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http://dx.doi.org/10.9799/ksfan.2022.35.1.007

Macrophage Stimulating Activity of Crude Polysaccharide on Maca (Lepidium meyenii) Varieties  

Shin, Hyun Young (Transdisciplinary Major in Learning Health Systems, Dept. of Integrated Biomedical and Life Science, Graduate School, Korea University)
Kim, Hoon (College of Biotechnology and Natural Resources, Chung-Ang University)
Jeong, Eun-Jin (Major in Food and Nutrition, Korea National University of Transportation)
Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
Publication Information
The Korean Journal of Food And Nutrition / v.35, no.1, 2022 , pp. 7-15 More about this Journal
Abstract
Maca roots (Lepidium meyenii) are an important medicinal herb that have long been used by the Andes-indigenous peoples and South Americans. In Korea, recently, it has attracted attention as a health food material because of nutritional values and physiological activities. The purpose of this study was to investigate the industrial applicability of maca (red and golden varieties; R&G) as immunostimulating materials. In the macrophage stimulating assay using RAW 264.7 cells at 125~500 ㎍/mL of non-cytotoxicity doses, G-HW showed the most potent production of TNF-α, IL-6 and nitric oxide compared to red maca or the other extracts. The general component analysis results showed that all extracts comprised more than 90% neutral sugars with small amounts of uronic acid and protein. Meanwhile, component sugar analysis showed the difference in the content of uronic acids of cold- and hot-water extract. Additionally, the further fractionation of G-HW into crude polysaccharide (G-CP) greatly enhanced the macrophage stimulating activity, and G-CP contained macromolecules over 144 kDa, comprised mainly of glucose and galacturonic acid (51.0 and 34.9%). In conclusion, crude polysaccharide from maca showed industrial applicability as immunostimulating material, and especially golden maca showed higher macrophage stimulating activity than red maca.
Keywords
maca (Lepidium meyenii); hot-water extract; crude polysaccharide; macrophage stimulating activity;
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