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http://dx.doi.org/10.9799/ksfan.2019.32.6.611

Antioxidant Activity and Tyrosinase Inhibitory Activities of Codonopsis lanceolata Extract and Solvent Fraction  

Kim, Ji Yeong (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
Lee, Min-Ki (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
Hwang, Byung Soon (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
Kim, Gi-Chang (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
Hwang, In Guk (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
Publication Information
The Korean Journal of Food And Nutrition / v.32, no.6, 2019 , pp. 611-619 More about this Journal
Abstract
The purpose of this study was to investigate the antioxidant activity and tyrosinase inhibitory activity of Codonopsis lanceolata 50% ethanol extract, and its solvent fractions (n-hexane, ethyl acetate (EA), n-butanol, water). The main components of the EA fraction were qualitatively analyzed using UPLC Q-ToF/MS. Additionally, a quantitative analysis was performed using UPLC. As a result, the total polyphenol content was 113.36 mg gallic acid/g in the EA fraction, which contained the largest amount of the C. lancolata solvent fractions. Also EA showed the highest antioxidant activity than other fractions. The IC50 of DPPH(2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity was 0.03 mg/mL and the IC50 of ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)] radical scavenging activity was 0.049 mg/mL. The EA fraction showed tyrosinase inhibitory activity than other fractions and especially inhibited monophenolase oxidase reaction higher than diphenolase oxidase reaction. The monophenolase oxidase inhibited 55% when the concentration of the EA fraction was 0.25 mg/mL. As a result of Q-ToF/MS analysis, it was confirmed that tangshenoside I and lobetyolin were the main components of EA fraction. Thus, these results suggest that C. lanceolata may be used as a potent source of cosmetic agents.
Keywords
Codonopsis lanceolata; antioxidant activity; mushroom tyrosinase; tangshenoside I; lobetyolin;
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