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http://dx.doi.org/10.5536/KJPS.2009.36.4.301

Analysis of Lipogenic and Myogenic Gene Expressions in Energy Restricted Broiler Chickens  

Moon, Yang-Soo (Department of Animal Science and Biotechnology, Jinju National University)
Publication Information
Korean Journal of Poultry Science / v.36, no.4, 2009 , pp. 301-309 More about this Journal
Abstract
The present study was conducted to determine the effect of energy restricted (ER) diet on the expressions of lipogenic genes in liver and myogenic genes in muscle tissue of broiler chickens. Energy restriction was accomplished by providing chicks with 70% (ER70) or 85% (ER85) energy level of control diet intake. Energy restricted groups of chickens were restricted for 7 days, starting at 8 days of age. Ad libitum feeding was resumed after the restriction period, and continued through the end of the experiment. The body weight of chickens in the restricted groups gained less during the energy restriction period (P<0.05). The body weight of the ER groups were similar to the control group during the re-alimentation period. However, the body weight of the ER70 group did not catch up with that of the control group by 35 days of age. The energy restrictions during early life of chicks decreased the contents of triglycerides and cholesterol in blood (P<0.05), but those were not different among treatments after re-alimentation. The genes of fatty acid synthase (FAS) and transcription factors including SREBP and PPARγ were down regulated by restriction regimen only in ER70 (P<0.05). However, those genes were not completely recovered after re-alimentation in ER70 group. The RNA expression levels of Myo-D, Myf-5 and myogenin in all treatment groups were decreased by restriction regimen when compared with control group (P<0.05). Myogenin was highly expressed after re-alimentation, but the other genes were not different among groups. These results suggest that ER85 group shows the best growth performance by re-alimentation and the higher muscle differentiation by expressing myogenin.
Keywords
lipogenic genes; myogenic genes; gene expression; energy restriction; broiler;
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