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Effect of Docosahexaenoic Acid (DHA) on the Apoptosis of Human Endothelial ECV304 Cells  

Kim Young-Youn (Division of Nutritional Medicine, Family Health and Welfare Center(FHWC), Sungshin Women's University)
Kim Hyo-Sook (Division of Nutritional Medicine, Family Health and Welfare Center(FHWC), Sungshin Women's University)
Kim Mae-Ha (Department of Food and Nutrition, Sungshin Women's University)
Jang Soo-Jeong (Department of Food and Nutrition, Sungshin Women's University)
Lee Myoung-Sook (Division of Nutritional Medicine, Family Health and Welfare Center(FHWC), Sungshin Women's University)
Publication Information
Journal of Nutrition and Health / v.39, no.4, 2006 , pp. 357-365 More about this Journal
Abstract
DHA, one of w-3 fatty acids, modulates cell growth or death though the changes of apoptotic signaling in human endothelial ECV304 cells. We investigated the effects of DHA on the changes of apoptotic signaling in human vascular endothelial ECV304 cells using lipid peroxidation (LPO) metabolites. LPO could be originated by dietary polyunsaturated fatty acids such as linoleic acid(LA), arachidonic acid(AA) and docosahexaenoic acid (DHA). DHA caused cell death of ECV304 cells compared to LA, AA or control as evidenced by changes in cell morphology and MTT assay. LPO levels was significantly elevated by 10 fold in DHA-treated ECV 304 cells and caspase-3 activity was increased by DHA corresponding to increasing incubation times compared to control. One of reasons of the cell death in DHA-treated ECV304 cells could be expected that caspase activity, marker for mitochondrial damages, might be triggered by the increasing LPO levels. Our results strongly indicated that DHA induced LPO production has an important role on apoptotic signaling pathway in ECV304 cells. LPO production in endothelial cells which was metabolized by oxidation of dietary PUFA, might be one of risk factors in the initial progression of atherosclerosis.
Keywords
DHA; LA; AA; apoptosis; human endothelial ECV304 cells; caspase-3; mitochondrial damage;
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1 Nancy D Turner, Jianhu Zhang, Laurie A Davidson, Joanne R Lupton, Robert S Chapkin. Oncogenic ras alters sensitivity of mouse colonocytes to butyrate and fatty acid mediated growth arrest and apoptosis. Cancer Letters 186: 29-35, 2002   DOI   ScienceOn
2 통계청. 1998 & 2003
3 Salem N Jr, Pawloshy R and Hibbein WJ. In vivo conversion of linoleic acid to arachidonic acid in human adults. Prostaglandin Leukotriens and Essential Fatty Acids 60(586): 407-410, 1999   DOI   ScienceOn
4 Huff MW, Telford DE, Edmonds BW, McDonald CG, Evans AJ. Lipoprotein lipase, lipoprotein density gradient profile and LDL receptor activity in miniatire pigs fed fish oil and corn oil. Biochem BiophysActa 1210: 113-122, 1993
5 Spady DK. Regulatory effects of individual n-3 polyunsaturated fatty acids on LDL transport in the rat. J Lipid Res 34: 1337- 1346, 1993
6 Napolitano M, Bravo E, Avella M, Chico Y, Ochoa B, Botham KM, Rivabene R. The fatty acid composition of chylomicron remnants influences their propensity to oxidate, Nutrition. Metabolism and Cardiovascular Diseases 14(5): 241-247, 2004   DOI   ScienceOn
7 Thomas SR, Stocker R. Molecular action of vitamin E in lipoprotein oxidation: Implications for atherosclerosis. Free Radical Biology & Medicine, 28(12): 1795-1805, 2000   DOI   ScienceOn
8 Xiaoling Qu, Leuting Qing. Abrin Induces HeLa Cell Apoptosis by Cytochrome c Release and Caspase Activation. J Bio & Molecular Biology 37(4): 445-453, 2004
9 Terano T, Shiina T, Yamamoto K, Ban T, Hirai A, Tamura Y, Saito Y, Kitagawa M. Eicosapentaenouc acid and docosahexaenoic acid inhibit DNA synthesis through inhibiting cdk 2 kinase in vascular smooth muscle cells. Ann NY Acad Sci 811: 369-377, 1997   DOI
10 Jang SD, Rho SN. Effect of Dietary perilla Seed Oil on Lipid Metabolism in Rat. Korean J Nutrition 24(5): 408-419, 1991
11 Hartley JA, Gibson NW, Qwelling LA, Yuspa SH. The association of DNA strand breaks with accelerated terminal differentiation in mouse epidermal cells exposed to tumor promoters. Cancer Res 45: 4864-4871, 1985
