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Characterization of Lactobacillus cellobiosus D37 Isolated from Soybean Paste as a Probiotic with Anti-Cancer and Antimicrobial Properties  

Lim, Sung-Mee (Department of Food Science and Technology, Tongmyong University)
Lee, Goon-Ja (Department of Food Science and Technology, Tongmyong University)
Park, Sun-Mee (Epidemiology Division, Busan Metropolitan City Institute of Health and Environment)
Ahn, Dong-Hyun (Department of Food Science and Technology, Pukyong National University)
Im, Dong-Soon (College of Pharmacy and Research Institute for Drug Development, Pusan National University)
Publication Information
Food Science and Biotechnology / v.15, no.5, 2006 , pp. 792-798 More about this Journal
Abstract
The probiotic characteristics of a total of 137 lactic acid bacterial strains isolated from soybean paste were investigated. Among those tested, the D37 strain was selected as a probiotic bacteria due to its acid and bile tolerance, and its strong anti-cancer and antibacterial activities. The D37 strain showed highly stable viability at acidic pH for 2 hr, and was very stable in 10% bovine bile. The viability of human colon cancer HT-29 cells was inhibited more than 60% at a $200\;{\mu}/mL$ concentration of D37 cell-free culture supernatant, and the degree of inhibition was concentration-dependent. The D37 strain showed a wide range of antibacterial activities against food-borne pathogenic bacteria such as Escherichia coli O157, Listeria spp., Vibrio spp., Salmonella spp., and Staphylococcus aureus. According to phenotypic characteristics and the utilization of various sugars, the D37 strain was identified as Lactobacillus cellobiosus.
Keywords
soybean paste; acid and bile tolerance; anti-cancer activity; antimicrobial activity; Lactobacillus cellobiosus;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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1 Gill HS, Guarner F. Probiotics and human health: a clinical perspective. Postgrad. Med. J. 80: 516-526 (2006)
2 Simonetta AC, Moragues LG, Frison LN. Antibacterial activity of enterococci strains against Vibrio cholerae. Lett. Appl. Microbiol. 24: 139-143 (1997)   DOI   ScienceOn
3 Sgouras D, Maragkoudakis P, Petraki K, Martinez-Gonzalez B, Eriotou E, Michopoulos S, Kalantzopoulos G, Tsakalidou E, Mentis A. In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus casei strain Shirota. Appl. Environ. Microbiol. 70: 518-526 (2004)   DOI
4 Ouwehand AC, Kirjavainen PV, Shortt C, Salminen S. Probiotics: mechanisms and established effects. Int. Dairy J. 9: 43-52 (1999)   DOI   ScienceOn
5 Park KY, Moon SH, Cheigh HS, Baik HS. Antimutagenic effects of doenjang (Korean soy paste). J. Food Sci. Nutr. 1: 151-158 (1996)
6 Parente E, Brienza C, Moles M, Ricciardi A. A comparison of methods for the measurement of bacteriocin activity. J. Microbiol. Meth. 22: 95-108 (1995)   DOI   ScienceOn
7 Lee JW, Shin JG, Kim EH, Kang HE, Yim IB, Kim JY, Joo HG, Woo HJ. Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum. J. Vet. Sci. 5: 41-48 (2004)
8 Sanders ME, Klaenhammer TR. The scientific basis of Lactobacillus acidophilus NCFM functionality as a probiotic. J. Dairy Sci. 84: 319-331 (2001)   DOI   ScienceOn
9 Tagg JR, Dajani AS, Wannamaker LW. Bacteriocins of Grampositive bacteria. Bacteriol. Rev. 40: 722-756 (1976)
10 Sameshima T, Magome C, Takeshita K, Arihara K, Itoh M, Kondo Y. Effect of intestinal Lactobacillus starter cultures on the behaviour of Staphylococcus aureus in fermented sausage. Int. J. Food Microbiol. 41: 1-7 (1998)   DOI   ScienceOn
11 Dunne C, O'Mahony L, Murphy L, Thornton G, Morrissey D, O'Halloran S, Feeney M, Flynn S, Fitzgerald G, Daly C, Kiely B, O'Sullivan GC, Shanahan F, Collins JK. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386-392 (2001)   DOI
12 Tsai CC, Hsih HY, Chiu HR, Lai YY, Liu JH, Yu B, Tsen HY. Antagonistic activity against Salmonella infection in vitro and in vivo for two Lactobacillus strains from swine and poultry. Int. J. Food Microbiol. 102: 185-194 (2005)   DOI   ScienceOn
13 Ogawa M, Shimizu K, Nomot K, Tanaka R, Hamabata T, Yamasaki S, Takeda T, Takeda Y. Inhibition of in vitro growth of shiga toxinproducing Escherichia coli O157:H7 by probiotic Lactobacillus strains due to production of lactic acid. Int. J. Food Microbiol. 68: 135-140 (2001)   DOI   ScienceOn
14 Lash BW, Mysliwiec TH, Gourama H. Detection and partial characterization of a broad-range bacteriocin produced by Lactobacillus plantarum (ATCC 8014). Food Microbiol. 22: 199-204 (2005)   DOI   ScienceOn
