Browse > Article
http://dx.doi.org/10.9799/ksfan.2015.28.6.1026

Preparation of High Purity Galacto-Oligosaccharide and Its Prebiotic Activity In Vitro Evaluation  

Hong, Ki Bae (Dept. of Public Health Sciences, Graduate School, Korea University)
Suh, Hyung Joo (Dept. of Public Health Sciences, Graduate School, Korea University)
Kim, Jae Hwan (Neo Cremar Co. Ltd.)
Kwon, Hyuk Kon (Neo Cremar Co. Ltd.)
Park, Chung (LINC Project Group, Daejeon University)
Han, Sung Hee (Institute for Biomaterials, Korea University)
Publication Information
The Korean Journal of Food And Nutrition / v.28, no.6, 2015 , pp. 1026-1032 More about this Journal
Abstract
This study attempted to find an efficient method for the preparation of high-purity galactooligosaccharides (HP-GOS) using ${\beta}$-galactosidase and yeast fermentation. GOS prepared using Lactozym 3000L showed the greatest enhancement in total GOS of the six ${\beta}$-galatosidases tested. GOS alone achieved 51% conversion of initial lactose. GOS production was enhanced by fermentation with commercial yeast (Saccharomyces cerevisiae); its concentration reached 71% after 36h fermentation with 8% yeast. Component sugar analysis with HPLC indicated that HP-GOS fermented with S. cerevisiae showed significantly increased levels of 4'/6'-galactosyllactose and total GOS as well as a significantly decreased glucose level. HP-GOS facilitated the growth of Lactobacillus sp. (L. acidophilus and L. casei) and Bifidobacterium sp. (B. longum and B. bifidum). In sum, high-purity GOS has been successfully produced through both an enzymatic process and yeast fermentation. GOS encourages the growth of bacteria such as Lactobacillus and Bifidobacterium that may be beneficial to human gastrointestinal health.
Keywords
high purity galactooligosaccharide; ${\beta}$-galactosidase; fermentation; Saccharomyces cerevisiae;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Iwasaki K, Nakajima M, Nakao S. 1996. Galacto-oligosaccharide production from lactose by an enzymic batch reaction using $\beta$-galactosidase. Process Biochem 31:69-76   DOI
2 Krisch J, Bencsik O, Papp T, Vagvolgyi C, Tako M. 2012. Characterization of a beta-glucosidase with transgalactosylation capacity from the zygomycete Rhizomucor miehei. Bioresource Technol 114:555-560   DOI
3 Li ZY, Xiao M, Lu LL, Li YM. 2008. Production of nonmonosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells. Process Biochem 43:896-899   DOI
4 Macfarlane GT, Steed H, Macfarlane S. 2008. Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotics. J Appl Microbiol 104:305-344
5 Mahoney RR. 1998. Galactosyl-oligosaccharide formation during lactose hydrolysis: a review. Food Chem 63:147-154   DOI
6 Martinez RCR, Cardarelli HR, Borst W, Albrecht S, Schols H, Gutierrez OP, Maathuis AJH, Franco BDGD, De Martinis ECP, Zoetendal EG, Venema K, Saad SMI, Smidt H. 2013. Effect of galactooligosaccharides and Bifidobacterium animalis Bb-12 on growth of Lactobacillus amylovorus DSM 16698, microbial community structure, and metabolite production in an in vitro colonic model set up with human or pig microbiota. Fems Microbiol Ecol 84:110-123   DOI
7 Nihira T. 2007. Evolution of the oligosaccharide synthesis by glycosynthases. Trends Glycosci Glyc 19:49-50   DOI
8 Onishi N, Yamashiro A, Yokozeki K. 1995. Production of galactooligosaccharide from lactose by Sterigmatomyces elviae Cbs8119. Appl Environ Microb 61:4022-4025
9 Park HY, Kim HJ, Lee JK, Kim D, Oh DK. 2008. Galactooligosaccharide production by a thermostable beta-galactosidase from Sulfolobus solfataricus. World J Microb Biot 24: 1553-1558   DOI
10 Prosky L. 2000. What is dietary fiber? J Aoac Int 83:985-987
11 Shin HJ, Park JM, Yang JW. 1998. Continuous production of galacto-oligosaccharides from lactose by Bullera singularis $\beta$-galactosidase immobilized in chitosan beads. Process Biochem 33:787-792   DOI
12 Shin HJ, Yang JW. 1994. Galacto-oligosaccharide production by $\beta$-galactosidase in hydrophobic organic media. Biotechnol Lett 16:1157-1162   DOI
13 Splechtna B, Nguyen TH, Steinbock M, Kulbe KD, Lorenz W, Haltrich D. 2006. Production of prebiotic galacto-oligosaccharides from lactose using beta-galactosidases from Lactobacillus reuteri. J Agr Food Chem 54:4999-5006   DOI
14 Splechtna B, Petzelbauer I, Baminger U, Haltrich D, Kulbe KD, Nidetzky B. 2001. Production of a lactose-free galacto-oligosaccharide mixture by using selective enzymatic oxidation of lactose into lactobionic acid. Enzyme Microb Tech 29:434-440   DOI
15 Talukder S. 2015. Effect of dietary fiber on properties and acceptance of meat products: a review. Crit Rev Food Sci 55:1005-1011   DOI
16 Torres DPM, Goncalves MP, Teixeira JA, Rodrigues LR. 2010. Galacto-oligosaccharides: Production, properties, applications, and significance as prebiotics. Compr Rev Food Sci 9: 438-454   DOI
17 Trowell H. 1988. Dietary fiber definitions. Am J Clin Nutr 48:1079-1080   DOI
18 Bridiau N, Taboubi S, Marzouki N, Legoy MD, Maugard T. 2006. beta-Galactosidase catalyzed selective galactosylation of aromatic compounds. Biotechnol Progr 22:326-330   DOI
19 Gonzalez R, Klaassens ES, Malinen E, de Vos WM, Vaughan EE. 2008. Differential transcriptional response of Bifidobacterium longum to human milk, formula milk, and galactooligosaccharide. Appl Environ Microb 74:4686-4694   DOI
20 Gursoy N. 2011. The effects of Bifidobacterium lactis and galactooligosaccharide (GOS) on ileum and distal colon motility: In vitro study. Afr J Microbiol Res 5:5877-5881
21 Ito M, Deguchi Y, Miyamori A, Matsumoto K, Kikuchi H, Matsumoto K, Kobayashi Y, Yajima T, Kan T. 1990. Effects of administration of galactooligosaccharides on the human faecal microflora, stool weight and abdominal sensation. Microbial Ecology & Health and Disease 3:285-292   DOI
22 Zhang S, Tang WZ, Jiang LL, Hou YM, Yang F, Chen WF, Li XZ. 2015. Elicitor activity of algino-oligosaccharide and its potential application in protection of rice plant (Oryza saliva L.) against Magnaporthe grisea. Biotechnol Biotec Eq 29:646-652   DOI