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Age-related Changes of DNA Damage and Antioxidative Capacity in Rats  

노경아 (이화여자대학교 식품영양학과)
김미경 (이화여자대학교 식품영양학과)
Publication Information
Journal of Nutrition and Health / v.35, no.3, 2002 , pp. 279-290 More about this Journal
Abstract
This study was performed to assess age-related changes in DNA damage and antioxidative capacity in 4, 8, 12, 16, 20, and 24 months old Sprague-Dawley male rats. The following were measured the degree of oxidative DNA damage as indicated by levels of 8-hydroxy-2'-deoxyguanosine (80HdG) in the kidney ; the peroxidized lipid concentrations in the plasma and the liver, as indicated by the levels of thiobarbituric acid reactive substances (TBARS); and the levels of antioxidant enzyme activities in the erythrocytes and the liver. Both body weight (BW) and epididymal fat pad (EFP) weight per BW increased with age until 16 months, then decreased slightly from 20 to 24 months. However, the weights of the liver, kidney and spleen per BW decreased with age. Concentrations of 8-OHdG in the kidney increased with age, only slightly front 4 to 16 months, and then markedly from 16 to 24 months. TBARS concentrations in the plasma and liver were shown to increase with age, being lowest in the 4 month-old group and highest in the 24 month-old group. Superoxide dismutase (SOD) activity in the erythrocytes increased with age Catalase activity in the erythrocytes increased from 4 to 16 months, then decreased from 20 to 24 months. Glutathione peroxidase (GSH-Px) activity in the erythrocytes showed no age-related change. Liver SOD activity decreased with age, particularly from 16 to 20 months, but catalase and GSH-Px activities in the liver showed no significant changes. These results showed that during the normal aging of SD rats, DNA damage in the kidney and TBARS concentrations in the plasma and liver increased with age, particularly after 16 months, and the imbalance of antioxidative enzyme activities in the erythrocytes accelerated with age.
Keywords
age-related changes; DNA damage; antioxidative capacity;
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1 Flohe L. Determination of glutathione peroxidase. CRC Handbook of Free Radicats and Antioxidations in Biomedicine pp.281-286,1992
2 Wiseman H, HaBiweH B. Damage to DNA by reactive oxygen and nitrogen spedes: role in inflammatory disease and progression to cancer. Biochem J 313: 17-29,1996   DOI
3 Fraga CG, Shigenaga MK, Park JW, Degan P, Ames BN. Oxid a-tive damage to DNA during aging: 8-hydroxy-2'deoxyguano-sine in rat organ DNA and urine. Proc Natt Acad Sci (USA) 87: 4533-4537,1990   DOI   ScienceOn
4 Flohe L, tting F. Superoxide dismutase assay, In: Methods in Enzymology, vol 105 'Oxygen Radicals in Biological Systems ed. Lester Packer, pp.93-114, Academic Press, 1984   DOI
5 Dahn HC, Benedetti MS, Dostert P. Differential Changes in superoxide dismutase activity in brain and liver of old rats and mice. J Neurochem 40: 1003-1007,1983   DOI   PUBMED
6 King CM, Bristow-Ciaig HE, Gillespie ES, Barnett YA. In vivo antioxidant status, DNA damage, mutation and DNA repair capadty in cultured lymphocytes from healthy 75-to 80-yearold humans. Mutat Res 377(1): 137-147,1997   DOI
7 Shigenaga MK, Gimeno CJ, Ames BN. Urinary 8-hydroxy-2'deo-xyguanosine as a biological marker of in vivo oxidative DNA damage. Proc Natl Acad Sci (USA) 86: 9677-9701,1989   DOI   ScienceOn
8 Saul RL, Ames BN. Background levels of DNA damage in the population, In: Simic MG, Grossman L, Upton AC(ed.), Mechanisms of DNA Damage and Repair, Plenum. New York, pp.113-129
9 Demple B, Harrison L. Repair of oxidative damage to DNA: Enzymology and Biology. Annu Rev Biochem 63: 915-48, 1994   DOI   ScienceOn
10 Narath E, Skalicky M, Villdik A. Vountary and forced exercise influence the survival and body composition of ageing male rats differently. Experimental Gerontotogy 36: 1699-1711, 2001   DOI   ScienceOn
11 Paek SK, Kim HY, Yang SD, Song CW, Shin TK, Han SS. The effects of Opuntia ficus-indica fmit powder on andoxidant parameters in senescence accelerated mouse (SAM). Kor J Gerontol 9(1): 70-77,1999
