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http://dx.doi.org/10.9799/ksfan.2012.25.2.246

Enhancement of the Anti-hyperglycemic and Antioxidant Activities of Five Selected Beans by the Germination Process  

Cho, Cha-Young (Dept. of Food and Nutrition, Hannam University)
Choi, Hwang-Yong (Dept. of Food and Nutrition, Hannam University)
Jo, Sung-Hoon (Dept. of Food and Nutrition, Hannam University)
Ha, Kyoung-Soo (Dept. of Food and Nutrition, Hannam University)
Chung, Ji-Sang (Erom R&D Center)
Jang, Hae-Dong (Dept. of Food and Nutrition, Hannam University)
Kwon, Young-In (Dept. of Food and Nutrition, Hannam University)
Publication Information
The Korean Journal of Food And Nutrition / v.25, no.2, 2012 , pp. 246-252 More about this Journal
Abstract
After a mixed carbohydrate diet, inhibition of ${\alpha}$-amylase and ${\alpha}$-glucosidase involved in the digestion and absorption of carbohydrates can significantly decrease the postprandial increase of blood glucose level. In the course of screening these useful enzyme inhibitors, we selected five kinds of bean, using an in-vitro enzyme inhibition assay method. To evaluate the effect of germination process on the functionality of the bean, we investigated the inhibitory activities of the water extracts of non-germinated bean and germinated bean against ${\alpha}$-amylase and ${\alpha}$-glucosidase, relevant to postprandial hyperglycemia. We also investigated the oxygen radical absorbance capacity(ORAC), total phenolics content, and postprandial blood glucose lowering effect in rats(Sprague-Dawley rat model). Most germinated beans showed significantly higher ${\alpha}$-glucosidase inhibitory activity, compared with non-germinated beans. Among germinated beans, Glycine max had the highest ${\alpha}$-glucosidase inhibitory activity(53.3%). The water extract of germinated Phaseolus vulgaris L. had the highest ${\alpha}$-amylase inhibitory activity(95.1%), followed by Glycine max(58.7%), and Glycine max L. Merr(54.1%). Furthermore, the five germinated beans also showed high antioxidant activities in ORAC assay. Results suggested that the germination process may improve and enhance the anti-hyperglycemia potential and antioxidant activity of the bean.
Keywords
germination; bean; ${\alpha}$-glucosidase; ${\alpha}$-amylase; ORAC;
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