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Evaluation of the Atopic Dermatitis-mitigating and Anti-inflammatory Effects of Kyung Hee Allergic Disease Herbal Formula (KAHF)  

Koh, Duck-Jae (Department of Pediatrics, Hospital of Oriental Medicine, Kyung Hee Medical Center)
Kim, Yang-Hee (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University)
Kim, Deog-Gon (Department of Pediatrics, Hospital of Oriental Medicine, Kyung Hee Medical Center)
Lee, Jin-Yong (Department of Pediatrics, Hospital of Oriental Medicine, Kyung Hee Medical Center)
Lee, Kyung-Tae (Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University)
Publication Information
Food Science and Biotechnology / v.17, no.6, 2008 , pp. 1272-1278 More about this Journal
Abstract
The purpose of this study was to investigate the effects of Kyung Hee Allergic Disease Herbal Formula (KAHF) on atopic dermatitis (AD) and its mode of action. Our clinical study showed KAHF reduced Severity Scoring of Atopic Dermatitis (SCORAD) indexes and subjective symptom scores. In parallel, the decreased levels of interferon (IFN)-$\gamma$ and interleukin (IL)-5 in serum, which contributed to its AD-mitigating effect was observed. To reveal the underlying mechanisms of KAHF in AD, its anti-inflammatory effect on lipopolysaccharide (LPS)-induced responses in RAW 264.7 cells was examined. KAHF was found to significantly inhibit the productions of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), and IL-$1{\beta}$ in LPS-stimulated RAW 264.7 macrophages. Consistently, KAHF potently inhibited protein and mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, KAHF inhibited LPS-induced activation of nuclear factor (NF)-$\kappa}B$. Taken together, our data suggest that KAHF has a beneficial effect on several eicosanoid-related skin inflammations, such as atopic dermatitis.
Keywords
atopic dermatitis; anti-inflammatory; prostaglandin $E_2$; factor-nuclear ${\kappa}B$; cytokine;
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1 Mosmann TR, Coffman RL. TH1 and TH2 cells: Different patterns of lymphokine secretion lead to different functional properties. Annu. Rev. Immunol. 7: 145-173 (1989)   DOI   ScienceOn
2 Sanderson CJ. Interleukin-5, eosinophils, and disease. Blood 79: 3101-3109 (1992)
3 Baeuerle PA, Baltimore D. $NF-{\kappa}B:$ Ten years after. Cell 87: 13-20 (1996)   DOI   ScienceOn
4 Nolan GP, Ghosh S, Liou HC, Tempst P, Baltimore D. DNA binding and I kappa B inhibition of the cloned p65 subunit of NF-kappaB, a rel-related polypeptide. Cell 64: 961-969 (1991)   DOI   ScienceOn
5 Beck LA, Leung DY. Allergen sensitization through the skin induces systemic allergic responses. J. Allergy Clin. Immun. 106: S258-S263 (2000)   DOI   ScienceOn
6 Grewe M, Gyufko K, Schopf E, Krutmann J. Lesional expression of interferon-gamma in atopic eczema. Lancet 343: 25-26 (1994)   DOI
7 Kimura M, Tsuruta S, Yoshida T. Unique profile of IL-4 and IFN-gamma production by peripheral blood mononuclear cells in infants with atopic dermatitis. J. Allergy Clin. Immun. 102: 238-244 (1998)   DOI   ScienceOn
8 Thepen T, Bihari IC, van Wichen DF, van Reijse FC. Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial Th2 response to a Th1 response in situ: An immunocytochemical study. J. Allergy Clin. Immun. 97: 828-837 (1996)   DOI   ScienceOn
9 Ohmen JD, Hanifin JM, Nickoloff BJ. Overexpression of IL-10 in atopic dermatitis. Contrasting cytokine patterns with delayed-type hypersensitivity reactions. Immunology 154: 1956-1963 (1995)
