Browse > Article
http://dx.doi.org/10.9799/ksfan.2016.29.5.760

Quality Characteristics of Pork Skin Collagen with Enzyme Treatments  

Jeon, Ki-Hong (Food Processing Technology Research Center, Korea Food Research Institute)
Hwang, Yoon-Seon (Food Processing Technology Research Center, Korea Food Research Institute)
Kim, Young-Boong (Food Processing Technology Research Center, Korea Food Research Institute)
Choi, Yun-Sang (Food Processing Technology Research Center, Korea Food Research Institute)
Kim, Byoung-Mok (Food Processing Technology Research Center, Korea Food Research Institute)
Kim, Dong-Wook (College of Animal Life Sciences, Kangwon National University)
Jang, Aera (College of Animal Life Sciences, Kangwon National University)
Choi, Jinyoung (Division of Food Science and Culinary Arts, Shinhan University)
Publication Information
The Korean Journal of Food And Nutrition / v.29, no.5, 2016 , pp. 760-766 More about this Journal
Abstract
To increase the collagen recovery rate, bromelain (PB) and a microbial enzyme (PM) were used to treat to pork skin with single agent or combinations. The quality of collagen from the pork skin was evaluated by enzymatic treatments. The highest results for the solid contents and pork skin recovery rate obtained with the microbial-enzyme-bromelain mixtue (PMB) were 13.60% and 18.05% respectively. The result also showed that the color was affected by different types of enzyme treatments. Although PM treatment showed the highest result in the protein content of 251.30 mg/100 g, PMB treatment was the highest in the test of collagen content of 37.73 g/100 g among the treatments. However bands of the pork skin were detected widely at 130 kDa and 170 kDa ranges in SDS-PAGE. The band of PB treatment showed at the range of below 17 kDa, changed into a smaller molecular weight. The collagen content test of the pork skin by the treatments, collagen contents with combination treatment of pork skin with PMB (0.5%) resulted the highest in 43.76 g/100 g. Also the fat content at the above treatment was reduced to 11.12% compared to the other treatments. With these results of this experiment, we conclude that the enzymatic treatments were effective for the processing property of pork skin like enhancing the yield of collagen.
Keywords
pork skin; collagen; bromelain; microbial enzyme; processing property;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Kolar K. 1990. Colorimetric determination of hydroxyproline as measure of collagen content in meat and meat products: NMK collaborative study. J Assoc Off Anal Chem 73:54-57
2 Kwon MC, Syed AQ, Kim HS, Ahn JH, Cho NH, Lee HY. 2008. UV protection and whitening effects of collagen isolated from outer layer of squid Todarodes pacificus. J Kor Fish Soc 41:7-12
3 Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685   DOI
4 Mizuta S, Hwang J, Yoshinaka R. 2002. Molecular species of collagen from wing muscle of skate (Raja kenojei). Food Chem 76:53-58   DOI
5 Mizuta S, Yoshinaka R, Sato M, Sakaguchi M. 1994. Isolation and partial characterization of two distinct types of collagen in the muscle and skin of the squid Todarodes pacificus. Fish Sci 60:467-471   DOI
6 Nagai T, Suzuki N. 2000. Partial characterization of collagen from purple sea urchin (Anthocidaris crassispina) test. Int J Food Sci Technol 35:497-501   DOI
7 Yang SJ, Hong JH. 2012. Extraction and physiochemical properties of collagen from squid (Todarodes pacificus) skin and Alaska pollack (Theragra chalcogramma) skins. Korean J Food Cookery Sci 28:711-719   DOI
8 Kim DW, Park K, Ha G, Jung JR, Chang O, Ham JS, Jeong SG, Park BY, Song J, Jang A. 2013. Anti-oxidative and neuroprotective activities of pig skin gelatin hydrolysates. Korean J Food Sci An 33:258-267   DOI
9 Cho YJ, Seo JE, Kim YJ, Lee NH, Hong SP, Kim YH. 2006. Study on the degradation of pigskin collagen using irradiation technique. J Korean Soc Food Sci Nutr 35:588-593   DOI
10 Han CJ, Kang SM. 2008. The effect of collagen supplementation from pork skin on serum collagen, serum sex steroid hormone, serum lipid and skin crack in Korean middle-aged women. Korean J Community Nutrition 13:912-921
11 Kim HK. 2013. Efficient extraction technique of fish skin collagen and pork cardiac protein by ultrasonic treatment. MS Thesis, Korea Univ. Seoul, Korea
12 Kim HS, Seong JH, Lee YG, Xie CL, Choi WS, Kim SH, Yoon HD. 2009a. Effect of low-molecular-weight collagen peptide extract isolated from scales of the flathead mullet (Mugil cephalus) on lipid metabolism in hyperlipidemic rats. Korean J Food Preserv 16:938-945
13 Kim JW, Kim DK, Park JS, Lee YK, Beik KY. Kim SD. 2009b. Antioxidant and antimicrobial activities of shark collagens and inhibitory actions on elastase and tyrosinase. Korean J Food Preserv 16:419-426
14 Kimura S, Omura Y, Ishida M, Shirai H. 1993. Molecular characterization of fibrillar collagen from the body wall of starfish Asterias amurensis. Comp Biochem Physiol 104:663-668