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A Study of the Liposome-Mediated Transgenic Chicken Production  

Byun S. J. (National Livestock Research Institute, Biotechnology Division)
Park C. (National Livestock Research Institute, Biotechnology Division, Department of Animal Biotechnology, Jinju National University)
Yang B. S. (National Livestock Research Institute, Biotechnology Division)
Kim T. Y. (The Catholic University of Korea)
Sohn S. H. (Department of Animal Biotechnology, Jinju National University)
Kim S. H. (Department of Biology, Kyung Hee University)
Jeon I. S. (National Livestock Research Institute, Biotechnology Division)
Publication Information
Korean Journal of Poultry Science / v.31, no.4, 2004 , pp. 293-298 More about this Journal
Abstract
Microinjection of DNA is a general method for generating transgenic animals, but the rate of transgenesis in chickens is very low. So it was carried out to investigate the efficiency of liposome-mediated gene transfer in stage one cell of chicken embryo with GFP expression vector. In order to determine efficiency and duration of the introduced foreign gene, it was microinjected DNA with liposome or naked DNA into the germinal disc of stage one cell or stage-X chicken embryo. Analysis of reporter gene expression in day-4 embryos showed that GFP expression was observed only in the liposome-mediate embryo groups and detectable up to day-8 embryos. The results suggest that stable integration of the introduced gene using liposome is a rare event. Nevertheless the liposome-mediated gene transfer may be a useful method to transfer a foreign gene into the stage one cell of chicken embryos.
Keywords
liposome; microiniection; GFP; chicken embryo;
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