SH, Lim HS. Curcumin is a potent broad spectrum inhibitor of matrix metalloproteinase gene expression in human astroglioma cells. Biochem Biophys Res Commun 337(2): 510-516, 2005   DOI   ScienceOn
28 Shah BH, Nawaz Z, Petani SA, Roomi A, Mahmood H, Saeed SA, Gilani AH. Inhibitory effect of curcumin, a food spice from tumeric, on platelet-activating factor and arachidonic acid-mediated platelet aggregation through inhibition of thromboxane formation and $Ca^{2+}$ signaling. Biochem Pharmacol 58: 1167-1172, 1999   DOI   ScienceOn
29 Woo MS, Jung SH, Kim SY, Hyun JW, Ko KH, Kim WK, Kim HS. Curcumin suppresses phobal ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in Human astroglioma cells. Biochem Biophys Res Commun 335: 1017-1025, 2005   DOI   ScienceOn