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http://dx.doi.org/10.9799/ksfan.2018.31.6.836

Influence of High Hydrostatic Pressure Treatment after Germination on Anti-proliferation Effects of Soyasaponin-rich Fraction in Black Soybean (Glycine max L.)  

Kim, Min Young (Dept. of Food Science and Biotechnology, Chungbuk National University)
Lee, Yoon Jeong (Dept. of Food Science and Biotechnology, Chungbuk National University)
Song, Myeong Seob (Dept. of Food Science and Biotechnology, Chungbuk National University)
Oh, Hyunah (Dept. of Food Science and Biotechnology, Chungbuk National University)
Kim, Kyung Mi (National Academy of Agricultural Science, Rural Development Administration)
Kang, Tae Su (Dept. of Food Science and Biotechnology, Chungbuk Provincial University of Science and Technology)
Lee, Youn Ri (Dept. of Food and Nutrition, Daejeon Health Sciences College)
Lee, Junsoo (Dept. of Food Science and Biotechnology, Chungbuk National University)
Jeong, Heon Sang (Dept. of Food Science and Biotechnology, Chungbuk National University)
Publication Information
The Korean Journal of Food And Nutrition / v.31, no.6, 2018 , pp. 836-843 More about this Journal
Abstract
The objective of this study was to determine the effect of high hydrostatic pressure (HHP) treatment on proliferation of human cancer cell lines (MCF-7, HCT-116, PC-3 and AGS) of crude soyasaponin extracts in germinated black soybean. Black soybean was germinated and subjected to HHP, followed by preparation of crude soyasaponin extracts. Cell treatments done with extracts less than $400{\mu}g/mL$ concentrations had no significant effect on 3T3-L1 adipocyte cell viability. The inhibitory effect of crude soyasaponin extracts with germination periods and applied pressure on breast cancer cell (MCF-7), human colon cancer cell (HCT-116), human gastriccancer cell (AGS) and prostate cancer cell (PC-3) growth were investigated using MTT assay. The highest anti-proliferation of human cancer cell line of crude soyasaponin extracts was observed at 150 MPa treatment after germination for 4 days (150 MPa-Day 4). The cell viability on MCF-7, HCT-116, PC-3 and AGS cell lines of crude soyasaponin extract in 150 MPa-Day4 was 48.82%, 57.37%, 39.89% and 23.94% at $400{\mu}g/mL$, respectively. These results suggest that soyasaponin extracts from black soybean subjected to HHP after germination may mediate physiological activity.
Keywords
black soybean; soyasaponin; germination; high hydrostatic pressure treatment; anticancer activity;
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