Inhibitory Effects of Wax Gourd Extract on Melanin Formation and Acne-forming Bacterial Growth

Lyophilizate of immatured wax gourd extract was 3.1 %, matured wax gourd extract was 1.0%, and its main ingredient was sugar, which accounts for 89.7% in total residue. In matured wax gourd, pectin contents was 4.11 mg/ml, and in immatured wax gourd 4.43 mg/m1. In matured wax gourd sarcocarp, sugar contents was 0.1% of sucrose, 0.32% of glucose, 0.35% of fructose, the first unidentified sugar was 0.06% and the second was 0.04%, and all total 0.87%. In sarcocarp of immatured wax gourd, sucrose was 0.33%, glucose was 1.04%, frutcose was 1.12%, and the first unidentified sugar 0.18%, and the second was 0.l2, which total 2.79%. In matured wax gourd core, pH was 4.64, sarcocarp 4.94, immatured wax gourd core 4,96, sarcocarp 5.40. According to the organic acid analysis, in sarcocarp of matured wax gourd, citric acid of 0.409 was contained, magic acid 0.084, succnic acid 0.048%, in matured wax gourd core, citric acid was 0.648, magic acid 0.127, succinc acid 0.058%, in immatured wax gourd, citric acid 0.023, magic acid 0.219, succinic acid 0.298%, in immutured wax gourd, citric acid was 0.039, malic acid 0.350, succinic 0.224%. Fumaric acid was trace in all cases. Total organic acid in matured wax gourd core was 0.833, immatured wax gourd core was 0.624 and immatured wax gourd sarcocarp was 0.546, matured wax gourd sarcocarp was 0,541%. In inhibition rate to propionibacterium acnes, control was 0(ø, cm), wax gourd that was not heated was 2.6, and wax gourd which was heated was 2.5, concentrated by 1/5 was 1.9, wax gourd by 1/10 was 2.5, freezing dry was 2.3. Wax gourd which not heated on producing melanin in B-16 melanoma cell, the melanins forming unit was 15$\mu$1/m1 in addition of 0.01%, while that as a control was 29$\mu$1/m1.

동아를 이용한 미용식품 및 화장품 개발 1. 멜라닌 생성 억제와 여드름균 억제에 미치는 동아 추출액의 효과

고형분 함량은 어린 동아 착즙액에서는 3.1%, 익은 동아 착즙액에서는 0.1%를 나타냈고, 주성분은 당으로 89.7%를 나타냈다. 펙틴 함량은 익은 동아즙은 4.l1mg/ml, 어린 동아즙은 4.43mg/ml이었다. 당 함량은 익은 동아 육질은 sucrose 0,1%, glucose 0.32%, fructose 0.35%, 미확인당 1이 0.06%, 2가 0.04%로 합계 0.87%를 나타냈다. 어린 동아 육질은 sucrose 0.33%, glucose 1.04%, fructose 1.12%, 미확인당 1이 0.18%, 2가 0.12%로 합계 2.79%를 나타냈다. pH는 익은 동아는 4.64, 육질은 4.94, 어린 동아의 속은 4.96, 육질은 5.40을 나타냈다. 유기산은 익은 동아 육질에는 citric acid 0.409, magic acid 0.084, succnic acid 0.048%, 속에는 citric acid 0.648, malic acid 0.127, succinic acid 0.057%, 어린 동아 육질에는 citric acid 0.028, malic acid 0.219, succinic acid 0.298%, 속에는 citric acid 0.039, malic acid 0.360, succinic acid 0.224% 들어 있다. Fumaric acid는 미미하다. 총량은 익은 동아 속이 0.833, 어린 동아속 0.624, 어린 동아 육질 0.546, 익은 동아 육질 0.541이었다. 여드름균(Propionibacterium acnes)에 대한 항균력은 대조군이 0($\Phi$, cm)인데 반해, 비열처리 동아즙은2.6, 열처리 동아즙은2.5, 1/5 농축한 것은 1.9, 1/10 농축한 것은 2.5, 동결건조한 것은 2.3을 나타냈다. 멜라닌 생성 억제력은 0.01%에서 대조군이 29$\mu$l/m1인 데 반해 열처리 동아즙은 15$\mu$l/ml 을 나타냈고, 비열처리 동아즙은 이보다 약간 높았다. 1/5 농축한 것은 24 $\mu$l/ml, 1/10 농축한 것은 23 $\mu$l/ml, 동결건조한 것은 19 $\mu$l/ml를 나타냈다. 이들 결과에 따라 동아즙을 30% 가하여 나리싱크림과 밀크로숀을 조제하였다.