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A Dipstick-Type Enzyme-Linked Immunosorbent Assay for the Detection of the Insecticide Fenitrothion in Food Samples  

Cho, Young-Ae (Department of Food Science and Nutrition, Kyungpook National University)
Shim, Jee-Youn (Department of Food Science and Nutrition, Kyungpook National University)
Lee, Yong-Tae (Department of Biochemistry, Yeungnam University)
Lee, Hye-Sung (Department of Food Science and Nutrition, Kyungpook National University)
Publication Information
Food Science and Biotechnology / v.15, no.6, 2006 , pp. 990-992 More about this Journal
Abstract
In a previous study, we obtained polyclonal antibodies against the organophosphorus insecticide fenitrothion and developed an enzyme-linked immunosorbent assay (ELISA) for this pesticide. Using these antibodies and an enzyme tracer, a direct competitive ELISA method specific for fenitrothion using a dipstick format was developed. Dipstick ELISA using antibodies to fenitrothion immobilized on an Immunodyne membrane allowed the quick visual detection of fenitrothion at concentrations above $10\;{\mu}g/L$. The $IC_{50}$ value of dipstick ELISA using reflectance detection was $27\;{\mu}g/L$ with a detection limit of $2\;{\mu}g/L$. The recovery of fenitrothion from spiked lettuce and rice samples using the dipstick ELISA ranged from 87-107%.
Keywords
fenitrothion; insecticide; enzyme-linked immunosorbent assay; dipstick;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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