15 Vinderola CG, Reinheimer JA. Lactic acid starter and probiotic bacteria: a comparative 'in vitro' study of probiotic characteristics and biological barrier resistance. Food Res. Int. 36: 895-904 (2003)   DOI   ScienceOn
16 Zhao T, Doyle MP, Harmon BG, Brown CA, Muellere PO, Parks AH. Reduction of carriage of enterohemorrhagic Escherichia coli O157:H7 in cattle by inoculation with probiotic bacteria. J. Clin. Microbiol. 36: 641-647 (1998)
17 Commane D, Hughes R, Shortt C, Rowland I. The potential mechanisms involved in the anti-carcinogenic action of probiotics. Mutat. Res. 591: 276-289 (2005)   DOI
18 Goldin B, Gualtieri L, Moore R. The effect of Lactobacillus GG on the initiation and promotion of DMH induced intestinal tumors in the rat. Nutr. Cancer. 25: 197-204 (1996)   DOI
19 Parker SL, Tong T, Bolden S, Wingo PA. Cancer statistics. CA-Cancer J. Clin. 47: 5-27 (1997)   DOI   ScienceOn
20 Rafter J. Probiotics and colon cancer. Best Pract. Res. Cl. Ga. 17: 849-859 (2003)
21 Kim YS, Rhee CH, Park HD. Isolation and characterization of a bacterium from Korean soy paste doenjang producing inhibition of angiotension converting enzyme. Korean. J. Food Sci. Technol. 33: 84-88 (2001)
22 Fuller R. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365-371 (1988)
23 Yoo SK, Cho WH, Kang SM, Lee SH. Isolation and identification of microorganisms in Korean traditional soybean paste and soybean sauce. Korean J. Appl. Microbiol. Biotechnol. 27: 113-117 (1999)
24 Takagi A, Matsuzaki T, Sato M, Nomoto K, Morotomi M, Yokokura T. Enhancement of natural killer cytotoxicity delayed murine carcinogenesis by a probiotic microorganism. Carcinogenesis 22: 599-605 (2001)   DOI   ScienceOn
25 Okereke A, Montville T. Bacteriocin inhibition of Clostridium botulinum spores by lactic acid bacteria. J. Food Protect. 54: 349-353 (1991)   DOI
26 Hirayama K, Rafter J. The role of probiotic bacteria in cancer prevention. Microbes Infect. 2: 681-686 (2000)   DOI   ScienceOn
27 Kwon Sh, Shon MY. Antioxidant and anticarcinogenic effects of traditional doenjang during maturation periods. Korean. J. Food Pres. 11: 461-467 (2004)   과학기술학회마을
28 Lim SM, Park MY, Chang DS. Characterization of bacteriocin produced by Enterococcus faecium MJ-14 isolated from meju. Food Sci. Biotechnol. 14: 49-57 (2005)
29 Andersson R. Inhibition of Staphylococcus aureus and spheroplasts of Gram-negative bacteria by an antagonistic compound produced by a strain of Lactobacillus plantarum. Int. J. Food Microbiol. 3: 149-160 (1986)   DOI   ScienceOn
30 Pool-Zobel BL, Neudecker C, Domizlaff I, Ji S, Schillinger U, Rumney C, Moretti M, Vilarini I, Scassellati-Sforzolini R, Rowland I. Lactobacillus- and Bifidobacterium-mediated antigenotoxicity in the colon of rats. Nutr. Cancer. 26: 365-380 (1996)   DOI   ScienceOn
31 Pedersen C, Jonsson H, Lindberg JE, Roos S. Microbiological characterization of wet wheat distillers' grain, with focus on isolation of Lactobacillus with potential as probiotics. Appl. Environ. Microbiol. 70: 1522-1527 (2004)   DOI
32 Fuller R. Probiotics: The scientific basis. Chapman & Hall, London, UK. pp. 209-221 (1992)
33 Kim SJ. Potential probiotic properties of lactic acid bacteria isolated from kimchi. Food Sci. Biotechnol. 14: 547-550 (2005)
34 Erickson K, Hubbard N. Probiotic immunomodulation in health and disease. J. Nutr. 130: 403-409 (2000)
35 Heller KJ. Probiotic bacteria in fermented foods: product characteristics and starter organisms. Am. J. Clin. Nutr. 73: 374-379 (2001)   DOI
36 Mundt JO. Enterococci. Vol. 2, pp. 1063-1065. In: Bergey's Manual of Systematic Bacteriology. Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds). Williams & Wilkins, Baltimore, MD, USA (1986)
37 Chang YH, Kim JK, Kim HJ, Kim WY, Kim YB, Park YH. Probiotic effects of Lactobacillus reuteri BSA-131 on piglets. Korean J. Appl. Microbiol. Biotechnol. 28: 8-13 (2000)   과학기술학회마을
38 El-Nezami H, Mykkanen H, Kankaanpaa P, Salminen S, Ahokas J. Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B, from the chicken duodenum. J. Food Protect. 63: 549-552 (2000)   DOI
39 Waard R, Garssen J, Bokken GCAM, Vos JG. Antagonistic activity of Lactobacillus casei strain Shirota against gastrointestinal Listeria monocytogenes infection in rats. Int. J. Food Microbiol. 73: 93-100 (2002)   DOI   ScienceOn
40 Mombelli B, Gismondo MR. The use of probiotics in medical practice. Int. J. Antimicrob. Ag. 16: 531-536 (2000)   DOI   ScienceOn