Browse > Article

Characterization and Application of a Novel Thermostable Glucoamylase Cloned from a Hyperthermophilic Archaeon Sulfolobus tokodaii  

Njoroge, Rose Nyawira (Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University)
Li, Dan (National R&D Center for Soybean Processing, and College of Biological Science and Technology, Changchun University)
Park, Jong-Tae (Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University)
Cha, Hyun-Ju (Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University)
Kim, Mi-Sun (Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University)
Kim, Jung-Wan (Department of Biology, University of Incheon)
Park, Kwan-Hwa (Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University)
Publication Information
Food Science and Biotechnology / v.14, no.6, 2005 , pp. 860-865 More about this Journal
Abstract
A gene for a putative glucoamylase, stg, of a hyperthermophilic archae on Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The recombinant glucoamylase (STGA) had an optimal temperature of $80^{\circ}C$ and was extremely thermostable with a D-value of 17 hr. The pH optimum of the enzyme was 4.5. Being different from fungal glucoamylases, STGA hydrolyzed maltotriose (G3) most efficiently. Gel permeation chromatography and sedimentation equilibrium analytical ultracentrifugation analysis showed that the enzyme existed as a dimer. STGA was stable enough to hydrolyze liquefied com starch to glucose in 4 hr at $90^{\circ}C$ with a yield of95%. Comparison of the $k_{cat}$ values for the hydrolysis and the reverse reaction at $75^{\circ}C$ and $90^{\circ}C$ indicated that glucose production by STGA was more efficient at $90^{\circ}C$ than $75^{\circ}C$. Therefore, STGA showed great potential for application to the industrial glucose production process due to its high thermostability.
Keywords
Sulfolobus tokodaii glucoamylase (STGA); reversion; thermostability;
Citations & Related Records

