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Effect of Fucoidan on Expression of Diabetes Mellitus Related Genes in Mouse Adipocytes  

Kim, Kui-Jin (Graduate School of Life Science and Biotechnology, Pochon CHA University)
Lee, Ok-Hwan (Graduate School of Life Science and Biotechnology, Pochon CHA University)
Lee, Han-Chul (Graduate School of Life Science and Biotechnology, Pochon CHA University)
Kim, Young-Cheul (Department of Nutrition, University of Massachusetts)
Lee, Boo-Yong (Graduate School of Complementary Alternative Medicine, College of Medicine, Pochon CHA University)
Publication Information
Food Science and Biotechnology / v.16, no.2, 2007 , pp. 212-217 More about this Journal
Abstract
Fucoidan (fucan sulfate) is a fucose-containing sulfated polysaccharide from brown algae such as Fucus vesiculosus, Ecklonia kurome, and Cladosiphon okamuranus. The aim of this study was to investigate the effect of fucoidan on the expression of diabetes-related genes in mouse cell line 3T3-L1. 3T3-L1 adipocytes were cultured for 48 hr with or without fucoidan (10, 100, and 500 ppm) on a 60 mm dish. Reverse transcription polymerase chain reaction (RT-PCR) was used for measurement of peroxisome proliferators activated receptor ${\gamma}\;(PPAR{\gamma})$, CCAAT/enhancer binding protein ${\alpha}\;(C/EBP{\gamma})$, and glucose transporter 4 (GLUT4) RT-PCR analysis revealed that expression level of GLUT4, $PPAR{\gamma}$, and $C/EBP{\alpha}$ mRNAs increased with fucoidan treatment from 10 to 500 ppm in a dose-dependent manner. Fucoidan appears to enhance insulin sensitivity by increasing the expression level of diabetes-related genes in 3T3-L1 adipocytes. Therefore, fucoidan is potentially useful as a natural therapeutic material for hyperglycemia in type II diabetes patients.
Keywords
fucoidan; 3T3-L1 cells; gene expression; $PPAR{\gamma}$; $C/EBP{\alpha}$; GLUT4;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
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1 Korean Diabetes Association, http://www.diabetes.or.kr/publication/index.html. Accessed Apr. 30, 2006
2 Jacobson PB, von Geldem TW, Ohman L, Osterland M, Wang J, Zinker B, Wilcox D, Nguyen PT, Mika A, Fung S, Fey T, GoosNilsson A, Grynfarb M, Barkhem T, Marsh K, Beno DW, NgaNguyen B, Kym PR, Link JT, Tu N, Edgerton DS, Cherrington A, Efendic S, Lane BC, Opgenorth TJ. Hepatic glucocorticoid receptor antagonism is sufficient to reduce elevated hepatic glucose output and improve glucose control in animal models of type 2 diabetes. J. Pharmacol. Exp. Ther. 314: 191-200 (2005)   DOI   ScienceOn
3 O'Moore-Sullivan TM, Prins JB. Thiazolidinediones and type 2 diabetes: new drugs for an old disease. Med. J. Australia 176: 381-386 (2002)
4 Kaestner KH, Christy RJ, Lane MD. Mouse insulin-responsive glucose transporter gene: characterization of the gene and transactivation by the CCAAT/enhancer binding protein. P. Natl, Acad. Sci. USA 87: 251-255 (1990)   DOI
5 Lemberger T, Braissant O, Juge AC, Keller H, Saladin R, Staels B, Auwerx J, Burger AG, Meier CA, Wahli W. PPAR tissue distribution and interactions with other hormone-signaling pathways. Ann. NY Acad. Sci. 804: 231-251 (1996)   DOI
6 Staels B, Schoonjans K, Fruchart JC, Auwerx J. The effects of fibrates and thiazolidinediones on plasma triglyceride metabolism are mediated by distinct peroxisome proliferator activated receptors (PPARs). Biochimie 79: 95-99 (1997)   DOI   ScienceOn
7 Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor. Cell 79: 1147-1156 (1994)   DOI   ScienceOn
