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http://dx.doi.org/10.3839/jabc.2022.020

Phytochemical constituents of Lactuca serriola leaves and their content analysis by HPLC-UV  

Kim, Juree (Department of Plant Science and Technology, Chung-Ang University)
Lee, Hak-Dong (Department of Plant Science and Technology, Chung-Ang University)
Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University)
Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.3, 2022 , pp. 153-158 More about this Journal
Abstract
This study aimed to identify the phytochemical constituents of Lactuca serriola leaves and perform quantitative analysis of the methanol (MeOH) extract of L. serriola, L. indica, L. raddeana, L. sativa, and L. triangulata. Six compounds were isolated from the MeOH extracts of L. serriola using open column chromatography and identified as protocatechuic acid (1), caffeic acid (2), cynaroside (3), apigenin 7-glucuronide (4), luteolin (5), and apigenin (6) using 1H-, 13C-nuclear magnetic resonance, and mass spectrometry. Quantitative analysis of the six compounds was performed on the MeOH extract of Lactuca species using high-performance liquid chromatography (HPLC) and an ultraviolet (UV). A reverse-phased column was used, and the UV absorbance was set to 280 nm. The contents of compounds 2 and 3 were the highest (1.58 and 2.64 mg/g ext., respectively) in L. serriola extracts. However, compounds 4 and 6 were higher (1.46 and 0.40 mg/g ext., respectively) in L. triangulata. These results provide quantitative data for the application of Lactuca species in the pharmaceutical and functional food industries.
Keywords
High-performance liquid chromatography-ultraviolet; Lactuca serriola; Quantitative analysis;
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