Browse > Article

Biological Activities and Partial Characterization of Beauveria bassiana Mycelium  

Park, Sung-Yong (Ottogi Research Center)
Song, Hyuk-Hwan (Department of Food Science and Technology, Chung-Ang University)
Lee, Yong-Gab (Ottogi Research Center)
Yoon, Cheol-Sik (Research Institute of Engineering and Technology, Korea University)
Lee, Chan (Department of Food Science and Technology, Chung-Ang University)
Publication Information
Food Science and Biotechnology / v.17, no.1, 2008 , pp. 95-101 More about this Journal
Abstract
Some biological activities of Beauveria bassiana were studied to elucidate pharmacological function of B. bassiana-infected larva of the silkworm. The mycelium consisted mainly of carbohydrate (65.8%), followed by protein (15.9%) and fat (8.3%). Glucose (68.8%), mannose (7.1%), and galactose (6.1%) were major components in carbohydrates. Ten amino acids including glutamine, threonine, valine, aspartic acid, alanine, leucine, serine, glycine, arginine, and isoleucine were found in protein as major amino acids. Various extracts were prepared from the freeze-dried mycelium of B. bassiana by systemic extraction and their biological activities were investigated. Among tested fractions, the hot-water extract (HW) contributed significantly to the anti-coagulant activity, anti-complementary activity, and stimulation of intestinal immune system. The methanol extract (ME) increased acetylcholinesterase (AChE) inhibition activity and reactive oxygen species (ROS) scavenging activity.
Keywords
Beauveria bassiana; anti-coagulant; anti-complement; instestinal immunity; acetylcholinesterase;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Yamaguchi Y, Kagota S, Nakamura K, Shinozuka K, Kunitomo M. Antioxidant activity of the extracts from fruiting bodies of cultured Cordyceps sinensis. Phytother. Res. 14: 647-649 (2000)   DOI   ScienceOn
2 Zhang Q, Wu J, Hu Z, Li D. Induction of HL-60 apoptosis by ethyl acetate extract of Cordyceps sinensis fungal mycelium. Life Sci. 75: 2911-2919 (2004)   DOI   ScienceOn
3 Ohmori T, Tamura K, Tsuru S, Nomoto K. Antitumor activity of protein-bound polysaccharide from Cordyceps ophioglossoides in mice. Jpn. J. Cancer Res. 77: 1256-1263 (1986)
4 AOAC. Official Methods of Analysis of AOAC Intl. 16th ed. Association of Official Analytical Communities, Arlington, VA, USA (1995)
5 Kiyohara H, Yamada H, Cyong JC, Otsuka Y. Studies on polysaccharides from Angelica acutiloba. V. Molecular aggregation and anti-complementary activity of arabinogalactan from Angelica acutiloba. J. Pharmacobio-Dynam. 9: 339-345 (1986)   DOI
6 Hong T, Matsumoto T, Kiyohara H, Yamada H. Enhanced production of hematopoietic growth factors through T cell activation in Peyer's patches by oral administration of kampo (Japanese herbal) medicine, 'Juzen-Taiho-To'. Phytomedicine 5: 353-360 (1998)   DOI   ScienceOn
7 Jun W-J, Kim S-H, Lee D-H, Chung J-W, Sim S-I, Lee K-W, Cho H-Y, Hong B-S. In vitro and in vivo effects of Gelidium amansii on intestinal immune system. Food Sci. Biotechnol. 14: 147-151 (2005)
8 Gonda R, Tomoda M, Shimizu N, Yamada H. Structure and anticomplementary activity of an acidic polysaccharide from the leaves of Malva sylvestris var. mauritiana. Carbohyd. Res. 198: 323-329 (1990)   DOI   ScienceOn
9 Yamada H, Ra KS, Kiyohara H, Cyong JC, Otsuka Y. Structural characterization of an anti-complementary pectic polysaccharide from the roots of Bupleurum falcatum L. Carbohyd. Res. 189: 209- 226 (1989)   DOI   ScienceOn
10 Koh J-H, Yu K-W, Suh H-J, Choi Y-M, Ahn T-S. Activation of macrophages and the intestinal immune system by an orally administered decoction from cultured mycelia of Cordyceps sinensis. Biosci. Biotech. Bioch. 66: 407-411 (2002)   DOI   ScienceOn
11 Coyle JT, Price DL, DeLong MR. Alzheimer's disease: A disorder of cortical cholinergic innervation. Science 219: 1184-1190 (1983)   DOI
12 Ohigashi H, Osawa T, Terao J, Watanabe S, Yoshikawa T. Food Factors for Cancer Prevention. Springer, Tokyo, Japan. pp. 188-193 (1997)
13 Shigenaga MK, Hagen TM, Ames BN. Oxidative damage and mitochondrial decay in aging. P. Natl. Acad. Sci. USA 91: 10771- 10778 (1994)   DOI   ScienceOn
14 Fernandez S, Groden E, Vandenberg JD, Furlong MJ. The effect of mode of exposure to Beauveria bassiana on conidia acquisition and host mortality of Colorado potato beetle, Leptinotarsa decemlineata. J. Invertebr. Pathol. 77: 217-226 (2001)   DOI   ScienceOn
15 Koh J-H, Kim J-M, Chang U-J, Suh H-J. Hypocholesterolemic effect of hot-water extract from mycelia of Cordyceps sinensis. Biol. Pharm. Bull. 26: 84-87 (2003)   DOI   ScienceOn
