A Study of Antioxidant Effects of Pyracantha angustifolia(Franch.) C. K. Schneid Extract

In this study, Pyracantha angustifolia (Franch.) C. K. Schneid was extracted with 70% methanol at room temperature for 48 hrs and concentrated under reduced pressure to measure its total polyphenol contents; furthermore, the effect of electron donating ability was examined. Methylene chloride, ethyl acetate, and methanol were used to fractionate the extract to testify total polyphenol contents, electron donating abilities, the removal abilities of superoxide radical as well as hydrogen peroxide. The total polyphenol contents were $2007.30{\pm}109.28{\mu}g\;GAE/mL$ in 70% methanol extract, $273.39{\pm}10.19{\mu}g\;GAE/mL$ in methylene chloride fraction, $80.57{\pm}0.64{\mu}g\;GAE/mL$ in ethyl acetate fraction, and $1,160.87{\pm}44.71{\mu}g\;GAE/mL$ in methanol fraction. The total polyphenol contents showed significant differences (p<0.05) between the solvents. The electron donating ability was $79.07{\pm}7.31%$ for 70% methanol extract, $22.34{\pm}0.64%$ for methylene chloride fraction, $5.33{\pm}0.28%$ for ethyl acetate fraction, and $32.26{\pm}1.10%$ for methanol fraction. The electron donating abilities were significantly different(p<0.05) between the solvents. The removal ability of superoxide radical was $0.018{\pm}0.003$ for 70% methanol extract, $0.007{\pm}0.002$ for methylene chloride fraction, $0.0147{\pm}0.003$ for ethyl acetate fraction, and nothing for methanol fraction. The measurement of hydrogen peroxide decomposition was $0.022{\pm}0.0046$ for 70% methanol extract, $0.0027{\pm}0.0015$ for methylene chloride fraction, $0.0037{\pm}0.0012$ for ethyl acetate fraction, and $0.0009{\pm}0.0001$ for methanol fraction.

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