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Effects of Portulaca Oleracea L. Extract on Lipolysis and Hormone Sensitive Lipase (HSL) Gene Expression in 3T3-L1 Adipocytes  

Lee, Mak-Soon (Department of Food and Nutritional Sciences, Ewha Womans University)
Kim, Chong-Tai (Biosystems Engineering Team, Korea Food Research Institute)
Kim, Chul-Jin (Biosystems Engineering Team, Korea Food Research Institute)
Cho, Young-Jin (Biosystems Engineering Team, Korea Food Research Institute)
Kim, Yang-Ha (Department of Food and Nutritional Sciences, Ewha Womans University)
Publication Information
Journal of Nutrition and Health / v.39, no.8, 2006 , pp. 742-747 More about this Journal
Abstract
This study was performed to investigate the lipolytic effects of Portulaca oleracea L. extract in 3T3-L1 adipocytes. The Portulaca oleracea L. was extracted with extrusion method using twin-screw extruder under $58{\sim}60rpm$ screw speed, $4{\sim}5kg/hr$ feed rate, $140^{\circ}C$ extrusion temperature. The lipolytic action of Portulaca oleracea L. extract was estimated by measuring the amount of glycerol and free fatty acids (FFA) released from 3T3-L1 adipocytes and by measuring the cellular lipid content in 3T3-L1 adipocytes. The hormone sensitive lipase (HSL) mRNA level was analyzed using quantitative real-time PCR. The Portulaca oleracea L. extract at 1 to $100{\mu}g/ml$ suppressed lipid accumulation. The release of glycerol and FFA into the medium, and the mRNA level of HSL were significantly increased by the addition of Portulaca oleracea L. extract at dose-dependent manner. In conclusion, the Portulaca oleracea L. extract was suggested to have the lipolytic effect through release of lipolytic products (FFA and glycerol) of triacylglyceride to the culture medium and suppression of lipid accumulation via up-regulation of HSL gene expression in 3T3-L1 adipocytes.
Keywords
Portulaca oleracea L; 3T3-L1 adipocytes; lipolytic effect; HSL gene expression;
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1 Bea KH. The medicinal plants of Korea. Kyohaksa Publishing Co., Seoul, p.129, 1999
2 Frayn KN, Coppack SW, Fielding BA, Humphreys SM. Coordinated regulation of hormone-sensitive lipase and lipoprotein lipase in human adipose tissue in vivo: implications for the control of fat storage and fat mobilization. Adv Enzyme Regul 35: 163-178, 1995   DOI   ScienceOn
3 Rached AN, Afifi FU, Disi AM. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J Ethnopharmacol 88: 131-136, 2003   DOI   ScienceOn
4 Omara-Alwala TR, Mebrahtu T, Prior DE, Ezekwe MO. Omega three fatty acids. in purslane (Portulaca oleracea) tissues. J Am Oil Chem Soc 68(3): 198-199, 1991   DOI
5 Parry O, Marks JA, Okwuasaba FK. The skeletal muscle relaxant action of Portulaca oleracea: role of potassium ions. J Ethnopharmacol 40(3): 187-194, 1993   DOI   ScienceOn
6 Moreno-Aliaga MJ, Matsumura F. Endrin inhibits adipocyte differentiation by selectively altering expression pattern of CCAAT/ enhancer binding protein-alpha in 3T3-L1 cells. Mol Pharmacol 56(1): 91-101, 1999   DOI
7 Lee TB. Illustrated Flora of Korea. Hyangmunsa Publishing Co., Seoul, pp.324, 1999
8 Frayn KN, Coppack SW, Fielding BA, Humphreys SM. Coordinated regulation of hormone-sensitive lipase and lipoprotein lipase in human adipose tissue in vivo: implications for the control of fat storage and fat mobilization. Adv Enzyme Regul 35: 163- 178, 1995   DOI   ScienceOn
9 Morimoto C, Kameda K, Tsujita T, Okuda H. Relationships between lipolysis induced by various lipolytic agents and hormonesensitive lipase in rat fat cells. J Lipid Res 42(1): 120-127, 2001
10 Holm C, Osterlund T, Laurell H, and Contreras JA. Molecular mechanisms regulating hormone-sensitive lipase and lipolysis. Annu Rev Nutr 20: 365-393, 2000   DOI   ScienceOn
11 Ahn IS, Do MS, Kim SO, Jung HS, Kim YI, Kim HJ, Park KY. Antiobesity effect of Kochujang (Korean fermented red pepper paste) extract in 3T3-L1 adipocytes. J Med Food 9(1): 15-21, 2006   DOI   ScienceOn
12 Oh KB, Chang IM, Hwang KJ, Mar W. Detection of antifungal activity in Portulaca oleracea by a single-cell bioassay system. Phytother Res 14(5): 329-332, 2000   DOI   ScienceOn
13 Peng PC, Haynes LJ, Magnus KE. High concentration of (-)- Noraderenaline in Portulaca oleracea L. Nature 191: 1108, 1961
14 Stralfors P, Bjorgell P, Belfrage P. Hormonal regulation of hormone-sensitive lipase in intact adipocytes: identification of phosphorylated sites and effects on the phosphorylation by lipolytic hormones and insulin. Proc Natl Acad Sci 81: 3317-3321, 1984
15 Yook CS. Coloured medicinal plants of Korea. Academic Publishing Co., Seoul, pp.164, 1989
16 Lim MK, Kim MR. Antimicrobial activity of methanol extract from Soibirhym (Portulaca oleracea) against food spoilage diseage microoganisms and the composition of the extract. Korean J Soc Food Cookery Sci 17: 565-570, 2001
17 Langin D, Holm C, Lafontan M. Adipocyte hormone-sensitive lipase: a major regulator of lipid metabolism. Proc Nutr Soc 55: 93-109, 1996
18 Habtemariam S, Harvey AL, Waterman PG. The muscle relaxant properties of Portulaca oleracea are associated with high concentrations of potassium ions. J Ethnopharmacol 40(3): 195- 200, 1993   DOI   ScienceOn
19 Sakai N, Inada K, Okamoto M, Shizuri Y, Fukuyama Y. Portuloside A. A monoterpene glucoside, from Portulaca oleracea. Phytochemistry (Oxford) 42(6): 1625-1628, 1996   DOI   ScienceOn
20 Liu L, Howe P, Zhou YF, Xu ZQ, Hocart C, Zhan R. Fatty acids and beta-carotene in australian purslane (Portulaca oleracea) varieties. J Chromatogr 893(1): 207-213, 2000   DOI
21 Green A, Rumberger JM, Stuart CA, Ruhoff MS. Stimulation of lipolysis by tumor necrosis factor-alpha in 3T3-L1 adipocytes is glucose dependent: implications for long-term regulation of lipolysis. Diabetes 53(1): 74-81, 2004   DOI   ScienceOn
22 Karimi G, Hosseinzadeh H, Ettehad N. Evaluation of the gastric antiulcerogenic effects of Portulaca oleracea L. extracts in mice. Phytother Res 18(6): 484-487, 2004   DOI   ScienceOn
23 Mohamed AI, Hussein AS. Chemical composition of purslane (Portulaca oleracea). Plant Foods Hum Nutr 45(1): 1-9, 1994   DOI
24 Malek F, Boskabady MH, orushaki MT, Tohidi M. Bronchodilatory effect of Portulaca oleracea in airways of asthmatic patients. J Ethnopharmacol 93: 57-62, 2004   DOI   ScienceOn