12 Connan B, Michel JB. Glomerular filtration, renal blood flow, and solute excretion in consdous aging rats. Am J Physiol 253(4Pt2): R555-560, 1987
13 Kim JW, No JK, Ikeno Y, Yu BP, Choi JS, Yokozawa T, Chung HY. Age-related changes in redox status of rat serum. Archives of Gerontology and Geriatrics 34: 9-17, 2002   DOI   ScienceOn
14 Husain S R, Cillard J, Cillard P. Hydroxyl radical scavenging activIty of flavonoids. Pfiytochemistry 26(9) : 2489-2491,1987   DOI   ScienceOn
15 Halliwell B. Antioxidant characterization: Methodology and mechsmism. Biochemical Pharmacohgy 49(10): 1341-1348,1995   DOI   PUBMED   ScienceOn
16 Jang IS, Chae KR, Cho JS. Effects of age and strain on small intestinal and hepatic antioxidant defense enzymes m Wistar and Fisher 344 rats. Mechanisms of Ageing and Development 122: 561-570, 2001   DOI   ScienceOn
17 Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8-hydroxydeoxyguanosine excretion in human: influence of smoking, gender and body mass index. Carcmogenesis 13(12): 2241-2247, 1992   DOI   ScienceOn
18 Buddngham KW. Effect of dietary polysatuiated/saturated fatty add ratio and dietary vitamin E on lipid peroxidation in the rat. J Nutr 115: 1425-1435,1985
19 Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci (USA) 90: 7915-7922,1993   DOI   ScienceOn
20 Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 193: 265-275, 1951
21 Lee YG, Cho SR, Lee DW. The influence of ginseng on the aging of senescence accelerated mouse. Kor J Gerontol 5(1): 1-7, 1995
22 Kim SH, Kim HY. No-Wha(Aging), Min-um-Sa, 1995
23 Choi JH, Kim DW, Kim KS, Kim CM, Baek YH. Effect of reed root extract (RGE) on leaming and memory impainnent animal model SAMP8 II. Feeding effect of RRE on Oxygen Radicals and their scavenger enzymes of SAMP8 brain. Kor J Gerontol 7(3): 23-28,1997
24 Muchova J, Sustrova M, Garaiova I, Liptakova A, Blazicek P, Kvasnicka P, Pueschel S, Durackova Z. Influence of age on activities of antioxidant enzymes and lipid peroxidation products in erythrocytes and neutrophils of Down syndrome paHents. Free Radic Biol Med 31(4): 499-508, 2001   DOI   ScienceOn
25 Schmerold I, Niedennuller H. Levels of 8-hydroxy-2'-deoxygua- nosine in cellular DNA from 12 tissues of young and old Sprague-Dawley rats. Experimental Gemntology 36(8): 1375-1386, 2001   DOI   ScienceOn
26 Flohe L, Gunzler WA. Assays of glutathione peroxidase, In: Methods in Enzymology, vol 105 'Oxygen Radicals in Biological Systems' ed. Lester Packer, pp.114-121, Academic Press, 1984
27 Kasapoglu M, Ozben T. Alteradons of andoxidant enzymes and oxidative stress markers in aging. Experanental Gerontology 36: 209-220, 2001   DOI   ScienceOn
28 Inal ME, Kanbak G, Sunal E. Antioxidant enzyme acHvities and malondialdehyde levels related to aging. Ctinica Chimica Acta 305: 75-80, 2001   DOI   ScienceOn
29 Hannan D. Free radical theory of aging. Mutation Res 275: 257-266,1992   DOI   ScienceOn
30 Choi JH, Kim DW, Kim JH, Kim HS. Effct of red ginseng ex tract (RGE) on leaming and memory impairment animal model SAMP8 II. Feeding effect of RGE on Oxygen Radicals and their scavenger enzymes of SAMP8 brain. Kor J Gerontol 7(2): 71-75,1997
31 Shibutani S, Takeshita M, Grollman AP. Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG. Nature (London) 349: 431-434,1991   DOI   ScienceOn
32 Johansson LH, Borg LA. A spectrophotometric method for determination of catalase activity in small tissue samples. AnatyticaI Biochemistry 174: 331-336,1988   DOI   ScienceOn
33 Yagi K. Assay for blood plasma or serum, In: Methods in Enzymology, Academic Press Inc. NY vol 105, pp.328-331, 1984
34 Comian B, Pratz J, Poujeol P. Changes in anatoiny, glomerular filtration, and solute excretion in aging rat kidney. Am J Physiol 248(3 Pt 2): R282-287,1985
35 Helbock HJ, Bedonan KB, Ames BN. 8-Hydroxydeoxyguanosine and 8 -hydroxyguanine as biomarkers of oxidative DNA damage, In: Methods in Enzymology vol 300 'oxidants and antioxidants part B' ed. Lester Packer, pp.156-163, Academic Piess, 1999
36 Hohe L, Becker R, Bngelius R, Lengfelder E, tting F. Convenient assays foi superoxide dismutase. CRC Handbook of free Radicats and Antwxidants in Biomedicine pp.287-293, 1992