8 Forman BM, Tontonoz P, Chen J, Brun RP, Spiegelman BM, Evans RM. 15-Deoxy-delta 12, 14-prostaglandin J2 is a ligand for the adipocyte determination factor PPAR gamma. Cell 83: 803-812 (1995)   DOI   ScienceOn
9 Abel ED, Peroni OD, Kim JK, Kim YB, Boss O, Hadro E, Minnemann T, Shulman GI, Kahn BB. Adipose-selective targeting of the GLUT4 gene impairs insulin action in muscle and liver. Nature 409: 729-733 (2001)   DOI   ScienceOn
10 Cao Z, Umek RM, McKnight SL. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Gene Dev. 5: 1538-1552 (1991)   DOI   ScienceOn
11 Ciaraldi T, Henry RR. Thiazolidinediones and their effects on glucose transporters. Eur. J. Endocrinol. 137: 610-612 (1997)   DOI
12 Konno S, Tortorelis DG, Fullerton SA, Samadi AA, Hettiarachchi J, Tazaki H. A possible hypoglycaemic effect of maitake mushroom on Type 2 diabetic patients. Diabetic. Med. 18: 1010 (2001)   DOI
13 Berger J, Biswas C, Vicario PP, Strout HV, Saperstein R, Pilch PF. Decreased expression of the insulin-responsive glucose transporter in diabetes and fasting. Nature 340: 70-72 (1989)   DOI   ScienceOn
14 Tontonoz P, Hu E, Graves RA, Budavari AI, Spiegelman BM. mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Gene Dev. 8: 1224-1234 (1994)   DOI
15 Hu E, Tontonoz P, Spiegelman BM. Transdifferentiation of myoblasts by the adipogenic transcription factors PPAR gamma and C/EBP alpha. P. Natl. Acad. Sci. USA 92: 9856-9860 (1995)   DOI
16 Watson RT, Kanzaki M, Pessin JE. Regulated membrane trafficking of the insulin-responsive glucose transporter 4 in adipocytes. Endocr. Rev. 25: 177-204 (2004)   DOI   ScienceOn
17 Patankar MS, Oehninger S, Bamett T, Williams RL, Clark GF. A revised structure for fucoidan may explain some of its biological activities. J. BioI. Chem. 268: 21770-21776 (1993)
18 Tamori Y, Masugi J, Nishino N, Kasuga M. Role of peroxisome proliferator-activated receptor-gamma in maintenance of the characteristics of mature 3T3-L1 adipocytes. Diabetes 51: 2045-2055 (2002)   DOI   ScienceOn
19 Haroun-Bouhedja F, Ellouali M, Sinquin C, Boisson-Vidal C. Relationship between sulfate groups and biological activities of fucans. Thromb. Res. 100: 453-459 (2000)   DOI   ScienceOn
20 Chawla A, Schwarz EJ, Dimaculangan DD, Lazar MA. Peroxisome proliferator-activated receptor (PPAR) gamma: adipose-predominant expression and induction early in adipocyte differentiation. Endocrinology 135: 798-800 (1994)   DOI   ScienceOn
21 Chao L, Marcus-Samuels B, Mason MM, Moitra J, Vinson C, Arioglu E, Gavrilova O, Reitman ML. Adipose tissue is required for the antidiabetic, but not for the hypolipidemic, effect of thiazolidinediones. J. Clin. Invest. 106: 1221-1228 (2000)   DOI   ScienceOn
22 Bojakowski K, Abramczyk P, Bojakowska M, Zwolinska A, Przybylski, J, Gaciong Z. Fucoidan improves the renal blood flow in the early stage of renal ischemia/reperfusion injury in the rat. J. Physiol. Phamarcol. 52: 137-143 (2001)
23 MacDougald OA, Lane MD. Transcriptional regulation of gene expression during adipocyte differentiation. Annu. Rev. Biochem. 64: 345-373 (1995)   DOI   ScienceOn
24 Kliewer SA, Lenfard JM, Willson TM, Patel I, Morris DC, Lehmann JM. A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor gamma and promotes adipocyte differentiation. Cell 83: 813-819 (1995)   DOI   ScienceOn
25 Wu Z, Nycher NLR, Fanner SR. Induction of peroxisome proliferatoractivated receptor gamma during the conversion of 3T3 fibroblasts into adipocytes is mediated by C/EBPbeta, C/EBPdelta, and glucocorticoids. Mol. Cell BioI. 16: 4128-4136 (1996)   DOI
26 Christy RJ, Kaestner KH, Geiman DE, Lane MD. CCAAT/enhancer binding protein gene promoter: binding of nuclear factors during differentiation of 3T3-L1 preadipocytes. P. Natl. Acad. Sci. USA 88: 2593-2597 (1991)   DOI
