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http://dx.doi.org/10.3839/jabc.2020.053

Analysis and stability test of the extracts from Ulmus parvifolia leaves  

Kim, Doo-Young (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Song, Soobin (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Iljoo (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jeong, Se-Kyoo (Research Division, Incospharm Corporation)
Kim, Sungwoo (Research Division, Incospharm Corporation)
Kim, Jung-Hee (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Jang, Hyun-Jae (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Oh, Sei-Ryang (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Ryu, Hyung Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Applied Biological Chemistry / v.63, no.4, 2020 , pp. 407-412 More about this Journal
Abstract
A simple and reliable HPLC method was developed to determine pharmacologically standard marker compounds of Ulmus parvifolia leaves. Standard markers were characterized with neochlorogenic acid (trans-5-O-caffeoylquinic acid, 5-CQA) and chlorogenic acid (trans-3-O-caffeoylquinic acid, 3-CQA) using NMR and UPLC-QTof-MS analysis. A method for qualitative/quantitative analysis of the leaves extracts were evaluated including two compounds by using HPLC. The stability test of 30% ethanolic extracts of the leaves sample and standard markers have been evaluated for six months. However, no significant changes in the content of the marker compounds of each extract was observed during the time of investigation.
Keywords
Caffeoylquinic acid; Stability; Ulmus parvifolia leaves; Ultra high performance liquid chromatography;
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