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http://dx.doi.org/10.9799/ksfan.2017.30.6.1279

Protective Effect of PineXol® against Amyloid-β-induced Cell Death  

Han, Kyung-Hoon (Research Institute, Seoul Medical Center)
Lee, Seung-Hee (Research Institute, Seoul Medical Center)
Park, Kwang-Sung (Research Institute, Seoul Medical Center)
Song, Kwan-Young (Dept. of Neurosurgery, Seoul Medical Center)
Kim, Jung-Hee (Dept. of Neurosurgery, Seoul Medical Center)
Park, Eun-Kuk (Dept. of Medical Genetics, Ajou University School of Medicine)
Han, Sung-Hee (BK21Plus, College of Health Science, Korea University)
Publication Information
The Korean Journal of Food And Nutrition / v.30, no.6, 2017 , pp. 1279-1285 More about this Journal
Abstract
$Amyloid-{\beta}$ protein ($A{\beta}$) is known to increase free radical production in neuronal cells, leading to cell death by oxidative stress. The purpose of this study was to evaluate the protective effects of $PineXol^{(R)}$ on $A{\beta}_{25-35}$ induced neuronal cell death. Rat pheochromocytoma (PC-12) cells were pre-treated with $100{\mu}g/mL$ of $PineXol^{(R)}$ for 2 h. The cells were exposed to single dose of $30{\mu}M$ $A{\beta}_{25-35}$ for 24 h. Cell death was assessed by a cell count kit-8 (CCK-8) assay, lactate and dehydrogenase (LDH) release assay. An Apoptotic process was analyzed by a protein expression of the Bcl-2 family using western blotting. Cell viability increased in PC-12 cells treated with both $A{\beta}_{25-35}$ and $PineXol^{(R)}$, compared to the control group. $PineXol^{(R)}$ induced a decrease of the Bcl-2 protein expression (p<0.05), while Bax and Sod1 increased (p<0.05), indicating attenuation of $A{\beta}_{25-35}$ induced apoptosis. These results suggest that $PineXol^{(R)}$ may be a good candidate for the prevention of Alzheimer's disease(AD).
Keywords
$PineXol^{(R)}$; Alzheimer's disease; $amyloid-{\beta}$; PC-12 cells; Bcl-2;
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