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Antioxidative Effects of Delphinidin under in vitro and Cellular System  

Noh, Jeong-Sook (Department of Food Science and Nutrition, Research Institute of Ecology for the Elderly, Pusan National University)
Cho, Yun-Ju (Department of Food Science and Nutrition, Research Institute of Ecology for the Elderly, Pusan National University)
Kim, Boh-Kyung (Department of Food Science and Nutrition, Research Institute of Ecology for the Elderly, Pusan National University)
Park, Kun-Young (Department of Food Science and Nutrition, Research Institute of Ecology for the Elderly, Pusan National University)
Cho, Eun-Ju (Department of Food Science and Nutrition, Research Institute of Ecology for the Elderly, Pusan National University)
Publication Information
Food Science and Biotechnology / v.18, no.1, 2009 , pp. 167-171 More about this Journal
Abstract
This study examined the antioxidative activity of delphinidin, a kind of anthocyanidin from eggplant. Cellular protective potential from oxidative damage by nitric oxide (NO), superoxide anion ($O_2^-$), and peroxynitrite ($ONOO^-$) using epithelial cell line LLC-PK1 cell as well as in vitro radical scavenging effects were investigated. Delphinidin showed strong in vitro radical scavenging effects against NO, $O_2^-$, and hydroxyl radical (${\cdot}OH$) in dose-dependent manners. In addition, delphinidin increased cell viability in LLC-PK1 cells in a concentration-dependent manner when viability was reduced by $ONOO^-$-induced oxidative damage. To elucidate the protective mechanisms of delphinidin from $ONOO^-$, sodium nitroprusside (SNP), and pyrogallol were also employed to generate NO and $O_2^-$, respectively. The treatment of delphinidin recovered reductions in cell viability caused by SNP and pyrogallol, indicating that delphinidin can attenuate oxidative stress induced by NO and $O_2^-$. The present study suggests that delphinidin is a promising anti oxidative agent.
Keywords
delphinidin; antioxidative activity; peroxynitrite; oxidative stress; LLC-PK1 cell;
Citations & Related Records
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