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http://dx.doi.org/10.5536/KJPS.2013.40.4.299

Primary Culture of Chicken Tracheal Epithelial Cells and Study on Those Characters for Recombinant Virus Infection  

Park, Mi Na (National Institute of Animal Science, RDA)
Jang, Hyun-Jun (National Institute of Animal Science, RDA)
Keum, Dae Ho (National Institute of Animal Science, RDA)
Choi, Jin Ae (National Institute of Animal Science, RDA)
Yoo, Jae Gyu (National Institute of Animal Science, RDA)
Byun, Sung June (National Institute of Animal Science, RDA)
Park, Jong Ju (National Institute of Animal Science, RDA)
Ji, Ju Young (National Institute of Animal Science, RDA)
Lee, Kyung-Tai (National Institute of Animal Science, RDA)
Kim, Tae-Hun (National Institute of Animal Science, RDA)
Lee, Hyun-Jeong (National Institute of Animal Science, RDA)
Publication Information
Korean Journal of Poultry Science / v.40, no.4, 2013 , pp. 299-304 More about this Journal
Abstract
Tracheal epithelial cells (TECs) are an important tool for studies of viral respiratory diseases. Primary TECs have been cultured from human, mouse and hamster. It is also necessary to diagnose viral respiratory disease and reveal infection mechanisms in chicken. In this study, we isolated tracheal epithelial layers from tracheal of 20-day-old chicks and cultured primary TECs from the isolated layers. Ciliated cells which were a typical morphology of TECs were observed in cultured primary TECs and maintained until cell passage 5 (15 to 20 days). When we analyzed expression patterns of epithelial marker genes (retinoic acid responder, FGF-binding protein, virus activating protease (VAP) in TECs compared to immortalized chicken embryonic fibroblast cell line (DF-1), all the marker genes are highly expressed in TECs than in DF-1. When TECs were cultured with 0.1 and 1 MOI of ND virus (rNDV-GFP strain) to test the susceptibility of TECs for ND virus, 12.6% and 48.2% of the incubated TECs were infected respectively. In addition, when DF-1 was incubated with 1 MOI of ND virus, the virus infection rate of DF-1 was three times lower than the virus infection rate of TECs. These data could contribute to study infection mechanisms of viral respiratory diseases and control them in chicken.
Keywords
chicken; tracheal epithelial cell; primary culture; viral respiratory diseases;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Runkel F, Michels M, Franken S, Franz T 2006 Specific expression of annexin A8 in adult murine stratified epithelia. J Mol Hist 37:353-359.   DOI
2 Song Y, Jayaraman S, Yang B, Matthay MA 2001 Role of aquaporin water channels in airway fluid transport, humidification and surface liquid hydration. J Gen Physiol 117: 573-582.   DOI
3 Tobita K, Sugiura A, Enomoto C, Furuyama M 1975 Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells(MDCK) in the presence of trypsin. Med Microbiol Immunol 162:9-14.   DOI   ScienceOn
4 Zaffuto KM, Estevez CN, Afonso CL 2008 Primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses. Avian Pathology 37(1):25-31.   DOI   ScienceOn
5 최강석 이은경 전우진 권준현 양창범 2008 흰뺨검둥오리 (Anas poecilorhyncha)에서 분리된 뉴캣슬병 바이러스의 특성. 대한수의학회지 48(2):153-159.   과학기술학회마을
6 최강석 2010 최근 국내에서 유행하는 뉴캣슬병 바이러스의 특성 고찰. 한국가금학회지 37(1):89-99.
7 Beer HD, Bittner M, Niklaus G, Munding C, Max N, Goppelt A, Werner S 2005 The fibroblast growth factor binding protien is a novel interaction partner of FGF-7, FGF-10 and FGF-22 and regulates FGF activity: Implications for epithelial repair. Oncogene 24:5269-5277.   DOI   ScienceOn
8 Cummiskey JF, Hallum JV, Skinner MS, Leslie GA 1973 Persistent newcastle disease virus infection in embryonic chicken tracheal organ cultures. Infec Immun 8(4):657-664.
9 Gresland L, Niveleau A, Hupper J 1979 Replication cycle of newcastle disease virus in three host cells of different permissiveness. J Gen Virol 45:569-578.   DOI   ScienceOn
10 Ibricevic A, Pekoxz A, Walter MJ, Newby C, Battaile JT, Brown EG, Holtzman MJ, Brody SL 2006 Influenza virus receptor specificity and cell tropism in mouse and human airway epithelial cells. Journal of Virology 80(15):7469-7480.   DOI   ScienceOn
11 Matrosovich MN, Matroshvich TY, Gray T, Roberts NA, Klenk HD 2004 Human and avian influenza viruses target different cell types in cultures of human airway epithelium. PNAS 101(13):4620-4624.   DOI   ScienceOn
12 Newby CM, Rowe RK, Pekosz A 2006 Influenza A virus infection of primary differentiated airway epithelial cell cultures derived from Syrian Golden hamsters. Virology 354:80-90.   DOI   ScienceOn
13 Park MS, Shaw ML, Monoz JJ, Cros JF, Nakaya T, Bouvier N, Palese P, Garcia SA, Basler CF 2003 Newcastle desease virus (NDV)-based assay demonstrates interferon-antagonist activity for the NDV V protein and the nipah V, W, C proteins. Journal of Virology 77(2):1501-1511.   DOI   ScienceOn