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http://dx.doi.org/10.3839/jabc.2022.026

HPLC-UVD method validation for quantitative analysis of camelliaside A in hot-water extract of soybean (Glycine max L.) leaves  

Kim, Jeong Ho (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University)
Lee, Seung Hwan (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University)
Moon, Si Won (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University)
Park, Ki Hun (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.3, 2022 , pp. 195-202 More about this Journal
Abstract
Soybean (Glycine max L.) leaves have been researched as functional food stuff actively, but there is no validation method to control quality of soybean leaves (SL). In this study, we annotated seven kaempferol derivatives to confirm camelliaside A as index metabolite in SL using UHPLC-ESI-TOF-MS. HPLC-UVD validation method of camelliaside A in hot-water extract of SL was established according to validation guideline of functional foods from the Ministry of Food and Safety of Korea. The HPLC-UVD method was validated with reliable parameters for examining specificity, accuracy, precision, limit of detection and quantification and linearity. The established method gave the suitable ranges to qunatitate camelliaside A from the hot-water extract of soybean leaves.
Keywords
Camelliaside A; HPLC-UVD; Soybean leaves; Validation;
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