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Effects of Curcumin on Apoptosis in SW480 Human Colon Cancer Cell Line  

최옥숙 (단국대학교 자연과학부 식품영양학)
김우경 (단국대학교 자연과학부 식품영양학)
Publication Information
Journal of Nutrition and Health / v.37, no.1, 2004 , pp. 31-37 More about this Journal
Abstract
Curcumin, a natural compound extracted from rhizomes of Curcuma longa, has been shown to possess potent anti-inflammatory and anti-tumor activity. The mechanism by which curcumin initiates apoptosis remains poorly understood. In this study, we investigated the effects of curcumin on caspase-3 activity and protein expression of procaspase-3, Bcl-2, Bax, total Akt and phosphorylated Akt in SW480 human colon cancer cell. We cultured SW480 cells in the presence of various concentrations (0, 10, 20 or 30 uM) of curcumin. Curcumin inhibited colon cancer cell growth in a dose-dependent manner (p < 0.05). Caspase-3 activity was significantly increased dose-dependently in cells treated with curcumin (p < 0.05), concisely procaspase-3 expression was significantly decreased. Bcl-2 levels were decreased dose-dependently in cells treated with curcumin (p < 0.05), but Ben remained unchanged. In addition, phosphorylated Akt levels and total Akt levels were markedly lower in cells treated with 20 uM of curcumin treatment (p < 0.05), In conclusion, we have shown that curcumin inhibits cell growth and induces apoptosis in SW480 human colon cancer cell lines via Akt signal pathway.
Keywords
curcumin; apoptosis; caspase-3; Bcl-2; Bax; Akt;
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Times Cited By KSCI : 4  (Citation Analysis)
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