Browse > Article
http://dx.doi.org/10.9799/ksfan.2013.26.3.601

Physicochemical Properties of Depolymerized Barley β-Glucan by Alkali Hydrolysis  

Lee, Sang-Hoon (Dept. of Food Science and Technology, Chungbuk National University)
Jang, Gwi-Yeong (Dept. of Food Science and Technology, Chungbuk National University)
Kim, Kee-Jong (Division of Rice and Winter Cereal Crop, National Institute of Crop Science)
Lee, Mi-Ja (Division of Rice and Winter Cereal Crop, National Institute of Crop Science)
Kim, Tae-Jip (Dept. of Food Science and Technology, Chungbuk National University)
Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
Publication Information
The Korean Journal of Food And Nutrition / v.26, no.3, 2013 , pp. 601-607 More about this Journal
Abstract
This study was performed to investigate the changes of total and soluble ${\beta}$-glucan contents, purity and physicochemical characteristics of alkali hydrolyzed barley varieties: Saessalbori (SSB), Saechalssalbori (SCSB) and Hinchalssalbori (HCSB). The barleys were hydrolyzed at different concentrations of sodium hydroxide (0.2~1.0 N) for 12 hours. Total ${\beta}$-glucan contents of raw SSB, SCSB and HCSB were 8.40, 7.77 and 8.28%, and soluble ${\beta}$-glucan contents were 4.80, 4.16 and 4.61%, respectively. The total ${\beta}$-glucan contents after alkali hydrolyzed at 1.0 N NaOH were 7.54, 6.89 and 7.54%, also soluble ${\beta}$-glucan contents were 4.82, 4.30 and 4.55%, respectively. The degree of purity of soluble ${\beta}$-glucan in SSB, SCSB, and HCSB were 35.79, 30.91 and 33.90%, respectively. They were increased to 74.02, 75.28 and 81.41% after hydrolyzed at 1.0 N NaOH, respectively. The molecular weight and viscosity of soluble ${\beta}$-glucan solutions were decreased as sodium hydroxide concentration was increased. The re-solubility of raw barley ${\beta}$-glucan was about 50%; however, it was increased to approximately 87% as sodium hydroxide concentration was increased.
Keywords
barley; ${\beta}$-glucan; alkali hydrolysis; depolymerization; physicochemical properties;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Gomez C, Navarro A, Manzanares P, Horta A, Carbonell JV. 1997. Physical and structural properties of barley (1${\rightarrow}$3)(1${\rightarrow}$ 4)-${\beta}$-D-glucan. I. Determination of molecular weight and macromolecular radius by light scattering. Carbohydrate Polym 32:7-15   DOI   ScienceOn
2 Grimm A, Kruger E, Burchard W. 1995. Solution properties of ${\beta}$-D-(1,3)(1,4)-glucan isolated from beer. Carbohydrate Polym 27:205-214   DOI   ScienceOn
3 Hasegawa M, Isogai A, Onabe F. 1993. Preparation of low molecular weight chitosan using phosphoric acid. Carbohydrate Polym 20:279-283   DOI   ScienceOn
4 He Y, Pang Y, Liu Y, Li X, Wang K. 2008. Physicochemical characterization of rice straw pretreated with sodium hydroxide in the solid state for enhancing biogas production. Energy & Fuels 22:2775-2781   DOI   ScienceOn
5 Jeon YI, Kim SK. 2000. Production of chitooligosaccharides using an ultrafiltration membrane reactor and their antibacterial activity. Carbohydrate Polym 41:133-141   DOI   ScienceOn
6 Jeong HS, Kang TS, Park HJ, Jung IS, Lee HY. 2004. Characteristics of viscosity and components of soluble extract in oats. Food Eng Prog 8:40-46
7 Jung MH, Youn KS. 2012. Preparation and physicochemical characteristics of octenyl succinated rice starches based on amylose content. Korean J Food Sci Technol 44:577-582   과학기술학회마을   DOI   ScienceOn
8 Kim JT, Noh WS. 1992. The retrogradation and swelling power of modified potato starches. J Korean Soc Agric Chem 35:404-409   과학기술학회마을
9 Varum KM, Simidsrod O. 1988. Partial chemical and physical characterisation of (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-glucans form oat (Avena sativa L.) Aleurone. Carbohydrate Polym 9:103-117   DOI   ScienceOn
