Browse > Article
http://dx.doi.org/10.9799/ksfan.2018.31.6.865

Neuroprotective Effects of Schisandra chinensis and Ribes fasciculatum Extract on Hydrogen Peroxide-Mediated Oxidative Stress in Neuroblastic SH-SY5Y Cell Line  

Park, Eun-kuk (Dept. of Medical Genetics, Ajou University School of Medicine)
Han, Kyung-Hoon (Research Institute, Seoul Medical Center)
Lee, Seung-Hee (Research Institute, Seoul Medical Center)
Kim, Nam-Ki (Rpbio Research Institute, Rpbio Co. Ltd)
Bae, Mun-Hyoung (Rpbio Research Institute, Rpbio Co. Ltd)
Seo, Young-Ha (Rpbio Research Institute, Rpbio Co. Ltd)
Yong, Yoon-joong (NineB Research Institute, NineB Co. Ltd)
Jeong, Seon-Yong (Dept. of Medical Genetics, Ajou University School of Medicine)
Choi, Chun-Whan (Biocenter, Gyeonggido Business and Science Accelerator)
Publication Information
The Korean Journal of Food And Nutrition / v.31, no.6, 2018 , pp. 865-872 More about this Journal
Abstract
In neuronal cell deaths, oxidative stress is normally implicated with a most of these deaths occurring in neurodegenerative disorders such as the Alzheimer's and Parkinson's diseases. In this study, the neuroprotective effects of Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts on hydrogen peroxide ($H_2O_2$)-induced oxidative stress in neuroblastic cell line were investigated. For an hour, hydrogen peroxide of $100{\mu}M$ concentration, was induced on neuroblastic cells, causing apoptic cell death. For the neuroprotection, a sample of neuroblastic cells had been pre-treated with SC and RF extracts for 24 hours before application of the hydrogen peroxide. No neurotoxic effects were observed in the cells that had been treated by SC and RF. This prove that the treatment of SC and RF extract prevented apoptotic cell death of neuroblastic cell line exposed to oxidative injury. In addition, applying both SC and RF extracts at a 7:3 ratio increased the neuronal cell survival rate, compared to individual treatments of SC and RF extract. This study suggests that SC and RF extracts may be potential therapeutic agents for the prevention of neuronal cell death.
Keywords
Schisandra chinensis; Ribes fasciculatum; neuronal cell death; neuroprotection; oxidative stress;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Baral S, Pariyar R, Yoon CS, Yun JM, Jang SO, Kim SY, Oh H, Kim YC, Seo J. 2015. Effects of Schisandra fructus 70% ethanol extract on proliferation and differentiation of human embryonic neural stem cells. Korean J Pharmacogn 46:52-58
2 Bidchol AM, Wilfred A, Abhijna P, Harish R. 2011. Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italica. FoodBioprocess Technol 4: 1137-1143   DOI
3 Byun MW, Byun EH. 2015. Immunological synergistic effects of combined treatment with herbal preparation (HemoHIM) and red ginseng extracts. J Korean Soc Food Sci Nutr 44:182-190   DOI
4 Cha JY, Yoo HR, Kim YS, Seol IC, Jo HK. 2017. Inhibition of gene associated with dyslipidemia and antioxidative effect of Artemisia iwayomogi, Curcumae Radix and Raphani Semen (ACR) on HepG2 cell model. J Korean Med 38:43-58   DOI
5 Choi JR, Choi JM, Lee S, Cho KM, Cho EJ, Kim HY. 2014. The protective effects of protocatechuic acid from Momordica charantia against oxidative stress in neuronal cells. Korean J Pharmacogn 45:11-16
6 Comporti M. 2012. Free radicals, oxidative stress, and antioxidants. J Siena Acad Sci 2:13-26   DOI
7 Giridharan VV, Thandavarayan RA, Sato S, Ko KM, Konishi T. 2011. Prevention of scopolamine-induced memory deficits by schisandrin B, an antioxidant lignan from Schisandra chinensis in mice. Free Radic Res 45:950-958   DOI
8 Jung JW, Kim SJ, Ahn EM, Oh SR, Lee HJ, Jeong JA, Lee JY. 2014. Ribes fasciculatum var. Chinense attenuated allergic inflammation in vivo and in vitro. Biomol Ther 22:547-552   DOI
9 Kim Hk, Na GM, Ye SH, Han HS. 2004. Extraction characteristics and antioxidative activity of Schiznadra chinensis extracts. J Korean Soc Food Cult 19:484-490
