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Induction of Caspase-3 Dependent Apoptosis in Human Ovarian Cancer SK-OV-3 Cells by Genistein  

Choi, Eun-Jeong (Plant Resources Research Institute, Duksung Women's University)
Kim, Tae-Hee (Plant Resources Research Institute, Duksung Women's University)
Kim, Gun-Hee (Plant Resources Research Institute, Duksung Women's University)
Chee, Kew-Mahn (Department of Biotechnology and Life Sciences, Korea University)
Publication Information
Food Science and Biotechnology / v.17, no.1, 2008 , pp. 216-218 More about this Journal
Abstract
The present study was designed to determine how the phytochemical genistein activates caspase-3 to cause cell cycle arrest and apoptosis. When human ovarian cancer SK-OV-3 cells were treated with $200\;{\mu}M$ genistein for 24 hr, cell growth decreased significantly (p<0.05). Conversely, genistein treatment significantly increased cytotoxicity (measured as lactate dehydrogenase release) under the same conditions (p<0.05). To elucidate the mechanism behind the induction of apoptosis by genistein, we studied the cell cycle and caspase-3 activation. When cells were treated with genistein, the population of cells in sub-G1 phase increased by 44.2% compared to untreated cells. Genistein caused decrease in precursor caspase-3, increase in cleaved caspase-3 and a significant increase in caspase-3 activity (p<0.05). Therefore, genistein may induce apoptosis via caspase-3 activation. However, high-dose genistein treatment must be viewed with caution because of its potential cytotoxicity.
Keywords
apoptosis; caspase-3; cytotoxicity; genistein; SK-OV-3 cell;
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Times Cited By Web Of Science : 0  (Related Records In Web of Science)
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