10 Wood PJ, Paton D, Siddiqui IR. 1977. Determination of ${\beta}$-glucan in oats and barley. Cereal Chem 54:524-533
11 Campbell GL, Bedford MR. 1992. Enzyme applications for monogastric feeds: A review. Can J Anim Sci 72:449-466   DOI
12 Beer MU, Wood PJ, Weisz J. 1997. Molecular weight distribution and (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-D-glucan content of consecutive extracts of various oat and barley cultivars. Cereal Chem 74:476-480   DOI   ScienceOn
13 Bhatty RS. 1995. Laboratory and pilot plant extraction and purification of ${\beta}$-glucans from hull-less barley and oat bran. J Cereal Sci 22:163-170   DOI   ScienceOn
14 Bourne DT, Wheeler RE. 1984. Environmental and varietal differences in total beta-glucan contents of barley and the effectiveness of its breakdown under different malting conditions. J Inst Brew 90:306-310   DOI
15 Burkus Z, Temelli F. 1998. Effect of extraction conditions on yield, composition and viscosity stability of barley ${\beta}$-glucan gum. Cereal Chem 75:805-809   DOI
16 Byun EH, Kim JH, Sung NY, Choi JI, Lim ST, Kim KH, Yool HS, Byun MW, Lee JW. 2008. Effects of gamma irradiation on the physical and structural properties of ${\beta}$-glucan. Radiat Phys Chem 77:781-786   DOI   ScienceOn
17 Carr JM, Glatter S, Jeraci JL, Lweis BA. 1990. Enzymic determination of ${\beta}$-glucan in cereal-based food products. Cereal Chem 67:226-229
18 Choi HD, Seog HM, Choi IW, Park YK, Lee CH, Shin KS. 2004. Molecular structure of ${\beta}$-glucan isolated from nonwaxy and waxy barley. Food Sci Biotechnol 13:744-748
19 Dawkins NL, Nnanna IA. 1995. Studies on oat gum [(1-3,1-4)-${\beta}$-D-glucan]: composition, molecular weight estimation and rheological properties. Food Hydrocol 9:1-7   DOI   ScienceOn
20 Forrest IS, Wainwright T. 1977. The mode of binding of ${\beta}$-glucans extracted from barley at different temperatures. Carbohydr Res 83:279-286
21 Kim SR, Choi HD, Seog HM, Kim SS, Lee YT. 1999. Physicochemical characteristics of ${\beta}$-glucan isolated from barley. Korean J Food Sci Technol 31:1164-1170   과학기술학회마을
22 Klopfenstein CF. 1988. The role of cereal ${\beta}$-glucans in nutrition and health. Cereal Foods World 33:865-869
23 Roubroeks JP, Andersson R, Mastromauro DI, Christensen BE, Aman P. 2001. Molecular weight, structure and shape of oat (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-D-glucan fractions obtained by enzymatic degradation with (1${\rightarrow}$4)-${\beta}$-D-glucan-4-glucanohydrolase from Trichoderma reesi. Carbohydrate Polym 46:275-285   DOI   ScienceOn
24 Lee YT. 1996. ${\beta}$-Glucans in barley and oats and their changes in solubility by processing. Agric Chem Biotechnol 39:482-487
25 McCleary BV, Codd R. 1991. Measurement of (1-3),(1-4)-${\beta}$ -D-glucan in barley and oats: a streamlined enzymic procedure. J Sci Food Agric 55:303-312   DOI
26 McCleary BV, Glennie-Holmes M. 1985. Enzymatic quantification of (1${\rightarrow}$3)(1${\rightarrow}$4)-${\beta}$-D-glucan in barley and malt. J Inst Brew 91:285-295   DOI
27 Thomas DJ, Atwell WA. 1998. Eagan Press Handbook Series: Starches. Eagan Press, St. Paul, Minnesota, MN, USA
28 Aman P, Graham H. 1987. Analysis of total and insoluble mixed-linked (1${\rightarrow}$3),(1${\rightarrow}$4)-${\beta}$-glucans in barley and oats. J Agric Food Chem 35:704-709   DOI
29 Bae IY, Lee SY, Kim SM, Lee HG. 2009. Effect of partially hydrolyzed oat ${\beta}$-glucan on the weight gain and lipid profile of mice. Food Hydrocol 23:2013-2021
30 Bamforth CW. 1985. Biochemical approaches to beer quality. J Inst Brew 91:154-160   DOI
31 Woodward JR, Fincher GB, Stone BA. 1983. Water-soluble (1${\rightarrow}$ 3),(1${\rightarrow}$4)-${\beta}$-D-glucans from barley (Hordeum vulgare) endosperm. II. Fine structure. Carbohydrate Polym 3:207-225   DOI   ScienceOn