10 Oh DH, Koh SH, Chung B, Park KH, Kim HY, Song CW, Kim YC, Kim JH, Kim MH, Kim SH. 2006. Cytoprotective effect of 15-deoxy-delta (12,14) prostaglandin J2 (15d-PGJ2) against $H_2O_2$ induced death of neuronally-differentiated PC12 cells. J Korean Neurol Assoc 24:58-65
11 Han KH, Kim DH, Song KY, Lee SW, Han SH. 2015. The effects of methanol extract from cheonggukjang in T98G cells and early stage of focal ischemia rodent models. Korean J Food Nutr 28:965-972   DOI
12 Han KH, Lee SH, Park KS, Song KY, Kim JH, Park EK, Han SH. 2017. Protective effect of PineXol(R) against amyloid-${\beta}$-induced cell death. Korean J Food Nutr 30:1279-1285
13 Heo HJ, Kim DO, Choi SJ, Shin DH, Lee CY. 2004. Potent inhibitory effect of flavonoids in Scutellaria baicalensis on amyloid ${\beta}$ protein-induced neurotoxicity. J Agric Food Chem 52:4128-4132   DOI
14 Jeong CH, Kwak JH, Kim JH, Choi GN, Jeong HR, Heo HJ. 2010. Neuronal cell protective effects of methanol extract from Cheonggukjang using in vitro system. Korean J Food Sci Technol 42:768-772
15 Kim SY, Kim MJ, Ahn KJ, An IS, An SK. 2015. Antioxidant effects of kinetin on HaCaT human keratinocytes damaged by hydrogen peroxide ($H_2O_2$). Korean J Aesthet Cosmetol 13:59-64
16 Kim HS, Moon HK, Lee YJ, Lee CY, Hwang KH, Kim OH, Yoo IS, Jung K. 2015. Comparison of the content of shizandrin, gomisin A and gomisin N in schisandra fruit by water extraction condition. J Food Hyg Saf 30:59-64   DOI
17 Kim JH, Jeong CH, Choi GN, Kwak JH, Choi SG, Heo HJ. 2009. Antioxidant and neuronal cell protective effects of methanol extract from Schizandra chinensis using an in vitro system. Korean J Food Sci Technol 41:712-716
18 Kim K, Hahn B, Choe W. 2012. $H_2O_2$ induced two type of cell death in human hepatoma cell, Huh7. Cancer Prev Res 17:15-18
19 Lee KS, Lee EK, Seo YH, Choe SY. 2016 Effects of the mixture of fenugreek seeds and Lespedeza cuneata extracts on testosterone synthesis in TM3 cells oxidative stressed with $H_2O_2$. J Appl Biol Chem 59:305-311
20 Lee MS, Chao J, Yen JC, Lin LW, Tsai FS, Hsieh MT, Peng WH, Cheng HY. 2012. Schizandrin protects primary rat cortical cell cultures from glutamate-induced apoptosis by inhibiting activation of the MAPK family and the mitochondria dependent pathway. Molecules 18:354-372   DOI
21 Lenaz G, 1998. Role of mitochondria in oxidative stress and aging. Biochim Biophys Acta 1366:53-67   DOI
22 Wang CP, Li GC, Shi YW, Zhang XC, Li JL, Wang ZW, Ding F, Liang XM. 2014. Neuroprotective effect of schizandrin A on oxygen and glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons. J Physiol Biochem 70:735-747   DOI
23 Papa S, Skulachev VP. 1997. Reactive oxygen species, mitochondria, apoptosis and aging. pp.305-319. Springer
24 Park SH, Park SK, Ha JS, Lee DS, Kang JY, Kim JM, Lee UK, Heo HJ. 2016. Effect of gomchwi (Ligularia fischeri) extract against high glucose- and $H_2O_2$-induced oxidative stress in PC12 cells. Korean J Food Sci Technol 48:508-514   DOI
25 Piao XL, Kim HY, Yokozawa T, Lee YA, Piao XS, Cho EJ. 2005. Protective effects of broccoli (Brassica oleracea) and its active components against radical-induced oxidative damage. J Nutr Sci Vitaminol 51:142-147   DOI
26 Woo C, You JY, Jang CY, Kim HR, Shin YJ, Moon AJ, Shin SH. 2014. Protective effects of sosokmyoung-tang against Parkinson’s model in human neuroblastoma SH-SY5Y cells. J Int Korean Med 35:298-308
27 Xu X, Zhou X, Zhou XW, Zhang Z, Liao MJ, Gao Q, Luo HM. 2012. Schizandrin prevents dexamethasone-induced cognitive deficits. Neurosci Bull 28:532-540   DOI
28 Zeng KW, Zhang T, Fu H, Liu GX, Wang XM. 2012. Schisandrin B exerts anti-neuroinflammatory activity by inhibiting the toll-like receptor 4-dependent MyD88/IKK/NF-${\kappa}B$ signaling pathway in lipopolysaccharide-induced microglia. Eur J Pharmacol 692:29-37   DOI