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Antioxidant and Neuronal Cell Protective Effects of Aqueous Extracts from Lotus Leaf Tea  

Jeong, Chang-Ho (Wooyang Frozen Food Co., Ltd.)
Jeong, Hee-Rok (Dept. of Food Sci. & Techno., Gyeongsang National Univ.(Insti. of Agric. & Life Sci.))
Choi, Sung-Gil (Dept. of Food Sci. & Techno., Gyeongsang National Univ.(Insti. of Agric. & Life Sci.))
Heo, Ho Jin (Dept. of Food Sci. & Techno., Gyeongsang National Univ.(Insti. of Agric. & Life Sci.))
Publication Information
Journal of agriculture & life science / v.46, no.2, 2012 , pp. 115-127 More about this Journal
Abstract
Antioxidant and neuronal cell protective effects of aqueous extract from lotus (Nelumbo nucifera) leaf tea (LLTE) were investigated. The 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging effect, ferric reducing antioxidant power, and malondialdehyde inhibition of LLTE were increased in a dose dependent manner. Intracellular reactive oxygen species accumulation resulting from hydrogen peroxide ($H_2O_2$) treatment was significantly reduced when LLTE were present in the media compared to PC12 cells treated with $H_2O_2$ only. In neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), LLTE showed protective effect against $H_2O_2$-induced neurotoxicity. In addition, lactate dehydrogenase release into medium was also inhibited by LLTE (7.13-43.89%). Total phenolics of LLTE were 33.16 mg/g and a quercetin was identified as major phenolics (105.93 mg/100g). Therefore, above these data suggest that LLTE including quercetin may be useful in the natural antioxidant substance, and may reduce the risk of neurodegenerative disease.
Keywords
Nelumbo nucifera; Quercetin; Antioxidant; Neuronal cell protection;
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