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Anti-tumor and Anti-inflammatory Activity of the Methanol Extracts from Adlay Bran  

Lee, Ming-Yi (Graduate Institute of Food Science and Technology, Center for Food and Biomolecules, College of Bioresources and Agriculture, National Taiwan University)
Tsai, Shu-Hsien (Institute of Microbiology and Biochemistry, College of Life Science, National Taiwan University)
Kuo, Yueh-Hsiung (Department of Chemistry, National Taiwan University)
Chiang, Wenchang (Graduate Institute of Food Science and Technology, Center for Food and Biomolecules, College of Bioresources and Agriculture, National Taiwan University)
Publication Information
Food Science and Biotechnology / v.17, no.6, 2008 , pp. 1265-1271 More about this Journal
Abstract
Adlay bran is a waste product previously thought to have no commercial value, Its methanolic extract was fractionated using n-hexane (ABM-Hex), ethyl acetate (ABM-EtOAc), 1-butanol (ABM-BuOH), and water (ABM-$H_2O$). The ABM-EtOAc fraction exhibited a strongest inhibition against growth of human lung cancer cell A549 and human colorectal carcinoma cells HT-29 and COLO 205. Inhibition of cell cycle progression at $G_0/G_1$ transition, increase of cells at the sub-$G_1$ phase, and DNA ladders were observed in cells treated with ABM-EtOAc. The ABM-BuOH fraction showed the strongest inhibition of proinflammatory cytokines tumor necrosis factor (TNF)-$\alpha$ and interlukin (IL)-$1{\beta}$ in stimulated RAW 264.7 macrophages. Further, ABM-EtOAc and ABM-BuOH inhibited cyclooxygenase (COX)-2 expression in A549 and HT-29 carcinoma cells, while COX-l expression was not affected. These results reveal that both ABM-EtOAc and ABM-BuOH may aid the prevention of cancers and the applications in cancer chemotherapy.
Keywords
A549 cell; COLO 205 cell; cyclooxygenase-2 (COX-2); cytotoxicity; HT-29 cell;
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