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http://dx.doi.org/10.9799/ksfan.2018.31.4.457

Study on the Protective Mechanism of Vitamin C in the SH-SY5Y Cell Death Induced by the Streptozotocin  

Lee, Seung-Hee (Research Institute, Seoul Medical Center)
Han, Kyung-Hoon (Research Institute, Seoul Medical Center)
Kim, Hyun-Jun (Research Institute, Seoul Medical Center)
Park, Kwang-Sung (Research Institute, Seoul Medical Center)
Han, Sung-Hee (Biomedical Research Center, Korea University Anam Hospital)
Kim, Jung-Hee (Dept. of Neurosurgery, Seoul Medical Center)
Heo, Jae-Hyeok (Dept. of Neurology Seoul Medical Center)
Publication Information
The Korean Journal of Food And Nutrition / v.31, no.4, 2018 , pp. 457-463 More about this Journal
Abstract
In this study, we analyzed the protective effects of the vitamin C in the streptozotocin (STZ)-induced apoptosis using the SH-SY5Y, a neuroblastoma cell line. The cells were pretreated with the vitamin C ($100{\mu}g$) for 30 min, followed by the 24-hr treatment with the 2.5-mM STZ. The cell-viability assay using the Cell Counting Kit (CCK)-8 revealed the cell-survival rate increased by 15% following the vitamin-C pretreatment compared to the STZ-only treatment. Moreover, we conducted the western-blot analysis to determine the protective effect of the vitamin C regarding the apoptosis. Compared to those in the STZ-only-treatment group, the p-ERK and Bcl-2 expressions increased in the vitamin-C-pretreatment group, whereas the p-JNK and Bax expressions decreased. The vitamin-C pretreatment increased the expression of the SOD-1, an antioxidant enzyme, by more than 30%, indicating its protective role in the STZ-induced oxidative stress. Also, we found both the intrinsic- and extrinsic-pathway mechanisms of the STZ-induced apoptosis. The results of this study $s{\mu}ggest$ vitamin C may help prevent the neurodegenerative diseases.
Keywords
Alzheimer's disease; streptozotocin; reactiveoxygen species; vitamin C;
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1 Degenhardt K, Mathew R, Beaudoin B, Bray K, Anderson D, Chen G, Mukherjee C, Shi Y, Géliinas C, Fan Y, Nelson DA, Jin S, White E. 2006. Autophagypromotes tumor cell survival and restricts necrosis, inflammation and tumorigenesis. Cancer Cell 10:51-64   DOI
2 Du J, Cullen JJ, Buettner GR. 2012. Ascorbic acid: Chemistry, biology and the treatment of cancer. Biochim Biophys Acta 1826:443-457
3 Gross A, McDonnell JM, Korsmeyer SJ. 1999. Bcl-2 familymembers and the mitochondria in apoptosis. Genes Dev 13:1899-1911   DOI
4 Heo HJ, Lee CY. 2005. Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells. J Agric Food Chem 53:1984-1989   DOI
5 Hoyer S. 1993. Abnormalities in brain glucose utilization and its impact on cellular and molecular mechanisms in sporadic dementia of Alzheimer type. Ann N Y Acad Sci 695:77-80   DOI
6 Hutton M, Perez-Tur J, Hardy J. 1998. Genetics of Alzheimer's disease. Essays Biochem 33:117-131   DOI
7 Jeong YS, Hwang BS, Cho SM, Hwang KA, Hwang IG. 2017. Antioxidant and anti-diabetic, anti-Alzheimer activities of stem from Opuntia ficus-indica var. saboten cultivated in Jeju at harvest time. Korean J Food Nutr 30:1332-1340
8 Junod A, Lambert AE, Orci L, Pictet R, Gonet AE, Renold AE. 1967. Studies of the diabetogenic action of streptozotocin. Proc Soc Exp Biol Med 126:201-205   DOI
9 Kim MO, Jang SM. 2004. Effects of various proteins on the autoxidation of L-ascorbic acid. Korean J Food Nutr 17:294-301
10 Knott AB, Perkins G, Schwarzendacher R, Bossy-Wetzell E. 2008. Mitochondrial fragmentation in neurodegeneration. Nat Rev Neurosci 9:505-518   DOI
11 Sayers TJ. 2011. Targeting the extrinsic apoptosis signaling pathway for cancer therapy. Cancer Immunol Immunother 60:1173-1180   DOI
12 Cunnane S, Nugent S, Roy M, Courchesne-Loyer A, Croteau E, Tremblay S, Castellano A, Pifferi F, Bocti C, Paquet N, Begdouri H, Bentourkia M, Turcotte E, Allard M, Barberger-Gateau P, Fulop T, Rapoport SI. 2011. Brain fuel metabolism, aging, and Alzheimer's disease. Nutrition 27:3-20   DOI
13 Masters CL, Beyreuther K. 2006. Alzheimer's centennial legacy: Prospects for rational therapeutic intervention targeting the $A{\beta}$ amyloid pathway. Brain 129:2823-2839   DOI
14 Mattson MP, Partin J, Begley JG. 1998. Amyloid ${\beta}$-peptide induces apoptosis-related events in synapses and dendrites. Brain Res 807:167-176   DOI
15 Park KJ, Gaynor RB, Kwak YT. 2003. Heat shock protein 27 association with the $I{\kappa}B$ kinase complex regulates tumor necrosis factor ${\alpha}$-induced NF-${\kappa}$B activation. J Biol Chem 15:35272-352278
16 Reynolds A, Lauriea C, Mosleya RL, Gendelmana HE. 2007. Oxidative stress and the pathogenesis of neurodegenerative disorders. Int Rev Neurobiol 82:297-325
17 Wang C, Youle RJ. 2009. The role of mitochondria in apoptosis. Annu Rev Genet 43:95-118   DOI
18 Sisodia SS. 1999. Alzheimer's disease: Perspectives for the new millennium. J Clin Invest 104:1169-1170   DOI
19 Sotiriou S, Gispert S, Cheng J, Wang Y, Chen A, Hoogstraten-Miller S, Miller GF, Kwon O, Levine M, Guttentag SH, Nussbaum RL. 2002. Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival. Nat Med 8:514-517   DOI
20 Stanciu M, Wang Y, Kentor R, Burke N, Watkins S, Kress G, Reynolds I, Klann E, Angiolieri MR, Johnson JW, DeFranco DB. 2000. Persistent activationof ERK contributes to glutamate-induced oxidative toxicity in a neuronal cell line and primary cortical neuron cultures. J Biol Chem 275:12200-12206   DOI
21 Kwon KH, Lim HY, Chung MJ. 2014. Neuroprotective effects of bread containing Cirsium setidens or Aster scaber. J Korean Soc Food Sci Nutr 43:829-835   DOI
22 Wohaieb SA, Godin DV. 1987. Starvation-related alterations in free radical tissue defense mechanisms in rats. Diabetes 36:169-173   DOI
23 Younkin SG. 1998. The role of $A{\alpha}$42 in Alzheimer's disease. J Physiol Paris 92:289-292   DOI
24 Zheng S, Zhao M, Ren Y, Wu Y, Yang J. 2015. Sesamin suppresses STZ induced INS-1 cell apoptosis through inhibition of NF-${\kappa}$B activation and regulation of Bcl-2 family protein expression. Eur J Pharmacol 750:52-58   DOI