16 Choi H-S, Shin H-H. Purification and characterization of a fibrinolytic protease in Pleurotus ostreatus. Mycologia 90: 647-679 (1998)
17 Kweon M-H, Hwang H-J, Sung H-C. Isolation and characterization of anticomplementary beta-glucans from the shoots of bamboo Phyllostachys edulis. Planta Med. 69: 56-62 (2003)   DOI   ScienceOn
18 Yu K-W, Kim K-M, Suh H-J. Pharmacological activities of stromata of Cordyceps scarabaecola. Phytother. Res. 17: 244-249 (2003)   DOI   ScienceOn
19 Ellman GL, Courtney KD, Andres V Jr, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7: 88-95 (1961)   DOI   ScienceOn
20 Pearay O, Jiri M, Michael L, Warren S, Jerry M, John B. Handbook of Mucosal Immunology. Academic Press, London, UK. pp. 275- 285 (1994)
21 Choi H-S, Sa Y-S. Fibrinolytic and antithrombotic protease from Ganoderma lucidum. Mycologia 92: 545-552 (2000)   DOI
22 Eldeen IMS, Elgorashi EE, van Staden J. Antibacterial, antiinflammatory, anti-cholinesterase, and mutagenic effects of extracts obtained from some trees used in South African traditional medicine. J. Ethnopharmacol. 102: 457-464 (2005)   DOI   ScienceOn
23 Yamada H, Kiyohara H, Cyong JC, Kojima Y, Kumazawa Y, Otsuka Y. Studies on polysaccharides from Angelica acutiloba. Part I. Fractionation and biological properties of polysaccharides. Planta Med. 50: 163-168 (1984)   DOI   ScienceOn
24 Zhao-Long W, Xiao-Xia W, Wei-Ying C. Inhibitory effect of Cordyceps sinensis and Cordyceps militaris on human glomerular mesangial cell proliferation induced by native LDL. Cell Biochem. Funct. 18: 93-97 (2000)   DOI   ScienceOn
25 Ha Y-W, Jeon B-T, Moon S-H, Toyoda H, Toida T, Linhardt RJ, Kim Y-S. Characterization of heparin sulfate from the unossified antler of Cervus elaphus. Carbohyd. Res. 340: 411-416 (2005)   DOI   ScienceOn
26 Tarr GE, Shively JE. Methods of Protein Microcharacterization. Humana Press, Clifton, NJ, USA. pp. 155-194 (1986)
27 Pemberton RW. Insects and other arthropods used as drugs in Korean traditional medicine. J. Ethnopharmacol. 65: 207-216 (1999)   DOI   ScienceOn
28 Lee H-S. Anticoagulant properties of compounds derived from Fennel (Foeniculum vulgare Gaertner) fruits. Food Sci. Biotechnol. 15: 763-767 (2006)   과학기술학회마을
29 Chemnitius JM, Haselmeyer KH, Gonska BD, Kreuzer H, Zech R. Indirect parasympathomimetic activity of metoclopramide: Reversible inhibition of cholinesterase from human central nervous system and blood. Pharmacol. Res. 34: 65-72 (1996)   DOI   ScienceOn
30 Yoo H-S, Shin J-W, Cho J-H, Son C-G, Lee Y-W, Park S-Y, Cho CK. Effects of Cordyceps militaris extract on angiogenesis and tumor growth. Acta Pharmacol. Sin. 25: 657-665 (2004)
31 Marker M, Andrews PC, Babior BM. Measurement of $O_2$ production by human neutrophils. The preparation and assay of NADPH oxidase-containing particles from human neutrophils. Method Enzymol. 105: 358-365 (1984)   DOI
32 Howes MR, Houghton PJ. Plants used in Chinese and Indian traditional medicine for improvement of memory and cognitive function. Pharmacol. Biochem. Be. 75: 513-527 (2003)   DOI   ScienceOn
33 Won S-Y, Park E-H. Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris. J. Ethnopharmacol. 96: 555-561 (2005)   DOI   ScienceOn
34 Chun H, Shin D-H, Hong B-S, Cho H-Y, Yang H-C. Purification and biological activity of acidic polysaccharide from leaves of Thymus vulgaris L. Biol. Pharm. Bull. 24: 941-946 (2001)   DOI   ScienceOn
35 Yoon C-S, Yu K-W, Bae S-H, Song H-H, Park H-S, Lee C. Chemical properties and physiological activities of synnemata of Beauveria bassiana. J. Microbiol. Biotechn. 13: 125-133 (2003)
36 Fox I, Dawson A, Loynds R, Eisneer J, Findlen K, Levin E, Hanson D, Mant T, Wagner J, Maragaonre J. Anticoagulant activity of HirulogTM, a direct thrombin inhibitor, in human. Thromb. Haemostasis 69: 157-165 (1993)
37 Hirsh J, Fuster Vl. Guide to anticoagulant therapy. Part I: Heparin. Circulation 89: 1449-1468 (1994)   DOI   ScienceOn
38 Rubio LA. Determination of diaminopimelic acid in rat feces by high performance liquid chromatography using the Pico-Tag method. J. Chromatogr. B 784: 125-129 (2003)
39 Sung J-M, Kim C-H, Yang K-J, Lee H-K, Kim Y-S. Studies on distribution and utilization of Cordyceps militaris and Cordyceps mutans. Korean Mycol. 21: 94-105 (1993)   과학기술학회마을
40 Lim S-S, Han S-M, Kim S-Y, Bae Y-S, Kang I-J. Isolation of acetylcholinesterase inhibitors from the flowers of Chrysanthemum indicum Linne. Food Sci. Biotechnol. 16: 256-269 (2007)   과학기술학회마을
41 Halliwell B. Reactive oxygen species and the central nervous system. J. Neurochem. 59: 1609-1623 (1992)   DOI   ScienceOn