Browse > Article

Neuroprotective Effect of Scopoletin from Angelica dahurica on Oxygen and Glucose Deprivation-exposed Rat Organotypic Hippocampal Slice Culture  

Son, Dong-Wook (KT&G Central Research Institute)
Lee, Pyeong-Jae (Department of Natural Medicine Resources, Semyung University)
Lee, Jong-Seok (Department of Anesthesiology and Critical Care Medicines, School of Medicine, Johns Hopkins University)
Lee, Sang-Hyun (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University)
Choi, Sang-Yoon (Korea Food Research Institute)
Lee, Jong-Won (KT&G Central Research Institute)
Kim, Sun-Yeou (Department of Herbal Pharmacology, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Food Science and Biotechnology / v.16, no.4, 2007 , pp. 632-635 More about this Journal
Abstract
This study examined the neuroprotective effect of scopoletin from Angelica dahurica against oxygen and glucose deprivation-induced neurotoxicity in a rat organotypic hippocampal slice culture. Scopoletin reduced the propidium iodide (PI) uptake, which is an indication of impaired cell membrane integrity. In addition, it inhibited the loss of NeuN, which represents the viability of neuronal cells. The results suggests that scopoletin from A. dahurica protects neuronal cells from the damage caused by oxygen and glucose deprivation.
Keywords
Angelica dahurica; scopoletin; oxygen-glucose deprivation; hippocampus slice culture; neuroprotection;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Kang TH, Pae HO, Jeong SJ, Yoo JC, Choi BM, Jun CD, Chung HT, Miyamoto T, Higuchi R, Kim YC. Scopoletin: an inducible nitric oxide synthesis inhibitory active constituent from Artemisia feddei. Planta Med. 65: 400-403 (1999)   DOI   ScienceOn
2 Noraberg J, Kristensen BW, Zimmer J. Markers for neuronal degeneration in organotypic slice cultures. Brain Res. Protoc. 3: 278-299 (1999)   DOI   ScienceOn
3 Pringle AK, Iannotti F, Sundstrom LE. Neuroprotection by both NMDA and non-NMDA receptor antagonists in in vitro ischemia. Brain Res. 755: 36-46 (1997)   DOI   ScienceOn
4 Nishizawa Y. Glutamate release and neuronal damage in ischemia. Life Sci. 69: 369-381 (2001)   DOI   ScienceOn
5 Bickler PE, Hansen BM. Causes of calcium accumulation in rat cortical brain slices during hypoxia and ischemia: role of ion channels and membrane damage. Brain Res. 665: 269-276 (1994)   DOI   ScienceOn
6 Rollinger JM, Hornick A, Langer T, Stuppner H, Prast H. Acetylcholinesterase inhibitory activity of scopolin and scopoletin discovered by virtual screening of natural products. J. Med. Chem. 47: 6248-6254 (2004)   DOI   ScienceOn
7 Arai K, Nishiyama N, Matsuki N, Ikegaya Y. Neuroprotective effects of lipoxygenase inhibitors against ischemic injury in rat hippocampal slice cultures. Brian Res. 904: 167-172 (2001)   DOI   ScienceOn
8 Gilgun-Sherki Y, Melamed E, Offen D. Oxidative stress inducedneurodegenerative diseases: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacology 40: 959-975 (2001)   DOI   ScienceOn
9 Park KJ, Ha H-C, Kim H-S, Chiba K, Yeo I-K, Lee S-Y. The neuroprotective and neurotrophic effects of Korean gardenia (Gardenia jasminoides Ellis) in PC12h cells. Food Sci. Biotechnol. 15: 735-738 (2006)   과학기술학회마을
10 Muschietti L, Gorzalczany S, Ferraro G, Acevedo C, Martino V. Phenolic compounds with anti-inflammatory activity from Eupatorium buniifolium. Planta Med. 67: 743-744 (2001)   DOI   ScienceOn
11 Vitale M, Zamai L, Mazzotti G, Cataldi A, Falcieri E. Differential kinetics of propidium iodide uptake in apoptotic and necrotic thymocytes. Histochemistry 100: 223-229 (1993)   DOI
12 Haddad GG, Jiang C. $O_2$ Deprivation in the central nervous system: on mechanisms of neuronal response, differential sensitivity, and injury. Prog. Neurobiol. 40: 277-318 (1993)   DOI   ScienceOn
13 Facchinetti F, Dawson VL, Dawson TM. Free radicals as mediators of neuronal injury. Cell. Mol. Neurobiol. 18: 667-682 (1998)   DOI   ScienceOn
14 Yrjanheikki J, Tikka T, Keinanen R, Goldsteins G, Chan PH, Koistinaho J. A tetracycline derivative, minocycline, reduces inflammation, and protects against focal cerebral ischemia with a wide therapeutic window. P. Natl. Acad. Sci. USA 96: 13496-13500 (1999)   DOI
15 Frotscher M, Zafirow S, Heimrich B. Development of identified neuronal types and of specific synaptic connections in slice cultures of rat hippocampus. Prog. Neurobiol. 45: 7-28 (1995)
16 Kwon YS, Shin SJ, Kim MJ, Kim CM. A new coumarin from the stem of Angelica dahurica. Arch. Pharm. Res. 25: 53-56 (2002)   DOI   ScienceOn
17 Kang SY, Sung SH, Park JH, Kim YC. Hepatoprotective activity of scopoletin, a constituent of Solanum lyratum. Arch. Pharm. Res. 21: 718-722 (1998)   DOI   ScienceOn
18 Kim NY, Pae HO, Ko YS, Yoo JC, Choi BM, Jun CD, Chung HT, Inagaki M, Higuchi R, Kim YC. In vitro inducible nitric oxide synthesis inhibitory active constituents from Fraxinus rhynchophylla. Planta Med. 65: 656-658 (1999)   DOI   ScienceOn
19 Iadecola C. Bright and dark sides of nitric oxide in ischemic brain injury. Trends Neurosci. 20: 132-139 (1997)   DOI   ScienceOn
20 Gahwiler BH, Capogna M, Debanne D, Mckinney RA, Thompson SM. Organotypic slice cultures: a technique has come of age. Trends Neurosci. 20: 471-477 (1997)   DOI   ScienceOn
21 Toda S. Inhibitory effects of phenylpropanoid metabolites on copper-induced protein oxidative modification of mice brain homogenate in vitro. Biol. Trace Elem. Res. 85: 183-188 (2002)   DOI   ScienceOn