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http://dx.doi.org/10.5536/KJPS.2014.41.1.15

Production of Specific Egg Yolk Antibodies in Chicken against Recombinant Fowl Adenovirus Fiber 2 Protein  

Jung, Kyung Min (Department of Animal Resources and Science, Dankook University)
Lee, Seong (Department of Animal Resources and Science, Dankook University)
Kim, Jung Woo (Department of Animal Resources and Science, Dankook University)
Publication Information
Korean Journal of Poultry Science / v.41, no.1, 2014 , pp. 15-20 More about this Journal
Abstract
Fowl adenovirus (FAV) is an important cause of several diseases, which result in considerable economic losses to the poultry farm. An outer capsid protein of FAV, fiber 2 is essential for virus growth, assembly or spread. This study was performed to produce about 22 kDa of recombinant fiber 2 protein and to immunize in laying hens to acquire the specific IgY antibody against the recombinant fiber 2. Laying hens were immunized with the recombinant fiber 2 intramuscularly in the breast muscle by injection 4 times at intervals of three weeks. At 12 weeks, serum- and egg yolk-antibody titers of hens against fiber 2 were increased up to 430,000 and 414,000, respectively. The recombinant fiber 2 could be recognized be the anti-His monoclonal antibody. Anti-fiber 2-IgY antibody could recognize the fiber 2 specifically in western blot analysis. These results suggested that the recombinant fiber 2 antigen could be used as an immunogen to elicit IgY antibody against fiber 2 and the anti-fiber 2-IgY could neutralize fowl adenovirus fiber 2 effectively.
Keywords
recombinant FAV fiber 2; IgY; immune reaction;
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Times Cited By KSCI : 1  (Citation Analysis)
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