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http://dx.doi.org/10.5536/KJPS.2012.39.2.113

Biological Properties of Vero Cell-Adapted Newcastle Disease Virus  

Choi, Kang-Seuk (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Park, Mi-Ja (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kye, Soo-Jeong (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kim, Ji-Ye (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kwon, Jun-Hun (Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Publication Information
Korean Journal of Poultry Science / v.39, no.2, 2012 , pp. 113-120 More about this Journal
Abstract
Newcastle disease virus (NDV) Kr005/V strain was generated through 55 serial passages of NDV Kr005 strain in Vero cells. The Kr005/V virus yielded high infective titers of $10^{7.8}$ $TCID_{50}/mL$ in Vero cells and the infected cells showed cytopathic effects such as marked cell rounding, though less frequent syncytia. The Kr005/V virus was heat-stable and classified into the lentogenic type with a Mean Death Time (MDT) of 120h or greater while the Kr005 strain was heat-labile and velogenic (MDT of 49.6 h). Only the single amino acid substitution (T to S) was observed at position 433 of the HN protein of the Kr005/V strain, whereas no amino acid change was found in the F protein. The Kr005/V input virus correlated well (correlation coefficient $r^2$=0.97) with the Kr005 virus when ten field sera were tested by virus neutralization test. The biological properties and usefulness of Vero cell-adapted Kr005/V virus were discussed.
Keywords
Newcastle disease virus; Vero cells; biological properties; virus neutralizing antibody;
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1 Reddy GS, Srinivasan VA 1992 Use of BHK cell culture-adapted Newcastle disease virus for immunization of chicks. Vaccine 10:164-166.   DOI   ScienceOn
2 Reed LJ, Muench H 1938 A simple method for estimating fifty percent endpoints. Am J Hyg 27:493-497.
3 Romer-Oberdörfer A, Werner O, Veits J, Mebatsion T, Mettenleiter TC 2003 Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J Gen Virol 84:3121-3129.   DOI   ScienceOn
4 Takimoto T, Taylor GL, Connaris HC, Crennell SJ, Portner A 2002 Role of the hemagglutinin-neuraminidase protein in the mechanism of paramyxovirus-cell membrane fusion. J Virol 76:13028-13033.   DOI
5 김재홍 송창선 1992 최근의 닭 뉴캣슬병 만연에 따른 원인과 바이러스의 성장, 진단 및 백신 운용에 관한 고찰. 한국가금학회지 19:65-76.
6 박근식 1979 한국에 있어서 뉴캣슬병 발생의 역학적 조사. 한국가금학회지 6:38-46.
7 최강석 2010 최근 국내에서 유행하는 뉴캣슬병 바이러스의 특성 고찰. 한국가금학회지 37:89-99.
8 Kumanan K, Vijayarani K, Padmanabhan VD 1993 Adaptation of a velogenic Newcastle disease virus isolate to Vero cells. Indian Vet J 70:993-995.
9 Lee YJ, Sung HW, Choi JG, Kim JH, Song CS 2004 Molecular epidemiology of Newcastle disease viruses isolated in South Korea using sequencing of the fusion protein cleavage site region and phylogenetic relationships. Avian Pathol 33:482-491.   DOI   ScienceOn
10 Lee YJ, Sung HW, Choi JG, Lee EK, Yoon Y, Kim JH, Song CS 2008 Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins. J Vet Sci 9:301-308.   DOI   ScienceOn
11 Lomniczi B 1975 Thermostability of Newcastle disease virus strains of different virulence. Arch Virol 47:249-255.   DOI
12 Mayo MA 2002 A summary of taxonomic changes recently approved by ICTV. Arch Virol 147:1655-1663.   DOI   ScienceOn
13 Melanson VR, Iorio RM 2004 Amino acid substitutions in the F-specific domain in the stalk of the Newcastle disease virus HN protein modulate fusion and interfere with its interaction with the F protein. J Virol 78:13053-13061.   DOI   ScienceOn
14 Mohan CM, Dey S, Kumanan K, Manohar BM, Nainar AM 2007 Adaptation of a velogenic Newcastle disease virus to Vero cells: assessing the molecular changes before and after adaptation. Vet Res Commun 31:371-383.   DOI
15 Panda A, Huang Z, Elankumaran S, Rockemann DD, Samal SK 2004 Role of 250 fusion protein cleavage site in the virulence of Newcastle disease virus. Microb Pathog 36:1-10.   DOI   ScienceOn
16 Peeters BP, de Leeuw OS, Koch G, Gielkens AL 1999 Rescue of Newcastle 253 disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a 254 major determinant for virulence. J Virol 73:5001-5009.
17 Bankowski RA 1964 Propagation of Newcastle Disease Virus. pp 231-246. In: Newcastle Disease Virus: An Evolving Pathogen. University Wisconsin Press, Madison.
18 Gravel KA, Morrison TG 2003 Interacting domains of the HN and F proteins of Newcastle disease virus. J Virol 77:11040-11049.   DOI
19 de Leeuw OS, Koch G, Hartog L, Ravenshorst N, Peeters BP 2005 Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein. J Gen Virol 86:1759-1769.   DOI   ScienceOn
20 Fenner F, McAuslan BB, Mims CA, Sambrook J, White DO 1974 The Biology of Animal Viruses. p 40. Academic Press, London.
21 Heminway BR, Yu Y, Galinski MS 1994 Paramyxovirus mediated cell fusion requires co-expression of both the fusion and hemagglutinin-neuraminidase glycoproteins. Virus Res 31:1-16.   DOI   ScienceOn
22 Horvath CM, Paterson RG, Shaughnessy MA, Wood R, Lamb RA 1992 Biological activity of paramyxovirus fusion proteins: factors influencing formation of syncytia. J Virol 66:4564-4569.
23 Huang Z, Panda A, Elankumaran S, Govindarajan D, Rockemann DD, Samal SK 2004 The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism and virulence. J Virol 78:4176-4184.   DOI   ScienceOn
24 Iorio RM, Glickman RL 1992 Fusion mutants of Newcastle disease virus selected with monoclonal antibodies to the hemagglutinin-neuraminidase. J Virol 66:6626-6633.
25 Khattar SK, Yan Y, Panda A, Collins PL, Samal SK 2009 A Y526Q mutation in the Newcastle disease virus HN protein reduces its functional activities and attenuates virus replication and pathogenicity. J virol 83:7779-82.   DOI   ScienceOn
26 Kumanan K, Venkatesan RA 1994 Adaptation of Newcastle disease virus strains to different cell culture. Indian J Comp Microbiol Immunol Infect Dis 15:39-41.
27 Alexander DJ 2003 Newcastle Disease. pp 64-87 In: Diseases of Poultry, 11th edition. Iowa State University Press, Ames, IA.
28 Ahamed T, Hossain KM, Billah MM, Islam KMD, Ahasan MM, Islam ME 2004 Adaptation of Newcastle disease virus (NDV) on Vero cell line. Int J Poult Sci 3: 153-156.   DOI
29 Alexander DJ 1988 Newcastle Disease Diagnosis. pp 147-160. In: Newcastle Disease. Kluwer Academic Publishers, Boston.