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http://dx.doi.org/10.9724/kfcs.2011.27.4.067

Neuroprotective Effects of Extracts from Diospyros kaki L. Peel  

Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University)
Moon, Seong-Hee (Department of Food Science and Biotechnology, Kyungnam University)
Choi, Ae-Ran (Department of Food Science and Biotechnology, Kyungnam University)
Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University)
Ahn, Kwnag-Hwan (Sweet Persimmon Research Institute, Gyungsangnamdo Agricultural Research & Extension Services)
Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
Publication Information
Korean journal of food and cookery science / v.27, no.4, 2011 , pp. 67-73 More about this Journal
Abstract
This study was performed to assess the neuroprotective effects of methanolic extracts from sweet persimmon peel (PPE) against glutamate-induced cytotoxicity in hybridoma N18-RE-105 cells. The neuroprotective effects of PPE in N18-RE-105 cells were measured using the MTT reduction assay, LDH release assay, and phase-contrast microscopy. The results of the MTT reduction assay showed that treating cells with 500 ${\mu}g/ml$ PPE resulted in cell viability of 66.9%. Additionally, the morphological changes and the results of the LDH release assay showed that glutamate-induced damage to nerve cells was strongly inhibited by PPE. GSH content of N18-RE-105 cells was 3.5 ${\mu}M$ compared to that of the control, whereas pretreatment with 500 ${\mu}g/ml$ PPE increased GSH content by 4.7 ${\mu}M$. PPE was fractionated with hexane, and that layer had the highest neuroprotective effects in glutamate-stressed N18-RE-105 cells. In conclusion, our data showed that glutamate potentiated the effects of N18-RE-105 cell death by a mechanism involving oxidative stress. Therefore, PPE may be a potential candidate for prevention and therapy of neurodegenerative diseases.
Keywords
neuroprotective effect; sweet persimmon; glutamate; N18-RE-105 cells; glutathion;
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