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http://dx.doi.org/10.9799/ksfan.2011.24.1.065

Effects of a Corn Extract on Mouse Splenocyte and Cytokine Production by Peritoneal Macrophages  

Ryu, Hye-Sook (Dept. of Food and Nutrition, College of Health Sciences, Sangji University)
Publication Information
The Korean Journal of Food And Nutrition / v.24, no.1, 2011 , pp. 65-70 More about this Journal
Abstract
This study was performed to investigate the in vitro effect of a corn water extract on immune function. Splenocyte proliferation was determined by the MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl terazolium bromide) assay after preparing asingle cell suspension. Production of macrophage-secreted interleukin(IL)-$1{\beta}$, IL-6, and interferon(IFN)-${\gamma}$, was detected by ELISA using a cytokine assay kit. After a 48-hr incubation with mitogens(ConA or lipopolysaccharide), mice splenocyte proliferation increased with the addition of a corn water extract supplement at 10, 50, 100, 250, 500, or $1,000\;{\mu}g/m\ell$. Production of IL-$1{\beta}$, IL-6, and IFN-${\gamma}$ increased in treatments supplemented with the corn water extract. In an in vitro study, splenocyte proliferation increased when $50\sim1,000\;{\mu}\ell/m\ell$ corn water extract was added. In an ex vivo experiment, the highest production of cytokines by activated peritoneal macrophages was observed in mice orally administered 500 mg/kg body weight/day.
Keywords
splenocyte proliferation; corn; cytokine; IL-$1{\beta}$; IL-6; IFN-${\gamma}$; immunity;
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Times Cited By KSCI : 4  (Citation Analysis)
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