Browse > Article

Studies on the Hydrolysis of Seaweed using Microorganisms and Its Application III. Isolation of Seaweed Hydrolytic Strain from Microfloras in Decayed Pine Tree  

김해섭 (여수대학교 식품공ㆍ영양학부)
최옥수 (순천제일대학 식생활부)
강동수 (여수대학교 식품공ㆍ영양학부)
김지만 (여수대학교 식품공ㆍ영양학부)
김귀식 (여수대학교 식품공ㆍ영양학부)
배태진 (여수대학교 식품공ㆍ영양학부)
Publication Information
The Korean Journal of Food And Nutrition / v.16, no.4, 2003 , pp. 289-295 More about this Journal
Abstract
This is a part of study on the hydrolysis of seaweed using microorganisms. A microflora sample obtained from a decayed pine tree was purified by pure culture of 4 times. As the result, 16 isolated strains were obtained from the microflora sample and then each strain was incubated in a liquid medium with sea tangle powder for 3 weeks. Ratios of reduced sugar to total sugar of 08A211, 08C221 and 08B121 strains were highest. Accordingly, these three strains were incubated in 3 different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week. Cell growth and ratios of reduced sugar to total sugar was highest for 08B121 in all the liquid media.
Keywords
seaweed; hydrolysis; microflora; sea tangle; sea mustard;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Yang, J.S. and Lee, S.R : Effects of ionizing radiation on the extraction yield and viscosity of alginate, Korean J. Food Sci. Technol., 9, 194-198(1977)
2 Sawabe, T., Ezura, Y and Kimura, T. : Characterization of an alginolytic marine bacterium from decaying Rishirikombu Laminariajaponica var. ochotensis, Nippon Suisna Gakkaishi, 58, 141-145(1992)   DOI
3 Sawabe, T., Ezura, Y. and Kimura, T. : Purification and characterization of an alginate lyase from marine Alteromonas sp., Nippon Suisan Gakkaishi, 58, 521-527(1992)   DOI
4 Ng, T.K. and Zeikus, J.G. : Urification and characterization of an endoglucanase(1,4-beta-D-glucan glucanohydrolase) from Clostridium thermocellum, Biochem. J, 199, 341(1981)   DOI
5 Matano, Y., Park, J.S., Goldstein, M.A and Doi, R.H. : Cellulose promotes extracellular assembly of Clostridium cellulovorans cellulosomes, J. Bacteriol., 176, 6952(1994)   DOI
6 Berg, B., Hofsten, B.V. and Pettersson, G. ; Growth and cellulase formation by Cellvibrio fulvus, J. Appl. Bacteriol., 35, 201(1972)   DOI
7 Chung, D.H. : Studies on cellulolytic enzymes produced by Chaetomium globosum. Part 1. Properties of crude cellulolytic enzymes, J. Korean Agricultural ChemicalSociety, 10, 23 -31(1968)
8 Kim, H.S. and Bae, T.J. : Studies on the hydrolysis of seaweed using microorganisms and its application, II. Screening of microfloras involved in hydrolysis of seaweed tenella, seaweed fusiforme and green laver, Korean J Food & Nutr., 15, 257 - 266(2002)
9 Yonemoto, Y., Murata, K., Kimura, A, Yamaguchi, H. and Okayama, K. : Bacterial alginate lyase; characterization of alginate lyase-producing bacteria and purification of the enzyme, J ofFerm. & Bioengineering, 72, 152-157(1991)   DOI   ScienceOn
10 Boyd, J. and Turvey, J.R. : Structural studies of alginic acid, using a bacterial poly-a-L-guluronate lyase, Carbohydrate Research, 66, 187-194(1978)   DOI   ScienceOn
11 Haug, A, Larsen, B. and Smidsrod, O. : Uronic acid sequence in alginate from different sources, Carbohydrate Research, 32, 217 -225(1974)   DOI   ScienceOn
12 Lee, S.R, Cho, H.O. and Park, S.K. : Extraction yield and quality attributes of agar from domestic seaweeds according to various pretreatment, Koran J. Food Sci. Technol., 7,109-114(1975)
13 Kitamikado, M., Tseng, C.H., Aoki, T., Yamaguchi, K. and Araki, T. : Isolation of bacteria capable of producing alginate-degrading enzyme from natural environment, Nipoon Suisan Gakkaishi, 55, 709-713(1989)   DOI
14 Kim, D.S. and Park, Y.H. : Uronic acid composition, Block structure and some related properties of alginic acid (1) Uronic acid composition of alginic acid from ecklonia cava, J Korean Fish. Soc., 17, 391-397(1984)
15 Yoshikawa, T., Suzuki H. and Nisizawa, K. : Biogenesis of multiple cellulase components of Pseudomonas jluorescens var. cellulosa. I. Effects of culture conditions on the multiplicity of cellulase, J. Biochem.(Tokyo), 75, 531(1974)   DOI