12 Steller H. Mechanism and genes of cellular suicide. Science 267: 1445-1449, 1995   DOI
13 Kayo Arita, Hirotsugu Kobuchi, Toshihiko Utsumi, Yoshiki Takehara, Jitsuo Akiyama, Alan A Horton, Kozo Utsumi. Mechanism of apoptosis in HL-60 cells induced by n-3 and n-6 polyunsaturated fatty acids. Biochemical Pharmacology 62: 821-828, 2001   DOI   ScienceOn
14 Cornwell DG, Hutter jj, Milo GE, Panganamala RV, Sharma HM, Geer JC. Polyunsaturated fatty acids, vitamin E and the proliferation of aortic smooth muscle cells. Lipids 14: 194-207, 1979   DOI   ScienceOn
15 Diep QN, Touyz RM, Schiffrin EL. Docosahexaenoic acid, a peroxysome proliferator-activated receptor-alpha ligand, induces apoptosis in vascular smooth muscle cells by stimulation of p-38 mitogen-activated protein kinase. 36: 851-855, 2000   DOI
16 Salem N Jr, Litman B, Kim HY, Gawrisch K. Mechanism of action of DHA in nervous system. Lipid 36(9): 945-959, 2001   DOI   ScienceOn
17 Kim WS, Kim YS. Paraoxogenase Polymorphism as a New Genetic Maker of Atherosclerosis. Journal of the Geological Society of Korea 6(2): 81-86, 1996
18 이명숙. 한국인의 지질권장량, In: 아포지단백질대사, pp.257- 307, 효일출판사, 2000
19 Jaruga E, Sokal A, Chrul S, Bartosz Z. Apoptosis-independent alteration in membrane dynamics induced by curcumin. Exp Cell Res 245: 303-312, 1998   DOI   ScienceOn
20 Omega-3 fatty acids: Epidemiological aspects of ${\omega}$-3 fatty acids, In; Handbook of Lipid in Human Nutrition, pp.75-99, CRC press, 1996
21 Moriguchi T, Loewke J, Garrision M, Catalan CJ, Salem N Jr. Reversal of DHA deficiency in the rat brain, retina, liver and serum. J of Lipid Research 42: 429-427, 2001
22 Yang SJ, Kim OY, Kwon SJ, Lee JH, Jung NS, Lee HC, Huh KB. Influence of alcohol consumption and smoking habits on cardiovascular risk factors and antioxidant status in healthy men. Korean J of Medicine 56(4): 437-449, 1999
23 Smit MJ, Verkade HJ, Havinga R, Vonk RJ, Scherphof GL, In't Veld G, Kuipers F. Dietary fish oil potentiates bile acid-induced cholesterol secretion into bile in rats. J Lipid Res 33: 77-88, 1992
24 Schneider et al. Two distinct pathways of formation od 4-HNE. JBC 276(24): 20831-20838, 2001   DOI   ScienceOn
25 Easterbauer H et al. Chemistry and biochemistry of 4-HNE, MDA and related aldehyde. Free Radic Bio Med 11: 81-128, 1991   DOI   ScienceOn
26 Choi YS, Cho SH, Im JK. Lipid peroxidation and Vitamins E and A Levels in Tissues of Rat Fed Fish Oil or Soybean Oil Supplemented with Vitamin E. Korean J Nutrition 28(10): 967- 975, 1995
27 Sylvia A Rabacchi, Wilma J Friedman, Michael L, Shelan ski, Carol M Troy. Divergence of the apoptotic pathways induced by 4-hydroxynonenal and amyloid ${\beta}$-protein. Neurobiology of Aging 25: 1057-1066, 2004   DOI   ScienceOn
28 Tripody A, Loria P, Dilengite MA, Carulli N. Effect of fish oil and coconut oil diet on the LDL receptor activity of rat liver plasma membrane. Biochem Biophysic Acla 1083: 293-334, 1991
29 Igarashi M, Miyazawa T. Do conjugated eicosapentaenoic acid acd conjugated docosahexaenoic acid induce apoptosis via lipid peroxidation in cultured human tumor cell? Biochem Biophys Res Commun 270: 649-656, 2000   DOI   ScienceOn
30 Oh HH, Mun HJ, Lee MY. Effect of CLE (Conjugated Linoleic Acid) on the Anti-atherosclerotic Factor in Human Hepatoma HepG2 Cells. Korean J Nutrition 37(3): 82-192, 2004
31 Meade EA et al. Prostagladin and related compounds: Lipid messenger with any actions, In; Lipid Second Messenger, Bell RM, Exton JH and Prescott SM, pp.285-299, Prenum Press, New York 1996
32 Diep QN, Intengan HD, Schiffrin EL. Endothelin-1 attenuates ${\omega}$3 fatty acid-induced apoptosis by inhibition of caspase-3. Hypertention 35: 287-291, 2000   DOI
33 Lee CH, Chung MC, Lee HJ, Kho YH. An apoptosis regulator isolated from petasites japonicum. Korean J Food Sci Technol 32(2): 448-453, 2000
34 보건복지부. 1996-1998
35 Woollet LA, Spady DK, Dietschy JM. Saturated and unsaturated fatty acids independently regulate low density lipoprotein receptor activity and production rate. J Lipid Res 33: 77-88, 1992
36 이명숙. 질병과 지단백질, In: 아포지단백질대사, pp.205- 245, 효일출판사, 2000