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http://dx.doi.org/10.3839/jabc.2020.051

Metabolic profiling and method validation of marker compounds from Saposhnikoviae Radix and Peucedani Japonici Radix  

Choi, Bo-Ram (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Yoon, Dahye (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Han, Kyung-Sook (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Choi, Doo Jin (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Hyun, Do Yoon (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Applied Biological Chemistry / v.63, no.4, 2020 , pp. 393-399 More about this Journal
Abstract
Saposhnikoviae Radix (SR) and Peucedani Japonici Radix (PR) have been used as the main traditional herbal medicines in Korea, China and Japan. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied to evaluate the quality of SR and PR using the marker compounds. In the S-plot of SR and PR, 5-O-methylvisammioside and peucedanol were selected as a marker compound for SR and PR, respectively. Also, an UPLC method was established and well validated for marker compounds of SR and PR. These results suggested that the established analysis method could be used one of the good methods for the classification and quality assessment of SR and PR.
Keywords
Metabolic profiling; Method validation 5-O-methylvisammioside; Peucedani Japonici Radix; Peucedanol; Saposhnikoviae Radix;
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