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Isolation of Alcohol-tolerant Amylolytic Saccharomyces cerevisiae and Its Application to Alcohol Fermentation  

Jung, He-Kyoung (Bio Industry Center, Daegu Technopark)
Park, Chi-Duck (Bio Industry Center, Daegu Technopark)
Bae, Dong-Ho (Department of Bioscience and Biotechnology, Konkuk University)
Hong, Joo-Heon (Bio Industry Center, Daegu Technopark)
Publication Information
Food Science and Biotechnology / v.17, no.6, 2008 , pp. 1160-1164 More about this Journal
Abstract
An novel amylolytic yeast, Saccharomyces cerevisiae HA 27, isolated from nuruk, displayed resistance against high sugar (50% glucose) and alcohol (15%). Maximal production of amylolytic enzyme by S. cerevisiae HA 27 was achieved on 9 days of cultivation at the optimal temperature $20^{\circ}C$ and pH 6.0. The activity of amylolytic enzyme produced by S. cerevisiae HA 27 was stable, even at $70^{\circ}C$, and over a broad pH range (4.0-11.0). Also, the amylolytic enzyme of S. cerevisiae HA 27 showed optimal activity in pH 5.0 at $50^{\circ}C$. S. cerevisiae HA 27 exhibited 6.2%(v/v) alcohol fermentation ability using starch as a carbon source.
Keywords
amylolytic yeast; nuruk; Saccharomyces cerevisiae; alcohol-tolerance;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
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1 Kim T-G, Kim K. The construction of starch-fermenting yeast using genetic engineering and rare-mating. Appl. Biochem. Biotech. 59: 39-51 (1996)   DOI   ScienceOn
2 Ronald M. Microbiological Media. CRC Press, NW, USA. p. 1624 (1993)
3 Seo D-H, Jung J-H, Kim H-Y, Kim Y-R, Ha S-J, Kim Y-C, Park C-S. Identification of lactic acid bacteria involved in traditional Korean rice wine fermentation. Food Sci. Biotechnol. 16: 994-998 (2007)   과학기술학회마을
4 Choi S-H, Sung C, Oh M-J, Kim C-J. Intergenic protoplast fusion in Saccharomycopsis fibuligera and Saccharomyces cerevisiae. J. Ferment. Bioeng. 84: 158-161 (1997)   DOI   ScienceOn
5 Seo J-H, Kim Y-H, Hong S-D, Kwon T-K. A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast. (Part4) Alcohol and pullulanase productivities of fusant between S. diastaticus and C. tropicalis. Korean J. Microbiol. Biotechnol. 14: 365-369 (1986)
6 Kim Y-H, Seu J-H. Culture conditions for glucoamylase production and ethanol productivity of heterologous transformant of Saccharomyces cerevisiae by glucoamylase gene of Saccharomyces diastaticus. Korean J. Microbiol. Biotechnol. 16: 494-498 (1988)
7 Koo Y-J, Park W-S, Shin D-H, Yu T-J. Isolation and identification of the amylolytic yeast Hansenula and its Haploid mutant. Koreran J. Microbiol. Biotechnol. 13: 129-135 (1985)
8 Park S-Y, Choi S-Y, Min J-H. Isolation of glucoamyalse producing yeasts and its enzymatic characteristics. Korean J. Mycol. 27: 386-393 (1999)
9 Park S-Y, Kim M-S, Kim K. Direct ethanol production from starch substrate by polyploid recombinant yeast secreting both ${\alpha}-amylase$ and glucoamylase. Korean J. Appl. Microbiol. Biotechnol. 24: 604-612 (1996)
10 Seu J-H, Kim Y-H, Jun D-Y, Lee J-T. A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast. (Part1) Isolation and characterization of fusant between S. cecevisiae and S. diastaticus. Korean J. Microbiol. Biotechnol. 14: 305-310 (1986)
11 Park W-S, Koo Y-J, Shin D-H, Min B-Y. Study on the cultural conditions of starch utilizing yeast Sporobolomyces holsaticus. Korean J. Food Sci. Technol. 15: 51-55 (1993)
12 Abarca D, Lobato MF, Claros MG, Jimenez A. Isolation and expression in Saccharomyces cerevisiae of a gene encoding an ${\alpha}-amylase$ from Schwaniomyces castellii. FEBS Lett. 255: 455-459 (2005)
13 Asgher M, Asad MJ, Rahman SU, Legge RL. A thermostable ${\alpha}-amylase$ from a moderately thermophilic Bacillus subtilis strain for starch processing. J. Food Eng. 79: 950-955 (2007)   DOI   ScienceOn
14 Park W-S, Koo Y-J, Shin D-H, Suh K-B. Isolation and identification of starch utilizing yeast. Korean J. Food Sci. Technol. 15: 46-50 (1983)
15 NTSI. Analysis, Assessment, and Research of Alcoholic Beverages and Brewing Raw Materials. National Tax Service Institute, Seoul, Korea. pp. 12-63 (1979)
16 Yu H-E, Lee D-H, Lee J-H, Choi S-Y, Lee J-S. Quality characteristics and cardiovascular activities of Korean traditional wines and liquors. Food Sci. Biotechnol. 14: 772-777 (2005)
17 Murai T, Yoshino T, Ueda M, Haranoya I, Ashikari T, Yoshizumi H, Tanaka A. Evaluation of the function of arming yeast displaying glucoamylase on its cell surface by direct fermentation of corn to ethanol. J. Ferment. Bioeng. 86: 569-572 (1998)   DOI   ScienceOn
18 Seu J-H, Kwon T-K, Hong S-D. A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast. (Part3) Isolation and characterization of fusant between S. diastaticus and C. tropicalis. Korean J. Microbiol. Biotechnol. 14: 359-363 (1986)
19 Kim K, Lee J-H. Construction of a transformed yeast strains secreating both ${\alpha}-amylase$ and glucoamylase for direct starch-fermentation. J. Microbiol. Biotechn. 4: 7-12 (1994)
20 Ha D-M, Kim D-C, Hong S-M, Lee C-W. Identification and properties of starch utilizing yeasts isolated from nuruk. J. Korean Soc. Appl. Biol. Chem. 32: 408-415 (1989)
21 Han Y-J, Yu T-S. Characterization of two forms of glucoamylase from traditional Korean nuruk fungi, Asepergillus corenus NR 15-1. J. Microbiol. Biotechn. 15: 239-246 (2005)   과학기술학회마을
22 Park W-S, Koo Y-J, Shin D-H, Min B-Y. Study on the pattern of starch assimilation by Sporobolomyces holsaticus. Korean J. Food Sci. Technol. 15: 177-182 (1983)
23 Peres MFS, Souza CS, Thomaz D, Souza AR, Cecilia L. Partitioning of the glucoamylase activity at the cell surfaces in cultures of Saccharomyces. Process Biochem. 41: 20-27 (2006)   DOI