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http://dx.doi.org/10.7841/ksbbj.2011.26.2.165

Screening of Functional Materials from Solvent Fractions of Apple Flower Leaf Extract  

Choi, Sun-Ju (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
Cho, Eun-Ah (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
Cho, Eun-Hye (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
Jeong, Yoon-Joo (R&D Center, Cotde Co., Ltd.)
Ku, Chang-Sub (R&D Center, Cotde Co., Ltd.)
Ha, Byung-Jhip (R&D Center, Cotde Co., Ltd.)
Chae, Hee-Jeong (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
Publication Information
KSBB Journal / v.26, no.2, 2011 , pp. 165-171 More about this Journal
Abstract
Fractional solvent extraction by organic solvents such as hexane, chloroform, ethylacetate, and butanol was carried out using 70% ethanol extract of apple flower leaves. Biological activities including antioxidant, whitening, antimicrobial and anti-wrinkle activities were investigated and bio-active materials of the extracts were identified using GC/MSD. Among the tested solvent fractions, ethylacetate fraction showed the highest total polyphenol content (1218.94 ${\mu}g/mL$), and flavonoid (140 ${\mu}g/mL$). The DPPH radical scavenging activities was over 80% at a dry matterbased concentration of 200 ${\mu}g/{\mu}L$ and SOD-like activity was over 90% at 50 ${\mu}g/mL$ concentration in ethylacetate fraction that was slightly lower than of ascorbic aicd. Tyrosinase inhibition activity related to skin-whitening was over 60% by ethylacetate fraction of 100 ${\mu}g/mL$. As an anti-aging effect, elastase inhibitory activity was about 45% in ethylacetate fraction. Also, it showed a significantly antimicrobial activity against P. acenes. From GC/MSD analysis, a characteristic peak of high content in ethylacetate fraction was identified as kaempferol, which has been reported as a bioactive compound.
Keywords
apple flower leaf; kaempferol; polyphenol; solvent fraction; antioxidant;
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