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Characterization of Lactobacilli with Tannase Activity Isolated from Kimchi  

Kwon, Tae-Yeon (Department of Food Science and Biotechnology, Kyonggi University)
Shim, Sang-Min (Department of Food Science and Biotechnology, Kyonggi University)
Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University)
Publication Information
Food Science and Biotechnology / v.17, no.6, 2008 , pp. 1322-1326 More about this Journal
Abstract
Tannase catalyzes the hydrolysis of gallic acid esters and hydrolysable tannins. Twenty-two Lactobacillus strains with tannase activity were isolated from 7 types of kimchi. A polymerase chain reaction-based assay targeting the recA gene assigned all isolates to either Lactobacillus plantarum or Lactobacillus pentosus. The tannase activities of isolates measured in whole cells and cell-free extracts varied even within each species. The activities of the isolates varied with the assay method, but both methods indicated that isolate LT7 (identified as L. pentosus) showed the highest activity. The results of thin layer chromatography and high performance liquid chromatography, respectively, showed that tannic acid and gallic acid degraded to pyrogallol in resting L. pentosus LT7 cells. Therefore, the putative biochemical pathway for the degradation of tannic acid by L. pentosus implies that tannic acid is hydrolyzed to gallic acid and glucose, with the formed gallic acid being decarboxylated to pyrogallol. This study revealed the possible production of pyrogallol from tannic acid by the resting cell reaction with L. pentosus LT7.
Keywords
tannin; tannase; tannic acid; gallic acid; pyrogallol; Lactobacillus plantarum; Lactobacillus pentosus;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
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1 Boadi DK, Neufeld RJ. Encapsulation of tannase for the hydrolysis of tea tannins. Enzyme Microb. Tech. 28: 590-595 (2001)   DOI   ScienceOn
2 Nishitani Y, Osawa R. A novel colorimetric method to quantify tannase activity of viable bacteria. J. Microbiol. Meth. 54: 281-284 (2003)   DOI   ScienceOn
3 Lee J-H, Kim M, Um S. PCR-based detection and identification of Lactobacillus plantarum, Lactobacillus pentosus, and Lactobacillus paraplantarum in kimchi. Food Sci. Biotechnol. 13: 754-757 (2004)
4 Vaquero I, Marcobal A, Munoz R. Tannase activity by lactic acid bacteria isolated from grape must and wine. Int. J. Food Microbiol. 96: 199-204 (2004)   DOI   ScienceOn
5 Nishitani Y, Sasaki E, Fujisawa T, Osawa R. Genotypic analysis of lactobacilli with a range of tannase activities isolated from human feces and fermented foods. System. Appl. Microbiol. 27: 109-117 (2004)   DOI   ScienceOn
6 Osawa R. Formation of a clear zone on tannin-treated brain heart infusion agar by a Streptococcus sp. isolated from feces of koalas. Appl. Environ. Microb. 56: 829-831 (1990)
7 Sharma S, Gupta MN. Synthesis of antioxidant propyl gallate using tannase from Aspergillus niger van Teighem in nonaquoeous media. Bioorg. Med. Chem. Lett. 13: 395-397 (2003)   DOI   ScienceOn
8 Osawa R, Kuroiso K, Goto S, Shimizu A. Isolation of tannin-degrading lactobacilli from humans and fermented foods. Appl. Environ. Microb. 66: 3093-3097 (2000)   DOI   ScienceOn
9 Alberto MR, Gomez-Cordoves C, Manca de Nadra MC. Metabolism of gallic acid and catechin by Lactobacillus hilgardii from wine. J. Agr. Food Chem. 52: 6465-6469 (2004)   DOI   ScienceOn
10 Torriani S, Felis GE, Dellaglio F. Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers. Appl. Environ. Microb. 67: 3450-3454 (2001)   DOI   ScienceOn
11 Lee KH, Park JY, Jeong SJ, Kwon GH, Lee HJ, Chang HC, Chung DK, Lee J-H, Kim JH. Characterization of paraplantaricin C7, a novel bacteriocin produced by Lactobacillus paraplantarum C7 isolated from kimchi. J. Microbiol. Biotechn. 17: 287-296 (2007)   과학기술학회마을
12 LoCascio RG, Mills DA, Waterhouse AL. Reduction of catechin, rutin, and quercetin levels by interaction with food-related microorganisms in a resting state. J. Sci. Food Agr. 86: 2105-2112 (2006)   DOI   ScienceOn
13 Kim M, Chun J. Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis. Int. J. Food Microbiol. 103: 91-96 (2005)   DOI   ScienceOn
14 Sasaki E, Shimada T, Osawa R, Nishitani Y, Spring S, Lang E. Isolation of tannin-degrading bacteria isolated from feces of the Japanese large wood mouse, Apodemus speciosus, feeding on tannin-rich acorns. Syst. Appl. Microbiol. 28: 358-365 (2005)   DOI   ScienceOn
15 Kar B, Banerjee R, Bhattacharyya BC. Optimization of physicochemical parameters for gallic acid production by evolutionary operation-factorial design technique. Process Biochem. 37: 1395-1401 (2002)   DOI
16 Short C. The probiotic century: Historical and current perspectives. Trends Food Sci. Tech. 10: 411-417 (1999)   DOI   ScienceOn
17 Um SH, Shin WS, Lee J-H. Real-time PCR monitoring of Lactobacillus sake, Lactobacillus plantarum, and Lactobacillus paraplantarum during kimchi fermentation. Food Sci. Biotechnol. 15: 595-598 (2006)   과학기술학회마을
18 Rodriguez H, de las Rivas B, Gomez-Cordoves C, Munoz R. Degradation of tannic acid by cell-free extracts of Lactobacillus plantarum. Food Chem. 107: 664-670 (2008)   DOI   ScienceOn
19 Mingshu L, Kai Y, Qiang H, Dongying J. Biodegradation of gallotannins and ellagitannins. J. Basic Microb. 46: 68-84 (2006)   DOI   ScienceOn
20 Bhat TK, Singh B, Sharma OP. Microbial degradation of tannins a current perspective. Biodegradation 9: 343-357 (1998)   DOI   ScienceOn
21 Goel G, Puniya AK, Aguilar CN, Singh K. Interaction of gut microflora with tannins in feeds. Naturwissenschaften 92: 497-503 (2005)   DOI   ScienceOn
22 Aguilar CN, Rodriguez R, Gutierrez-Sanchez G, Auguar C, Favela-Torres E, Prado-Barragan LA, Ramirez-Coronel A, Contreras-Esquivel JC. Microbial tannases: Advances and perspectives. Appl. Microbiol. Biot. 76: 47-59 (2007)   DOI   ScienceOn
23 Lee J-S, Heo G-Y, Lee JW, Oh Y-J, Park JA, Park Y-H, Pyun Y-R, Ahn JS. Analysis of kimchi microflora using denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 102: 143-150 (2005)   DOI   ScienceOn
24 Ephraim E, Odenyo A, Ashenafi M. Isolation and characterization of tannin-degrading bacteria from faecal samples of some wild ruminants in Ethiopia. Anim. Feed Sci. Tech. 118: 243-253 (2005)   DOI