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Characteristics of a Bifidobacterium longum LL04 ${\beta}$-Galactosidase (recombinant) Produced in Escherichia coli  

Lim, Seong-Il (Division of Fermented Food Research, Korea Food Research Institute)
Kim, Geun-Bae (Department of Animal Science and Technology, Chung-Ang University)
Yi, Sung-Hun (Department of Food Science and Agricultural Chemistry, McGill University)
Lee, Byong-Hoon (Department of Food Science and Agricultural Chemistry, McGill University)
Publication Information
Food Science and Biotechnology / v.15, no.6, 2006 , pp. 908-913 More about this Journal
Abstract
Recombinant ${\beta}$-galactosidase from Bifidobacterium longum LL04 was expressed in Escherichia coli and partially purified by ammonium sulphate precipitation and anion-exchange chromatography (Mono-Q). The optimum temperature and pH of the partially purified enzyme were $50^{\circ}C$ and pH 7.0-8.0, respectively, when o-nitrophenyl-${\beta}$-D-galactopyranoside was used as a substrate. The enzyme was stable over the pH range of 5.0-9.0, and was active at $40^{\circ}C$ for more than 60 min at pH 7.0. The enzyme was significantly activated by $Na^+$ and $K^+$. Maximal activity was observed at the concentration of 10 mM for both $Na^+$ and $K^+$. The enzyme activity was strongly inhibited by most bivalent metal ions. The Km and Vmax on ONPG at 37 and $50^{\circ}C$ were 0.72, 167.9, and 0.507 mM, 310.9 U/mL, respectively.
Keywords
Bifidobacterium longum; recombinant ${\beta}$-galactosidase; characteristics;
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1 Kim JW, Lee SJ, Park KH. Antimicrobial effect of Bifidobacterium breve and Bijidobacterium infantis against Salmonella typhimurium KCTC 1925 and E. coli O157:H7 ATCC 43895. Food Sci. Biotechnol. 11: 89-92 (2002)
2 Toida T, Sekine K, Tatsuki T, Saito M, Kawashima T, Hashimoto Y, Sakurai Y. Biochemical characterization and antitumor activity of a new cell wall preparation, whole peptidoglycan (WPG) from Bijidobacterium infantis. J. Cancer Res. Clin. 116: 342-348 (1990)
3 Tahri K, Grill JP, Schneider F. Involvement oftrihydroxyconjugated bile salts in cholesterol assimilation by bifidobacteria. Curr. Microbiol. 34: 79-84 (1997)   DOI
4 Deguchi Y, Morishita T, Mutai M. Comparative studies on systhesis of water-soluble vitamins among human species of bifidobacteria. Agr. Biol. Chem. Tokyo 49: 13-19 (1985)   DOI
5 Rossi M, Altomare L, Gohzalez A, Roodriguez V, Brigidi P, Matteuzzi D. Nucleotide sequence, expression, and transcriptional analysis of the Bifidobacterium longum MB 219 lacZ gene. Arch. Michrobiol. 174: 74-80 (2000)   DOI
6 Huang DQ, Prevost H, Divies C. Principal characteristics of $\beta$-galactosidase from Leuconostoc spp. Int. Dairy J. 5: 29-43 (1995)   DOI   ScienceOn
7 Ulrich JT, McFeter GA, Temple KL. Induction and characterization of $\beta$-galactosidase in an extreme thermophile. J. Bacteriol. 110: 691-698 (1972)
8 Mahoney RR, Nickerson TA, Whitaker JR. Selection of strain, growth conditions and extraction procedures for optimum production of lactase from Kluyveromyces fragilis. J. Dairy Sci. 58: 1620-1629 (1975)   DOI   ScienceOn
9 Abdelrahim KA, Lee BH. Production and characterization of $\beta$-galactosidase from psychrotrophic Bacillus subtilis KL88. Biotechnol. Appl. Bioc. 13: 246-256 (1991)
10 Garman J, Coolbear T, Smart J. The effect of cations on the hydrolysis of lactose and the transferase reactions catalysed by $\beta$-galactosidase from six strains of lactic acid bacteria. Appl. Microbiol. Biot. 46: 22-27 (1996)   DOI
11 Trimbur DE, Gutshall KR, Prema P, Brenchley JE. Characterization of a psychrotrophic arthrobacter gene and its cold-active $\beta$-galactosidase. Appl. Environ. Microbiol. 60: 4544-4552 (1994)
12 Greenberg NA, Mahoney RR. Production and characterization of $\beta$-galactosidase from Streptococcus thermophilus. J. Food Sci. 47: 1824-1828 (1982)   DOI
13 Huber RE, Kurz G, Wallenfels K. A quantitation of the factor which affect the hydrolase and transgalactosylase activities of $\beta$-galactosidase (E. coli) on lactose. Biochemistry-US 15: 1994-2001 (1976)   DOI   ScienceOn
14 Hung MN, Lee BH. Cloning and expression of $\beta$-galactosidase genes from Bifidobacterium irfantis into Escherichia coli. Biotechnol. Lett. 20: 659-662 (1998)   DOI   ScienceOn
