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Antioxidant and Neuronal Cell Protective Effect of Purple Sweet Potato Extract  

Kwak, Ji-Hyun (Dept. of Food Sci. & Biotech.(Insti. of Agric. and Life Sci.), Gyeongsang National Unive.)
Choi, Gwi-Nam (Dept. of Food Sci. & Biotech.(Insti. of Agric. and Life Sci.), Gyeongsang National Unive.)
Park, Ju-Hee (Dept. of Food Sci. & Biotech.(Insti. of Agric. and Life Sci.), Gyeongsang National Unive.)
Kim, Ji-Hye (Dept. of Food Sci. & Biotech.(Insti. of Agric. and Life Sci.), Gyeongsang National Unive.)
Jeong, Hee-Rok (Dept. of Food Sci. & Biotech.(Insti. of Agric. and Life Sci.), Gyeongsang National Unive.)
Jeong, Chang-Ho (Dept. of Food Sci. & Biotech.(Insti. of Agric. and Life Sci.), Gyeongsang National Unive.)
Heo, Ho-Jin (Dept. of Food Sci. & Biotech.(Insti. of Agric. and Life Sci.), Gyeongsang National Unive.)
Publication Information
Journal of agriculture & life science / v.44, no.2, 2010 , pp. 57-66 More about this Journal
Abstract
The antioxidant and neuronal cell protective effects of water extract from purple sweet potato were investigated. The total phenolics and monomeric anthocyanin contents of purple sweet potato extract were 44.25 mg/g and 2,394 mg/L, respectively. The antioxidant activities of purple sweet potato extract were evaluated using various antioxidant tests, including 1,1-diphenyl- 2-picrylhydrazyl (DPPH), 2,2'-azino- bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, ferric reducing/antioxidant power (FRAP) and reducing power. In these assays, the extract of purple sweet potato presented significant radical scavenging activities, FRAP, and reducing power in a dose-dependent manner. MTT {3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl- tetrazoliumbromide} reduction assay showed significantly increase in cell viability when PC12 cells were pretreated with purple sweet potato extract. Because oxidative stress is also known to increase neuronal cell membrane breakdown, we further investigated by lactate dehydrogenase (LDH) and neutral red uptake assay. Purple sweet potato extract inhibited oxidative stress-induced membrane damage in neuronal cells. Therefore, these data results demonstrated that water extract of purple sweet potato have antioxidant activity and neuronal cell protective effect thus it has great potential as a natural source for human health.
Keywords
purple sweet potato; antioxidant; oxidative stress; Alzheimer's disease;
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