16 Kaneko, Y., Yonemoto, Y., Okayama, K., Kimura, A. and Murata, K. : Bacterial alginate lyase properties of the enzyme formed in a mixed culture bacteria isolated from soil, J. of Fermentation & Bioengineering, 70, 147-149(1990)   DOI   ScienceOn
17 Ando, Y. and Inoue, K. : Decomposition of alginic acid by microorganisms- V . On the modes of action of two alginases, Bull. of Jap. Soc. Sci. Fish., 31, 552-557(1965)   DOI
18 Yamane, K, Yoshikawa, T., Suzuki, H. and Nisizawa, K.: Localization of cellulase components in Pseudomonas fluorescens var. cellulosa, J. Biochem. (Tokyo), 69, 771(1971)   DOI
19 Joo, D.S., Lee, J.S, Cho, S.Y., Shin, S.J. and Lee, E.H. : Changes in functional properties of alginic acid by enzymatic degradation, Korean J. Food Sci. Technol., 27, 86-91(1995)
20 Allcock, E.R. and Woods, D.R. : Carboxymethyl cellulase and cellobiase production by Clostridium acetobutylicum in an industrial fermentation medium, Appl. Environ. Microbiol.,41, 539(1981)
21 Waksman, S.A. and Allen, M.C. : Decomposition of polyuronides by fungi and bacteria, J. Bacteriol., 28. 213-220(1934)
22 Chang, W.T. and Thayer, D.W. : The cellulase system of a Cytophaga species, Can. J. Microbiol., 23, 1285(1977)   DOI   ScienceOn
23 Oberkotter, L.V. and Rosenbergh, F.A. : Extracellular endobeta-l,4-glucanase in Cellvibrio vulgaris, Appl. Environ. Microbiol., 36, 205(1978)
24 Groleau D, and Yaphe, W. : Enzymatic hydrolysis of agar; purification and characterization of p-neoagarotetraose hydrolase from Pseudomonas atlantica, Can. J Microbiol., 23, 672-679(1977)
25 Joo, D.S., Lee, J.S., Park, J.J., Cho, S.Y., Kim, H.K. and Lee, E.H. : Preparation of oligosaccharides from alginic acid by enzymic hydrolysis, Korean J. Food Sci. Technol., 28, 146-151(1996)
26 Cho, H.O. and Lee, S.R. : Effectiveness of gammairradiation on the extraction of algal polysaccharides. Korean J. Food Sci. Technol., 6(1), 36-41(1974)
27 Kim, H.S. and Bae, T.J. : Studies on the hydrolysis of seaweed using microorganisms and its application, I . Screening of microfloras involved in hydrolysis of sea tangle(Laminariajaponica) and sea mustard(Undaria pinnatifida pinnatifida), J Korean Fish. Soc., 35, 438-444(2002)
28 Davidson, I.W., Sutherland, l.W, and Lawson, C.J. : Purification and properties of an alginate lyase from a marine bacterium, Biochem. J., 159, 707-713(1976)
29 Stewart, B.J. and Leatherwood, J.M. : Derepressed synthesis of cellulase by Cellulomonas, J. Bacteriol., 128, 609(1976)
30 Gilkes, N.R, Langsford, M.L., Kilburn, D.G., Jr. Miller, R.C. and Warren, R.A. : Mode of action and substrate specificities of cellulases from cloned bacterial genes, J. BioI. Chem., 259. 10455(1976)
31 Chung, D.H. : Studies on cellulolytic enzymes produced by Chaetomium globosum. Part 2. Purification of cellulase, J. Korean Agricultural Chemical Society, 12. 33 -41(1969)
32 Chung, S.H. and Park, K.H. : Purification and characterization of plant cell wall lytic enzyme from Fusarium moniliforme, Kor. J. Appl. Microbiol. Biotech., 18. 154158(1990)
33 Cho, H.O. and Lee, S.R. : Effectiveness of gammairradiation of the extraction of algal polysaccharides, Korean J. Food Sci. Technol., 6, 36-41(1974)
34 Kaneko, Y, Yonemoto, Y., Okayama, K., Kimura, A. and Murata, K. : Symbiotic formation of alginate lyase in mixed culture of bacteria isolated from soil, J. Fer. and Bioen., 69,192-194(1990)   DOI   ScienceOn
35 Joo, D.S., Lee, J.S., Park, J.J., Cho, S.Y., Ahn, C.B. and Lee, E.H. : Purification and characterization of the intracellular alginase from Vibrio sp. AL-145, Kor. J. Appl. Microbiol. Biotechnol., 23, 432-438(1995)
36 Boyd, J. and Turvey, J.R. : Isolation of a poly-a-Lguluronate lyase from Klebsiella aerogenes, Carbohydrate Research, 57, 163-171(1977)   DOI   ScienceOn
37 Beguin, P. and Eisen, H. : Purification and partial characterization of three extracellular cellulases from Cellulomonas sp., Eur. J. Biochem., 87, 525(1978)   DOI   ScienceOn
38 Kashiwabara, Y, Hiroshi, S. and Nisizawa, K. : Alginate Iyases of Pseudomonas sp., J. Biochem., 66, 503 - 512(1969)   DOI