Times Cited By Web Of Science : 4  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Properties of the recombinant <TEX>${\alpha}$</TEX>-glucosidase from Sulfolobus solfataricus in relation to starch processing /
[ Martino, A.;Schiraldi, C.;Fusco, S.;Di Lemia, I.;Costabile, T.;Pellicano, T.;Marotta, M.;Generoso, M.;van der Oost, J.;Sensen, C.W.;Charlebois, R.L.;Moracci, M.;Rossi, M.;De Rosa, M. ] / J. Mol. Catal. B-Enz
2 Amino acid, peptide, and protein volume in solution /
[ Zamyatnin, A. ] / Annu. Rev. Biophys. Bioeng.
3 Glucoamylase: structure/function relationships, and protein engineering /
[ Sauer, J.;Sigurskjold, B.W.;Christensen, U.;Frandsen, T.P.;Mirgorodskaya, E.;Harrison, M.;Roepstorff, P.;Svensson, B. ] / Biochim. Biophys. Acta
4 Crystal structure and evolution of a prokaryotic glucoamylase /
[ Aleshm, A.E.;Feng, P.H.;Honzatko, R.B.;Reilly, P.J. ] / J. Mol. Biol.
5 Properties of a novel thermostable glucoamylase from the hyperthermophilic archaeon, Sulfolobus solfataricus, in relation to starch processing /
[ Kim, M.S.;Park, J.T.;Kim, Y.W.;Lee, H.S.;Nyawira, R.;Shin, H.S.;Park, C.S.;Yoo, S.H.;Kim, Y.R.;Moon, T.W.;Park, K.H. ] / Appl. Environ. Microbiol.
6 Characterization of an archaeal cyclodextrin glucanotransferase With a novel C-terminal domain /
[ Rashid, N.;Comista, J.;Ezaki, S.;Fukui, T.;Atomi, H.;Imanaka, T. ] / J. Bacteriol.
7 Purification and characterization of an extremely thermostable cyclomaltodextrin glucano？transferase from a newly isolated hyperthermophilic archaeon, a Thermococcus sp /
[ Tachibana, Y.;Kuramura, A.;Shirasaka, N.;Suzuki, Y.;Yamamoto, T.;Fujiwara, S.;Takagi, M.;Imanaka, T. ] / Appl. Environ. Microbiol.
8 Cloning and expression of the a-amylase gene from the hypertherthermophilic archaeon Pyrococcus sp. KOD1, and characterization of the enzyme /
[ Tachibana, Y.;Leclere, M.M.;Fujiwara, S.;Takagi, M.;Imanaka, T. ] / J. Ferment. Bioeng.
9 Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7 /
[ Kawarabayasi, Y.;Hino, Y.;Horikawa, H.;Kim, I.N.;Takahashi, M.;Sekine, M.;Baba. S.;Ankai, A.;Kosugi, H.;Hosoyama, A.;Fukui, S.;Nagai, Y.;Nishijima, K.;Otsuka, R.;Nakazawa, H.;Takamiya, M.;Kato, Y.;Yoshlzawa, T.;Tanaka, T.;Kudoh, Y.;Yamazaki, J.;Kushida, N.;Oguchi, A.;Aoki, K.;Masuda, S.;Yanagii, M.;Nishimura, M.;Yamagishi, A.;Oshima, T.;Kikuchi, H. ] / DNA Res.
10 Modulation of Bacillus licheniformis amylases by Site-directed and deleted mutagenesis /
[ Cheong, T.K. ] /
11 Molecular cloning: a laboratory manual /
[ Sambrook, J.;Fritsch, E.F.;Maniatis, T. ] /
12 Cleavage of structural proteins during the assembly of the head of bacteriophage <TEX>$T_4$</TEX> /
[ Laemmli, U.K. ] / Nature
13 Miniaturization of three carbohydrate analyses usmg a mlcrosample plate reader /
[ Fox, J.D.;Robyt, J.F. ] / Anal. Biochem.
14 Glucoarnylase from Thermom:aerobacterium thennosacchamlyticum: Sequence studies and analysIs of the macromolecular architecture of the enzyme /
[ Ducki, A.;Grundmann, O.;Konermann, L.;Mayer, F.;Hoppert, M. ] / J. Gen. Appl. Microbiol.
15 Production, punfication, and characterization of Thermoanaerobacterium thermosaccharolyticum glucoamylase /
[ Feng, P.H.;Berensmeier, S.;Buchholz, K.;Reilly, P.J. ] / Starch/Strke
16 Novel thermoactive glucoamylases from the thermo~Cldophilic Archaea Thennoplasma acidophilum, Pzcrophzlus tomdus and Picrophilus oshimae /
[ Serour, E.;Antranikian, G. ] / Antonie Van Leeuwenhoek
17 Novel glucoamylase-type enzymes from Thermoactinomyces vulgaris and Methanococcus jannaschii whose genes are found in the flanking region of the a-am lyase genes /
[ Uotsu-Tomita, R.;Tonozuka, T.;Sakai, H.;Sakano, Y. ] / Appl. Microbiol. Biotechnol.
18 Regulation and genetic enhancement of glucoamylase and pullulanase production in Clostridium then:lOhydrosulfuricum /
[ Hyun, H.H.;Zeikus, J.G. ] / J. Bacteriol.
19 Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme /
[ Ohnlsh, H.;Kitamura, H.;Mmowa, T.;Sakai, H.;Ohta, T. ] / Eur. J. Biochem.
20 Starch Conversion;Industrial Enzymology /
[ Bentley, I.S.;Williams, E.C.;Godfrey, T.(ed.);West, S.(ed) ] /
21 Starch-hydrolyzing enzymes from thermophilic archaea and bacteria /
[ Bertoldo, C.; Antranikian, G. ] / Curr. Opin. Chem. Biol.
22 Biotechnological uses of archaeal extremozymes /
[ Eichler, J. ] / Biotechnol. Adv.
23 Extremozymes /
[ Hough, D.W.;Danson, M.J. ] / Curro Opin. Chem. Biol.
24 Very stable enzymes from extremely thermophilic archaebacteria and eubacteria /
[ Bragger, J.M.;Daniel, R.M.;Coolbear, T.;Morgan, H.W. ] / Appl. Microbiol. Biotechnol.
25 Purification and properties of extracellular amylase from the hyperther mophilic archaeon Thermococcus pmfundus DT5432 /
[ Chung, Y.C.;Kobayashi, T.;Kanai, H.;Akiba, T.;Kudo, T. ] / Appl. Environ. Microbiol.
26 Extremely thermophilic aCidophilic bactena convergent with Sulfolobus acidocaldarius /
[ de Rosa, M.;Gambacorta, A.;Bu'lock, J.D. ] / J. Gen. Microbiol.   ScienceOn
27 Improving operating performance of glucoamylase by mutagenesis /
[ Ford, C. ] / Curr. Opin. Biotechnol.
28 Protein engineering of glucoamylase to improve mdustnal performance-a review /
[ Reilly, P.J. ] / Starch
29 Fungal glucoamylase /
[ Manjunath, P.;Shenoy, B.C.;Raghavendra Rao, M.R. ] / J. Appl. Biochem.