15 Greenberg NA, Mahoney RR. Immobilization of lactose ($\beta$galactosidase) for use in dairy processing: A Review. Process Biochem. 16: 2-8 (1981)
16 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 72: 248-254 (1970)   DOI   ScienceOn
17 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 (1970)   DOI   ScienceOn
18 Bhowmik T, Johnson MC, Ray B. Factors influencing synthesis and activity of $\beta$-galactosidase in Lactobacillus acidophilus. J. Ind. Microbiol. 2: 1-7 (1987)   DOI
19 Park YK, DeSanti MSS, Pastore OM. Production and characterization of $\beta$-galactosidase from Aspergillus oryzae. J. Food Sci. 44: 100-103 (1979)   DOI
20 Nunoura N, Ohdan K, Tanaka K, Tamaki H, Yano T, Inui M, Yukawa H, Yamamoto K, Kumagai H. Cloning and nucleotide sequence of the $\beta$-D-galactosidase gene from Bifidobacterium breve clb, and expression of $\beta$-D-galactosidase activity in Escherichia coli. Biosci. Biotech. Bioch. 60: 2011-2018 (1996)   DOI   ScienceOn
21 Dumortier V, Brassart C, Bouquelet S. Purification and properties of a $\beta$-D-galactosidase from Bifidobacterium bifidum exhibiting a transgalactosylation reaction. Biotechnol. Appl. Bioc. 19: 341-354 (1994)
22 Schell MA, Karrnirantzou M, Snel B, Vilanova D, Berger B, Pessi G, Zwahlen MC, Desiere F, Bork P, Delley M, Pridmore RD, Arigoni F. The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. P. Natl. Acad. Sci. USA 99: 14422-14427 (2002)
23 Cavaille D, Combes D. Characterization of $\beta$-galactosidase from Kluyveromyces lactis. Biotechnol. Appl. Biochem. 22: 55-64 (1995)
24 Tahri K, Grill JP, Schneider F. Bifidobacteria strain behavior toward cholesterol: coprecipitation with bile salts and assimilation. Curr. Microbiol. 33: 187-193 (1996)   DOI
25 Chakraborti S, Sani RK, Banerjee UC, Sobti RC. Purification and characterization of a novel $\beta$-galactosidase from Bacillus sp MTCC 3088. J. Ind. Microbiol. Biot. 24: 58-63 (2000)   DOI
26 Shin MS, Yu KW, Shin KS, Lee H. Enhancement of immunological activity in mice with oral administration of cell wall components of Bijidobacterium infidum. Food Sci. Biotechnol. 13: 85-89 (2004)
27 Bhowmik T, Marth EH. $\beta$-Galactosidase of Pedococcus species: induction, purification and partial characterization. Appl. Microbiol. Biot. 33: 317-323 (1990)   DOI
28 Meghrous J, Euloge P, Junelles AM, Ballongue J, Petitdemange H. Screening of Bijidobacterium strains from bacteriocin production. Biotechnol. Lett. 12: 575-580 (1990)   DOI
29 Bouhnik Y, Flourie B, Andrieux C, Bisetti N, Briet F, Rambaud rc. Effects of Bijidobacterium sp. fermented milk ingested with or without inulin on colonic bifidobacteria and enzymatic activities in healthy humans. Eur. J. Clin. Nutr. 50: 269-273 (1996)
30 Hung MN, Lee BH. Purification and characterization of a recombinant $\beta$-galactosidase with transgalactosylation activity from Bifidobacterium infantis HL96. Appl. Microbiol. Biochem. 58: 439-445 (2002)   DOI
31 Smart J, Richardson B. Molecular properties and sensitivity to cations of $\beta$-galactosidase from Streptococcus thermophilus with four enzyme substrates. Appl. Microbiol. Biot. 26: 177-185 (1987)   DOI
32 Nakao M, Harada M, Kodama Y, Nakayama T, Shibano Y, Amachi T. Purification and characterization of a thermostable $\beta$-galactosidase with high transgalactosylation activity from Saccharopolyspora rectivirgula. Appl. Microbiol. Biot. 40: 657-663 (1994)   DOI
33 Fujiwara S, Hashiba H, Hirota T, Forstner JF. Proteinaceous factor(s) in culture supernatant fluids of bifidobacteria which prevents the binding of enterotoxigenic Escherichia coli to gangliotetraosylceramide. Appl. Environ. Microbiol. 63: 506-512 (1997)
34 Singh J, Rivenson A, Tomita M, Shimamura S, Ishibashi N, Reddy BS. Bijidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Carcinogenesis 18: 833-841 (1997)   DOI   ScienceOn
35 Itoh T, Ohhashi M, Toba T, Adachi S. Purification and properties of $\beta$-galactosidase from Lactobacillus bulgaricus. Milchwissenschaft 35: 593-597 (1980)
36 Teraguchi S, Ono J, Kiyosawa I, Fukuwatari Y, Araki J, Okonogi S. Vitamin production by bifidobacteria originated from human intestine. J. Jpn. Soc. Nutr. Food Sci. 32: 157-169 (1986)
37 Ohtsuka K, Tanoh A, Ozawa O, Kanematsu T, Uchida T, Shinke R. Purification and properties of a $\beta$-galactosidase with high galactosyl transfer activity from Cryptococcus laurentii. J. Ferment. Bioeng. 70: 301-307 (1990)   DOI   ScienceOn