Extraction of Freeze Dried Young Antler by Water and Protease

The freeze dried young antler was extracted by water and proteases. In case of water extraction, the extraction rate was highest when it was reacted in 5% of concentration for 6 hours at 50$^{\circ}C$. The result of HPLC analysis of extract shows that high molecular peak in water extract was transformed into low molecular polk by proteases. The rate of low molecular peak was highest when bacteria protease was used, and its second highest rate was pepsin, but the effect of papain on it was low, The extraction rate of young antler reacted for 5 hours was 33.4%(absorbance 13.25 at 280nm) of bacteria protease, 22.4%(absorbance 10.06) of papain, and 30.2% (absorbance 11.34) of pepsin. The young antler was boiled for 30min and it was reacted by proteases for 5 hours at 50$^{\circ}C$. The extraction rate of it was 47,6%(absorbance 12,54) of bacteria protease, and 26,4%(absorbance 7,48) of papain, and 45.6%(absorbance 7.23) of pepsin, In protein content, water extract was 52,1%, bacteria protease extract was 37.8%, and in amino acid content, water extract was 16.3%, bacteria protease extract was 31.96%, in ash content, water extract was 8.8%, bacteria protease extract was 5.6% by dry base. In mineral content, water extract contains 3.6% of Ca, 8.6% of P, 0.01% of Mg, 1.4 % of Na, 0.02 % of F, and bacteria protease extract contains 2.5% of Ca, 11.8% of P, 0.046 % of Mg, 2.1 % of Na, 0.018 % of F by dry base.

물과 단백질 가수분해 효소에 의한 동결건조 녹용의 추출

동결건조 녹용을 물과 단백질 가수분해 효소로 추출하였다. 동결건조녹용을 물추출하는 경우, 추출효율은 5% 농도로 5$0^{\circ}C$에서 6시간 추출하는 것이 가장 높았다. 추출물을 HPLC 분석한 결과, 물추출액에 존재하던 고분자 단백질은 단백질 가수분해 효소에 의해 저분자화되었다. 저분자화율은 세균 protease를 사용한 경우가 가장 높고 다음 pepsin인데, papain의 효과는 적었다. 생녹용에 단백질 가수분해 효소를 5시간 작용시켜서 얻은 추출액의 추출율은 세균 protease는 33.4% (280nm의 흡광도 13.25), papain은 22.4%(흡광도 10.06), pepsin은 30.2%(흡광도 11.34)였다. 30분 동안 끓인 녹용에 단백질 가수분해 효소를 5시간 작용시킨 용액의 추출율은 세균 protease는 47.6%(흡광도 12.54), papain은 26.4%(흡광도 7.48), pepsin은 45.6%(흡광도 7.23)였다. 단백질함량은 물추출액은 52.1%, 세균 protease 추출액은 37.8%였고, 아미노산 함량은 물추출액은 16.3%, 세균 protease 추출액은 31.9%였다. 회분함량은 물추출액은 8.8%, 세균 protease 추출액은 5.6%였다. 무기물 함량은 물추출액의 경우 Ca 3.6%, P 8.6%, Mg0.01%, Na 1.4%, F 0.02%, 세균 pretense의 경우 Ca 2.5%, P 11.8%, Mg 0.046%, Na 2.1%, F 0.018%였다.