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Acute Oral Toxicity of Chitooligosaccharides in Mice  

박헌국 (동남보건대학 식품영양과)
Publication Information
The Korean Journal of Food And Nutrition / v.16, no.4, 2003 , pp. 437-443 More about this Journal
Abstract
Chitooligosaccharides were prepared by enzymatic hydrolyzing of crab shell chitosan. Low molecular weight chitooligosaccharides(LMW-chitooligosaccharides), 64.3% of which was composed of trimer, tetramer, and pentamer, was obtained by hydrolyzing chitosan with the chitosanase originated Bacillus pumilus BN-262. High molecular weight chitooligosaccharides(HMW-chitooligosaccharides), 49.3% of which was composed of chitooligosaccharides over heptamer, was obtained by hydrolyzing chitosan with the cellulase originated Trichoderma viride. Acute oral toxicity of chitooligosaccharides were tested in mice. Chitooligosaccharides did not have any toxic effect in mice and oral LD$\_$50/ value of chitooligosaccharides was over 5.0g/kg in mice.
Keywords
chitooligosaccharides; acute oral toxicity; mice;
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1 송병춘, 맹원재 : 현대인의 식생활과 건강, 건국대학교출판부, 서울, p. 67-82(1996)
2 박현서, 이영순, 구성자, 한명주, 조여원 : 식생활과 건강, 효일문화사, 서울, P. 239-253(1997)
3 石倉俊治 : キチソとキトサソ, 月刊ドケミル, 1993-6, 62-64(1993)
4 Horowitz, S.T., Roreman, S. and Blumenthal, H.J. : The preparation of glucosamine oligosaccharide - I. Separation, J. Am. Chem. Soc., 79, 5046-5049 (1957)   DOI
5 Izumi, M. and Ohtakara, A. : Preparation of D-glucosamine oligosaccharides by the enzymatic hydrolysis of chitosan, Agri. Biol. Chem., 51(4), 1189-1191(1987)   DOI
6 Tokoro, A., Tatewaki, N., Suzuki, K., Mikami, T. and Suzuki, S. : Growth-Inhibitory effect of hexa-N-acety-lchitohexaose and chitohexaose against Meth-A solid tumor, Chem. Pharm. Bull., 36(2), 784-790(1988)   DOI   ScienceOn
7 欠吹念 : 最後のべイオマスキチソキトサソ, 枝報堂出版, p.1(1988)
8 전유진,김세권 : 키토산 및 올리고당의 랫드에 대한 급성경구독성, 한국키틴키토산화회지, 4(2), 115-120(1999)
9 흥상필, 김동수 : Trichoderma viride 및 Trichoderma reesei 유래 Cellulase의 키토산 분해 특성, 한국식품과학학회, 30(2), 245-252(1998)
10 福井春雄, 正田敏幸, 藤原公, 村岡高志, 次田隆志 : キチ ソ.キトサソのによゐ作物の生長促進效果 第2報 各種 作物ヘの栽培適用性, 日作四國支紀, 26, 9-16(1989)
11 戶倉淸一 : キチソおよびキトサソの免疫賦活性 月刊フ-ドケミカル, 1995-2, 19-24(1995)
12 김순영 : Aspergillus fumigatus KH-94로부터 분리한 Chi-tosanase의 특성과 된장중 Chitooligosaccharide의 면역분석법 개발, 서울대학교 박사학위논문(1997)
13 田口智子,加談秀夫,奧円拓道,多壽良稀:日本農藝化學 會誌67(大會講演 要旨集), P.315(1993)
14 Muraki, E., Yaku, F. and K.ojima, H. : Preparation and crystallization of D-glucosamine oligosaccharides with dp. 6-8 Carbohvdrate Research 239 227-237(1933)
15 Suzuki, S., Okawa, Y., Okura, Y., Hashimoto, K. and Suzuki, M. : in Chitin and Chitosan Proceedings of the Second Intemational Conference on Chitin/Chitosan, ed. by Hirano, S. and Tokura, S. The Japanese Society of Chitin and Chitosan, Tottori Univ., Tottori, p.210(1982)
16 박헌국 : Chitooligosaccharides의 항균성. 한국식품영양학회지, 14(6), 579-584(2001)
17 Maezaki, Y., Tsuji, K., Nakagawa, Y., Kawai, Y., Akimoto, M., Tsugita, T., T祉ewa, W., Terada, A., Hara, T. and Mitsuoka, T. : Hypocholesterolemic effect of chitosan in adult males, Biosci. Biotech. Biochem., 57(9), 1439-1444(1993)   DOI
18 福井春雄, 藤原公, 村間高志, 次田隆志 : キチソ.キトサ ソのによる作物の生長促進效果 第1報 生長促進とその 作用性, 日作四國支紀, 26, 1-8(1989)
19 坂井和男, 南條文雄, 雅氷泰市 : キチソ.キトサソオリ ゴ糖の生産h利用, 澱粉科興 37(2), 79-86(1990)
20 Uchida, Y., Izumi, M. and Ohtakara, A. : Preparation of chitosan oligomers with purified chitosanase and its application, Annual Review of Japanese Society for Chitin and Chitosan, pp. 93-102(1988)
21 Zbinden, G. and Flury-Roversi, M. Significance ofthe LDso test for toxicological evaluation of chemical substance, Arch. Toxicol., 47, 77-99(1981)   DOI   ScienceOn
22 內田泰 :キチソ.キトサソおよび關連化合物の抗菌性 とその 応用, 化學工業 1991-10, 37-43 (1991)
23 Aiba, S. : Studies on chitosan: 4. Lysozymic hydrolysis of partially N-acetylated chitosan, Int. J. Biol. Macrornol, 14, 225-228(1992)   DOI   PUBMED   ScienceOn
24 坂井和男 : キチソ.キトサソオリゴ糖のと開發と現狀,別 冊フ-ドケミカル-I, 食品化學新聞社, pp.106-111(1987)
25 戶倉淸一 : キチソ,キトサソの生理活性について, 別冊フ-ドケミカル-I, 食品化學新聞社, pp. 5-11(1987)
26 식품의약품안전청 : 의약품 등의 독성시험 기준.식품의약품안전청 고시 제 1998-56호. 1127(1998)
27 內田泰 :キチソキトサソの抗齋性,フ-ドケミカル, 1988-2, 22-29 (1988)
28 Hadwiger, L.A., Fristensky, B. and Riggleman, R.C. : In Chitin, chitosan and related enzymes, Academic Press, Orlando, P.291(1984)