10 Bratton DL, May KR, Kailey JM, Doherty DE, Leung DY. Staphylococcal toxic shock syndrome toxin-1 inhibits monocyte apoptosis. J. Allergy Clin. Immun. 103: 895-900 (1999)   DOI   ScienceOn
11 Taniuchi S, Kojima T, Kobayashi Y. Increased serum nitrate levels in infants with atopic dermatitis. Allergy 56: 693-695 (2001)   DOI   ScienceOn
12 Wei XQ, Charles IG, Smith A, Ure J, Feng GJ, Huang FP, Xu D, Muller W, Moncada S, Liew FY. Altered immune responses in mice lacking inducible nitric oxide synthase. Nature 375: 408-411 (1995)   DOI   ScienceOn
13 Dinarello CA. Biologic basis for interleukin-1 in disease. Blood 87: 2095-2147 (1996)
14 Hunter CA, Chizzonite R, Remington JS. IL-1 beta is required for IL-12 to induce production of IFN-gamma by NK cells. A role for IL-1 beta in the T cell-independent mechanism of resistance against intracellular pathogens. Immunology 155: 4347-4354 (1995)
15 Barnes PJ, Karin M. Nuclear factor-kappaB: A pivotal transcription factor in chronic inflammatory diseases. New Engl. J. Med. 336: 1066-1071 (1997)   DOI   ScienceOn
16 Shin KM, Kim YH, Park WS, Kang I, Ha J, Choi JW, Park HJ, Lee KT. Inhibition of methanol extract from the fruits of Kochia scoparia on lipopolysaccharide-induced nitric oxide, prostaglandin $E_2$, and tumor necrosis $factor-{\alpha}$ production from murine macrophage RAW 264.7 Cells. Biol. Pharm. Bull. 27: 538-543 (2004)   DOI   ScienceOn
17 Zhang W, Leonard T, Bath-Hextall F, Chambers CA, Lee C, Humphreys R, Williams HC. Chinese herbal medicine for atopic eczema. Cochrane DB Syst. Rev. 4: CD002291 (2004)
18 Wardlaw AJ, Moqbel R, Kay AB. Eosinophils: Biology and role in disease. Immunology 60: 151-266 (1995)   DOI
19 Prehn A, Seger RA, Faber J, Torresani T, Molinari L, Gerber A, Sennhauser FH. The relationship of serum-eosinophil cationic protein and eosinophil count to disease activity in children with bronchial asthma. Pediatr. Allergy Immu. 4: 197-203 (1998)
20 Wong HC, Wong JK, Wong NY. Chinese herbal medicine for irritable bowel syndrome. J. Am. Med. Assoc. 282: 1036-1037 (1999)
21 O'Garra A. Cytokines induce the development of functionally heterogeneous T helper cell subsets. Immunity 8: 275-283 (1998)   DOI   ScienceOn
22 Smart JM, Kemp AS. Increased Th1 and Th2 allergen-induced cytokine responses in children with atopic disease. Clin. Exp. Allergy 32: 796-802 (2002)   DOI   ScienceOn
23 Roper RL, Phipps RP. Prostaglandin $E_2$ regulation of the immune response. Adv. Prostag. Thromb. L. 22: 101-111 (1994)
24 Friedlander SF. Corticosteroids: Options in the era of steroid-sparing therapy. J. Am. Acad. Dermatol. 53: S50-S58 (2005)   DOI   ScienceOn
25 Bratton DL, Hamid Q, Boguniewicz M, Doherty DE, Kailey JM, Leung DY. Granulocyte macrophage colony-stimulating factor contributes to enhanced monocyte survival in chronic atopic dermatitis. J. Clin. Invest. 95: 211-218 (1995)   DOI   ScienceOn
26 Staniforth V, Wang SY, Shyur LF, Yang NS. Shikonins, phytocompounds from Lithospermum erythrorhizon, inhibit the transcriptional activation of human tumor necrosis factor alpha promoter in vivo. J. Biol. Chem. 279: 5877-5885 (2004)   DOI   ScienceOn
27 Matsuda H, Dai Y, Ido Y, Ko S, Yoshikawa M, Kubo M. Studies on Kochiae fructus. IV. Anti-allergic effects of 70% ethanol extract and its component, momordin Ic from dried fruits of Kochia scoparia L. Biol. Pharm. Bull. 20: 1165-1170 (1997)   DOI   ScienceOn
28 Shekelle PG, Morton SC, Suttorp MJ, Buscemi N, Friesen C. Challenges in systematic reviews of complementary and alternative medicine topics. Ann. Intern. Med. 142: 1042-1047 (2005)   DOI   ScienceOn
