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http://dx.doi.org/10.9799/ksfan.2015.28.6.956

Effect of Lactic Acid Bacteria Powder on Loperamide-induced Constipation in Rat  

Kim, Eun Young (Dept. of Public Health Sciences, Graduate School, Korea University)
Jo, Kyungae (Dept. of Public Health Sciences, Graduate School, Korea University)
Ahn, So Hyun (Dept. of Public Health Sciences, Graduate School, Korea University)
Park, Sung Sun (Research Center, Chong Kun Dang Healthcare Corporation)
Son, Heung Soo (Dept. of Food Science and Biotechnology, Shin Ansan University)
Han, Sung Hee (Institute for Biomaterials, Korea University)
Publication Information
The Korean Journal of Food And Nutrition / v.28, no.6, 2015 , pp. 956-964 More about this Journal
Abstract
Probiotics is known improve the microenvironment of colon; however, the metagenomic DNA study of its lactic acid bacteria in constipation induced by loperamide is not clearly understood. In the present study, we investigated the reduction of the lactic acid bacteria in case of constipation, in normal and loperamide-induced rat. Lactic acid powder (lactic acid bacteria 19) was prepared from Chong Kun Dang Pharmaceutical Corporation. After 2 weeks of oral administration, the group treated with the higher concentration of lactic acid bacteria ($10^9CFU/mL$ per kg of body weight) following loperamide treatment was the most effective in increasing number, weight, and water content of feces. A similar but significant increase was found in the group treated with lower concentration of lactic acid bacteria ($10^7CFU/mL$ per kg of body weight) after loperamide treatment. The concentrations of acetic acid and propionic acid in feces in the loperamide-induced rat with high concentration lactic acid, were significantly higher than that of others. Furthermore, gastrointestinal transit ratio as well as the length and area of intestinal mucosa were significantly increased after treatment with lactic acid bacteria in loperamide-induced rat. Metagenomics DNA analysis indicated that the microorganism homology in cecum was similar between the groups of normal (NOR) and HIG. Our results show that lactic acid bacteria were effective in improving the constipation.
Keywords
lactic acid bacteria; constipation; loperamide; gastrointestinal transit; H&E staining; metagenomic DNA;
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