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http://dx.doi.org/10.3839/jabc.2020.055

Anti-inflammatory effect of a mixture of Astragalus membranaceus and Lithospermum erythrorhizon extracts by inhibition of MAPK and NF-κB signaling pathways in RAW264.7 cells  

Choi, Doo Jin (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kim, Geum Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Choi, Bo-Ram (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Han, Kyung Sook (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Dong-Sung (College of Pharmacy, Chosun University)
Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Applied Biological Chemistry / v.63, no.4, 2020 , pp. 421-428 More about this Journal
Abstract
This study investigated a mixture of Astragalus membranaceus (AM) and Lithospermum erythrorhizon (LE) extracts (ALM16), exerts anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells, and its underlying mechanism. ALM16 was prepared by mixing AM and LE extracts in a ratio of 7:3 (w/w). Cytotoxicity of ALM16 in RAW264.7 cells was not shown up to 200 ㎍/mL of ALM16. The results of this study showed that ALM16 does-dependently inhibits the production of nitric oxide, prostaglandin E2 and pro-inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor-α) in LPS-induced RAW264.7 cells. ALM16 not only markedly reduced the protein expression levels of inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW264.7 cells, but also inhibited the nuclear translocation and DNA-binding activity of nuclear factor-kappa B (NF-κB). In addition, ALM16 specifically inhibited the phosphorylation of c-Jun N-terminal kinase and extracellular signal-regulated kinases in LPS-stimulated RAW264.7 cells. In conclusion, these results suggest that ALM16 may exert anti-inflammatory effect by modulating mitogen-activated protein kinase and NF-κB signaling pathways.
Keywords
ALM16; Anti-inflammatory; Mitogen-activated protein kinase; Nuclear factor-kappa B; RAW264.7 macrophages;
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