29 Roper RL, Brown DM, Phipps RP. Prostaglandin $E_2$ promotes B lymphocyte Ig isotype switching to IgE. Immunology 154: 162-170 (1995)
30 Sucher NJ. Insights from molecular investigations of traditional Chinese herbal stroke medicines: Implications for neuroprotective epilepsy therapy. Epilepsy Behav. 8: 350-362 (2006)   DOI   ScienceOn
31 Hamid Q, Boguniewicz M, Leung DY. Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis. J. Clin. Invest. 94: 870-876 (1994)   DOI   ScienceOn
32 Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO. Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide. J. Invest. Dermatol. 126: 1792-1803 (2006)   DOI   ScienceOn
33 Cooper KD. Atopic dermatitis: Recent trends in pathogenesis and therapy. J. Invest. Dermatol. 102: 128-137 (1994)   DOI   ScienceOn
34 Grewe M, Schopf E, Thepen T, Ruzicka T. A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis. Immunol. Today 19: 359-361 (1998)   DOI   ScienceOn
35 Kimura M, Tsuruta S, Yoshida T. Correlation of house dust mite-specific lymphocyte proliferation with IL-5 production, eosinophilia, and the severity of symptoms in infants with atopic dermatitis. J. Allergy Clin. Immun. 101: 84-89 (1998)   DOI   ScienceOn
36 Hanifin JM, Rajka G. Diagnostic features of atopic dermatitis. Acta Derm. Venereol. 2: 44-47 (1980)
37 Novak N, Kraft S, Bieber T. Unraveling the mission of $Fc{\varepsilon}RI$ on antigen-presenting cells. J. Allergy Clin. Immun. 111: 38-44 (2003)   DOI   ScienceOn
38 Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, Akira S. A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-745 (2000)   DOI   ScienceOn
39 Leung DY, Boguniewicz M, Howell MD, Nomura I, Hamid QA. New insights into atopic dermatitis. J. Clin. Invest. 113: 651-657 (2004)   DOI
40 Stalder JF, Atherton T. Severity scoring of atopic dermatitis: The SCORAD index. Consensus report of the european task force on atopic dermatitis. Dermatology 186: 23-31 (1993)   DOI   ScienceOn
41 Chung HS, Kang M, Cho C, Park S, Kim H, Yoon YS, Kang J, Shin MK, Hong MC, Bae H. Inhibition of lipopolysaccharide and interferon-gamma-induced expression of inducible nitric oxide synthase and tumor necrosis factor-alpha by Lithospermi radix in mouse peritoneal macrophages. J. Ethnopharmacol. 102: 412-417 (2005)   DOI   ScienceOn
42 Kaptchuk TJ. Chinese herbal medicine for irritable bowel syndrome. J. Am. Med. Assoc. 282: 1035-1036 (1999)   DOI
43 Won JH, Im HT, Kim YH, Yun KJ, Park HJ, Choi JW, Lee KT. Anti-inflammatory effect of buddlejasaponin IV through the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via the NF-kappaB inactivation. Brit. J. Pharmacol. 148: 216-225 (2006)   DOI   ScienceOn
44 Matsuda H, Dai Y, Ido Y. Studies on Kochiae fructus. III. Antinociceptive and antiinflammatory effects of 70% ethanol extract and its component, momordin Ic from dried fruits of Kochia scoparia L. Biol. Pharm. Bull. 20: 1086-1091 (1997)   DOI   ScienceOn
45 Herz U, Bunikowski R, Renz H. Role of T cells in atopic dermatitis. Int. Arch. Allergy Imm. 115: 179-190 (1998)   DOI   ScienceOn
46 Spergel JM, Mizoguchi E, Oettgen H, Bhan AK, Geha RS. Roles of Thl and Th2 cytokines in a murine model of allergic dermatitis. J. Clin. Invest. 103: 1103-1111 (1999)   DOI   ScienceOn
47 Aral M, Arican O, Gul M, Sasmaz S, Kocturk SA, Kastal U, Ekerbicer HC. The relationship between serum levels of total IgE, IL-18, IL-12, IFN-gamma, and disease severity in children with atopic dermatitis. Mediat. Inflamm. 4: 73098 (2006)