27 Kim YM, Kim DS, Chpi YS. Anticoagulant activities brown seaweed extracts in Korea. Food Sci. Biotechnol. 36: 1008-1013 (2004)   과학기술학회마을
28 Lamela M, Anca J, Villar R, Otero J, Calleja JM. Hypoglycemic activity of several seaweed extracts. J. Ethnopharmacol. 27: 35-43 (1989)   DOI   ScienceOn
29 Shepherd PR, Gnudi L, Tozzo E, Yang H, Leach F, Kahn BB. Adipose cell hyperplasia and enhanced glucose disposal in transgenic mice overexpressing GLUT4 selectively in adipose tissue. J. BioI. Chem. 268: 22243-22246 (1993)
30 Li N, Zhang Q, Song J. Toxicological evaluation of fucoidan extracted from Larninaria japonica in Wistar rats. Food. Chem. Toxicol. 43: 421-426 (2005)   DOI   ScienceOn
31 McKeon C, Pham T. Transactivation of the human insulin receptor gene by the CAAT/enhancer binding protein. Biochem. Bioph. Res. Co. 174: 721-728 (1991)   DOI   ScienceOn
32 Birkenmeier EH, Gwynn B, Howard S, Jerry J, Gordon JI, Landschulz WH, McKnight SL. Tissue-specific expression, developmental regulation, and genetic mapping of the gene encoding CCAAT/enhancer binding protein. Gene Dev. 3: 1146-1156 (1989)   DOI   ScienceOn
33 Zisman A, Peroni OD, Abel ED, Michael MD, Mauvais-Jarvis F, Lowell BB, Wojtaszewski JF, Hirshman MF, Virkamaki A, Goodyear LJ. Targeted disruption of the glucose transporter 4 selectively in muscle causes insulin resistance and glucose intolerance. Nat. Med. 6: 924-928 (2000)   DOI   ScienceOn
34 Wu Z, Rosen ED, Brun R, Hauser S, Adelmont G, Troy AE, McKeon C, Darlington GJ, Spiegelman BM. Cross-regulation of C/EBP$\alpha$ and PPAR$\gamma$ controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol. Cell 3: 151-158 (1999)   DOI   ScienceOn
35 Blondin C, Chaubet F, Nardella A, Sinquin C, Jozefonvicz J. Relationships between chemical characteristics and anticomplementary activity of fucans. Biomaterials 17: 597-603 (1996)   DOI   ScienceOn
36 Bryant NJ, Govers B, Hane DE. Regulated transport of the glucose transporter GLUT4. Nat. Rev. Mol. Cell. BioI. 3: 267-277 (2002)   DOI   ScienceOn
37 Student AK, Hsu RY, Lane MD. Induction of fatty acid synthetase synthesis in differentiating 3T3-L1 preadipocytes. J. BioI. Chem. 255: 4745-4750 (1980)
38 Garvey WT, Maianu L, Zhu JH, Brechtel-Hook G, Wallace P, Baron AD. Evidence for defects in the trafficking and translocation of GLUT4 glucose transporters in skeletal muscle as a cause of human insulin resistance. J. Clin. Invest. 101: 2377-2386 (1998)   DOI   ScienceOn
39 He W, Barak Y, Hevener A, Olson P, Liao D, Le J, Nelson M, Ong E, Olefsky JM, Evans RM. Adipose-specific peroxisome proliferatoractivated receptor gamma knockout causes insulin resistance in fat and liver but not in muscle, P. Natl. Acad. Sci. USA 100: 15712-15717 (2003)   DOI   ScienceOn
40 Beress A, Wassermann O, Tahhan S, Bruhn T, Beress L, Kraiselburd EN. A new procedure for the isolation of anti-HlV compounds (polysaccharide and polyphenols) from the marine algae Fucus vesiculosus. J. Nat. Prod. 56: 478-488 (1993)   DOI   ScienceOn
41 Willson TM, Cobb JE, Cowan DJ. The structure-activity relationship between peroxisome proliferators-activated receptor gama agonism and the antihyperglycemic activity of thiazolidinediones. J. Med. Chem. 39: 665-668 (1996)   DOI   ScienceOn
42 Lee JB, Hayashi K, Hashimoto M, Nakano T, Hayashi T. Novel antiviral fucoidan from sporophyll of Undaria pinnatifida (Mekabu). Chem. Pharm. Bull. 52: 1091-1094 (2004)   DOI   ScienceOn
43 Christy RJ, Tang VW, Ntambi JM, Geiman DE, Landschulz WH. Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes. Gene Dev. 3: 1323-1335 (1989)   DOI   ScienceOn