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http://dx.doi.org/10.4490/algae.2018.33.11.10

A continuous-flow and on-site mesocosm for ocean acidification experiments on benthic organisms  

Kim, Ju-Hyoung (Faculty of Marine Applied Biosciences, Kunsan National University)
Kang, Eun Ju (Research Institute for Basic Science, Chonnam National University)
Kim, Keunyong (Research Institute for Basic Science, Chonnam National University)
Kim, Kwang Young (Department of Oceanography, College of Natural Sciences, Chonnam National University)
Publication Information
ALGAE / v.33, no.4, 2018 , pp. 359-366 More about this Journal
Abstract
Mesocosm experiments conducted for ecological purposes have become increasingly popular because they can provide a holistic understanding of the biological complexities associated with natural systems. This paper describes a new outdoor mesocosm designed for $CO_2$ perturbation experiments of benthos. Manipulated the carbonate chemistry in a continuous flow-through system can be parallelized with diurnal changes, while irradiance, temperature, and nutrients can vary according to the local environment. A target hydrogen ion activity (pH) of seawater was sufficiently stabilized and maintained within 4 h after dilution, which was initiated by the ratio of $CO_2$-saturated seawater to ambient seawater. Specifically, pH and $CO_2$ partial pressure ($pCO_2$) levels gradually varied from 8.05-7.28 and $375-2,691{\mu}atm$, respectively, over a range of dilution ratios. This mesocosm can successfully manipulate the pH and $pCO_2$ of seawater, and it demonstrates suitability for ocean acidification experiments on benthic communities.
Keywords
carbonate chemistry; $CO_2$ partial pressure; continuous flow-through system; ocean acidification;
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